148 



ORDER I. PSEUDOMONADALES 



Beef-peptone agar colonies: Round, 

 smooth, shining, white to pale yellow. 



Milk: Peptonized. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from carbohydrates. 



Optimum temperature between 23° and 

 25° C. 



Aerobic. 



Habitat: Causes a root rot of red clover 

 {Trifolium praiense) , lentils (Lens escu- 

 lenta) and lucerne. 



137. Pseudomonas cattleyae (Pava- 

 rino, 1911) SS,vulescu, 1947. {Bacterium 

 cattleyae Pavarino, Atti R. Acad. Naz. 

 Lincei Rend. CI. Sci. Fis., Mat. e Nat., 20, 

 1911, 233; Savulescu, Anal. Acad. Romane, 

 III, 22, 1947, 11.) 



catt'ley.ae. M.L.fem.noun Cattleya a 

 generic name; M.L. gen. noun cattleyae of 

 Cattleya. 



Description from Ark and Thomas (Phy- 

 topath., 36, 1946, 697). 



Rods, 0.4 to 0.6 by 2.4 microns, occurring 

 singly or in pairs. Motile by means of 1 or 

 2 bipolar flagella. Gram-negative. 



Gelatin: No liquefaction. 



Beef -extract peptone agar colonies: 

 Large, entire, smooth, with criss-cross 

 markings. Grayish white and butyrous. 



Broth: Turbid in 24 hours with very deli- 

 cate pellicle. 



Fermi's, Cohn's, and Uschinsky's solu- 

 tions: Good growth. 



Hydrogen sulfide not produced. 



Indole not produced. 



Nitrites produced from nitrates. 



Litmus milk: Unchanged after 2 weeks. 



Acid but no gas from glucose, galactose, 

 fructose, arabinose, xylose, lactose, sucrose, 

 dulcitol, glycerol and mannitol. No acid or 

 gas from raffinose. 



Starch: Slight hydrolj'sis. 



Optimum temperature between 25° and 

 35° C. 



Source : Four isolates and 4 reisolates from 

 leaf spots of orchids by Ark and Thomas. 



Habitat: Pathogenic on Cattleya sp. and 

 Phalaenopsis sp. 



138. Pseudomonas dysoxyli Hutchin- 

 son, 1949. (New Zealand Jour. Sci. and 

 Tech., Sec. B, 30, 1949, 275.) 



dy.so'xy.li. M.L. neut.noun Dysoxylum 

 generic name of a forest tree; M.L. gen. noun 

 dysoxyli of Dysoxylum. 



Rods 0.4 to 0.6 by 0.6 to 1.0 micron. 

 Motile with 1 to 2 polar flagella. Gram-nega- 

 tive. 



Gelatin: No liquefaction. 



Beef -peptone agar colonies: Circular, 

 punctiform to 8 mm in diameter, gray, 

 translucent. Surface smooth and edges en- 

 tire to undulate. Medium brown in 1 week. 



Nutrient broth: Dense flocculent pellicle 

 at surface. Strong clouding in 3 days. 



Litmus milk: No change in 4 days. After 

 12 days amber whey at top and 1 inch of pink 

 precipitate at base. 



Indole not produced. 



Hydrogen sulfide not produced. 



Methyl red test negative; acetylmethyl- 

 carbinol not produced. 



Nitrites not produced from nitrates. 



Synthetic medium: Acid but no gas from 

 glucose, fructose, lactose, sucrose, raffinose 

 and mannitol within 3 weeks. No acid from 

 arabinose, maltose, melizitose, starch, 

 inulin, dextrin, glycerol or salicin. 



Starch hydrolj^zed. 



Temperature relations: Optimum, 25° C. 

 Minimum, 4° C. Maximum, 36° C. 



Source: Many cultures isolated from 

 diseased leaves of Dysoxylum spectabile in 

 New Zealand. 



Habitat: Pathogenic on leaves of the 

 forest tree Dysoxylum spectabile. 



139. Pseudomonas helianthi (Kawa- 

 mura, 1934) SSvulescu, 1947. {Bacterium 

 helianthi Kawamura, Ann. Phyt. Soc. 

 Japan, 4, 1934, 27; Savulescu, Anal. Acad. 

 Romane,III,^^, 1947, 11.) 



he.li.an'thi. Gr. heliiis the sun; Gr. 

 anthus a flower; M.L. mas. noun Helianthus 

 generic name of sunflower; M.L. gen. noun 

 helianthi of the sunflower. 



Rods 1.0 to 1.4 by 1.6 to 2.4 microns. 

 Motile with a single polar flagellum. Gram- 

 negative. 



Gelatin: No liquefaction. 



Beef agar colonies: White, circular, edges 

 entire. 



Broth: Turbid. Pellicle. 



Milk: Peptonized. Litmus reduced. 



Nitrates: Gas production. 



