150 



ORDER I. PSEUDOMONADALES 



Broth: Growth slow with moderate tur- 

 bidity in 48 hours. A thin pellicle. 



Milk: Alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3£, 1942,601). 



Acid but no gas from glucose, arabinose, 

 fructose and xylose. No acid from sucrose, 

 maltose, lactose, raffinose, glycerol or 

 mannitol. 



Starch partially digested. 



Temperature relations : Optimum between 

 22° and 30° C. Minimum, 1.5° C. Maximum 

 between 37° and 38° C. 



Chemical tolerance: Optimum pH be- 

 tween 6.0 and 6.6. Minimum, 5.0. Maximum 

 between 8.3 and 8.6. 



Source : Elliott used for her description 4 

 cultures isolated from lesions on sorgo, 

 sorghum and broom-corn. 



Habitat: Pathogenic on sorghum, Holcus 

 sorghum. 



143. Pseudomonas lignicola Westerdijk 

 and Buisman, 1929. (De lepenziekte, Arn- 

 hem, 1929, 51.) 



lig.ni'co.la. L. lignum wood; L. -cola 

 dweller; M.L. fem.noun lignicola wood 

 dweller or inhabitant. 



Rods. Single or short chains. Motile with 

 1 to several polar flagella. Gram-negative. 



Gelatin: No liquefaction. 



Malt agar streaks: Milk-w^hite with a 

 colorless edge. 



Broth: Turbid with light pellicle. 



Milk: No coagulation. No acid. 



Nitrites not produced from nitrates. 



Indole not produced. 



Starch hydrolysis slight. 



Optimum temperature, ±25° C. 



Source : From vessels of elm wood showing 

 dark discoloration, in Holland. 



Habitat: Pathogenic on elm wood. 



144. Pseudomonas petasitis (Takimoto, 



1927) Siivulescu, 1947. {Bacterium petasitis 

 (sic) Takimoto, Ann. Phyt. Soc. Japan, 

 2, 1927, 55; Savulescu, Anal. Acad. Romane, 

 III,^^, 1947, 11.) 



pe.ta'si.tis. Gr. mas. noun petasus a 

 sombrero, a broad-brimmed felt hat; Gr. 



mas. noun petasites a broad-leafed plant, 

 colt's foot; M.L. mas. noun Petasites generic 

 name; M.L. gen. noun petasitis of Petasites. 



Rods 0.8 to 1.1 by 1.1 to 1.7 microns. 

 Motile with a polar flagellum. Gram- 

 negative. 



Gelatin: No liquefaction. 



Beef agar colonies: White, circular or 

 amoeboid, butyrous. 



Broth: Strong turbidity. Pellicle. 



Milk: Coagulated in 30 days. 



Nitrites produced from nitrates with gas 

 formation. 



Indole not produced. 



Hydrogen sulfide not produced. 



No evident acid in peptone broth, but gas 

 from glucose, lactose and sucrose. Acid but 

 no gas from glycerol. 



Weak growth in broth plus 6 per cent salt. 



Temperature relations : Optimum between 

 27° and 30° C. Minimum, approximately 

 5° C. Maximum, 47° C. 



Source: Isolated from brown to black 

 lesions on Petasites japonicus in Japan. 



Habitat : Pathogenic on leaves of Petasites 

 japonicus. 



145. Pseudomonas woodsii (Erw. Smith, 

 1911) Stevens, 1925. {Bacterium woodsii 

 Erw. Smith, Bacteria in Relation to Plant 

 Diseases, 2, 1911, 62; Stevens, Plant Disease 

 Fungi, New York, 1925, 39.) 



wood 'si. i. Named for A. F. Woods, an 

 American plant pathologist; M.L. gen. noun 

 woodsii of Woods. 



Description from Burkholder and Guter- 

 man (Phytopath., 25, 1935, 118). 



Rods 0.67 by 1.56 microns. Motile with a 

 polar flagellum. Gram-negative. 



Gelatin: No liquefaction. 



Beef -extract agar slants : Growth slow and 

 scant, filiform, creamy, butyrous. 



Broth: Turbid. 



Milk: Becomes alkaline but otherwise 

 little changed. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3^, 1942,601). 



Acid but no gas from glucose, fructose, 

 galactose, arabinose, xylose, rhamnose, lac- 

 tose, glycerol and mannitol. Alkaline reac- 



