FAMILY IV. PSEUDOMONADACEAE 



161 



gested. Litmus reduced. Crystal formation 

 (Burkholder). 



Nitrites not produced from nitrates. 



Nitrogen sources utilized are peptone, 

 aspartic acid, alanine, leucine, sodium 

 ammonium phosphate, allantoin, t.yrosine, 

 uric acid and brucine. 



Indole not produced. 



Hydrogen sulfide not produced on lead 

 acetate agar. HoS produced after ZoBell 

 and Feltham's method (Burkholder). 



Selenium dioxide reduced. 



Lipolytic (Starr and Burkholder, ibid., 

 600). 



Acid but no gas from glucose, fructose, 

 galactose, lactose, sucrose, maltose, xylose, 

 raffinose, mannitol, glycerol and starch. 

 Alkali from salts of citric, lactic, malic and 

 succinic acids. Arabinose, rhamnose, dulci- 

 tol, salicin, inulin and cellulose not utilized. 



Starch hydrolj^zed. 



Pectate medium not liquefied. 



Temperature relations: Optimum, be- 

 tween 28° and 32° C. Minimum, between 5° 

 and 7° C. Maximum, 37° C. Thermal death 

 point between 53° and 55° C. 



pH range for growth: pH 5.2 to 10.5. Opti- 

 mum pH, between 6 and 8. 



Strict aerobe. 



Distinctive characters: Cultural charac- 

 ters the same or similar to those of Xantho- 

 monas juglandis. The two species do not 

 cross-infect. 



Source: 26 isolates from widely scattered 

 filbert orchards in Oregon and Washington. 



Habitat: Pathogenic on filberts {Corylus 

 avellana and C. maxima). 



15. Xanthomonas cucurbitae (Bryan, 

 1926) Dowson, 1939. (Bacterium cucurbitae 

 Bryan, Science, 63, 1926, 165; Bryan, Jour. 

 Agr. Res., 40, 1930, 389; Dowson, Zent. f. 

 Bakt., II Abt., 100, 1939, 190.) 



cu.cur'bi.tae. L. cucurbita a gourd; M.L. 

 fem.n. Cucurbita generic name; M.L. gen. 

 noun cucurbitae of Cucurbita. 



Rods 0.45 to 0.6 by 0.5 to 1.3 microns. 

 Motile, usually with a single polar flagellum. 

 Gram-negative. 



Gelatin: Liquefied. 



Beef -agar slants: Growth moderate, mus- 

 tard-yellow, undulating margins, viscid to 

 butyrous. 



Broth: Moderately turbid. Ring and j-el- 

 low sediment. 



Milk: Precipitation of casein; digestion. 

 Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide produced. 



Acid from glucose, galactose, fructose, 

 lactose, maltose, sucrose and glycerol. No 

 acid from mannitol. 



Starch hydrolyzed. 



Pectate medium not liquefied. 



Temperature relations: Optimum, be- 

 tween 25° and 30° C. Maximum, 35° C. 



pH range for growth: pH 5.8 to 9.0. Opti- 

 mum pH, between 6.5 and 7.0. 



Slight growth in 5 per cent salt. 



Aerobic. 



Source: Species first isolated from squash. 



Habitat : Causes a leaf spot of squash and 

 related plants. 



16. Xanthomonas desniodii Uppal and 

 Patel, 1949. (Uppal and Patel, in Patel, 

 Curr. Sci., 18, 1949, 213; also see Patel, 

 Indian Phytopath., 2, 1949, 5.) 



des.mo'di.i. M.L. neut.n. Desmodium 

 generic name of host; M.L. gen. noun 

 desmodii of Desmodium. 



Rods, 0.4 to 0.8 by 1.6 to 2.4 microns, oc- 

 curring singly or in pairs. Motile with a 

 single polar flagellum. Gram-negative. 



Gelatin: Liquefied. 



Nutrient agar slants: Growth fair, filiform, 

 flat, dull, smooth, opaque and pinard- 

 yellow. 



Potato-glucose agar (neutral) colonies : 

 Yellowish amber with colorless margins, 

 circular, viscid, smooth and wet. 



Beef broth: Growth slow. Moderate in 48 

 hours and good in 4 daj's. 



Milk: Litmus turns red in 10 days. Reduc- 

 tion slow. 



Indole not produced. 



Hydrogen sulfide production fair. 



Nitrites not produced from nitrates. 



No growth in Cohn's, Uschinsky's or 

 Fermi's solution. 



Acid but no gas from glucose, galactose, 

 lactose, mannitol, maltose and sucrose in 

 synthetic medium. Poor growth in salicin, 

 rafhnose, fructose, arabinose, xylose, dulci- 

 tol and glycerol, and no growth in tartaric, 



