FAMILY IV. PSEUDOMONADACEAE 



165 



rapid. Slightly raised, yellow, butyrous in 

 young cultures, difficult to pick up in old 

 cultures. 



Broth: Turbid in 24 hours. 



Milk: White curd in bottom. Litmus a 

 dirty wine color in supernatant liquid. 



Uschinsky's medium: Good growth. 



Fermi's solution: Scant growth. 



Indole not produced. 



Hydrogen sulfide not produced. 



Nitrites not produced from nitrates. 

 Ammonia produced from peptone. 



Acid but no gas from arabinose, glucose, 

 fructose, galactose, lactose, maltose, man- 

 nitol, raffinose, sucrose and xylose. Glycerol 

 not attacked. Tartrate utilized. 



Starch hydrolj^zed. 



Temperature relations: Optimum, be- 

 tween 22° and 28° C. Minimum, 10° C. Maxi- 

 mum, 37° C. Thermal death point, 50° C. 



Source: Six isolates from gardenia leaf 

 spots. 



Habitat : Causes a spot on young leaves of 

 gardenias. 



25. Xanthomonas nialvacearum (Erw. 

 f Smith, 1901) Dowson, 1939. (Pseudomonas 

 nialvacearum Erw. Smith, U. S. Dept. Agr., 

 Div. Veg. Phys. and Path., Bull. 28, 1901, 

 153; Dowson, Zent. f. Bakt., II Abt., 100, 

 1939, 190.) 



mal.va.ce.a'rum. L. malva the mallow; 

 M.L. fem.pl.n. Malvaceae the mallow family; 

 M.L. fem.pl. gen. n. malvacearum of the mal- 

 lows. 



Description from Elliott (Man. Bact. 

 Plant Pathogens, 1930, 153) and Lewis 

 (Phytopath., 20, 1930, 723). 



Rods. Motile with a single polar fiagellum. 

 Gram-negative. 



Gelatin: Liquefied. 



Agar slants: Growth moderate, convex, 

 smooth, glistening, pale yellow, wavy to 

 irregular margins. 



Broth: Slight to moderate turbidity. 

 Sediment. 



Milk: Casein precipitated and slowly di- 

 gested. 



Nitrites not produced from nitrates. 



Hydrogen sulfide produced (Burkholder) . 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 600). 



Acid but no gas from glucose, galactose. 



fructose, xylose, lactose, maltose, sucrose, 

 raffinose, glycerol, inulin and glycogen. 

 Alkaline reaction from salts of acetic, citric, 

 lactic and succinic acids. No fermentation 

 of arabinose, mannitol, dulcitol, salicin, and 

 salts of formic, oxalic and tartaric acids 

 (Lewis). 



Starch hydrolyzed (Lewis). 



Pectate medium not liquefied. 



Temperature relations: Optimum, be- 

 tween 25° and 30° C. Maximum, between 

 36" and 38° C. (Elliott). 



Source: Isolated from angular leaf spot 

 of cotton. 



Habitat: Pathogenic on cotton, where- 

 ever it is grown, causing a leaf spot, a stem 

 lesion and a boll lesion. 



26. Xanthomonas pelargonii (Brown, 

 1923) Starr and Burkholder, 1942. (Bac- 

 terium pelargoni (sic) Brown, Jour. Agr. 

 Res., 23, 1923, 372; Starr and Burkholder, 

 Phytopath., 32, 1942, 600.) 



pe.lar.go'ni.i. Gr. pelargus the stork: 

 M.L. neut.n. pelargonium generic name of 

 stork's bill; M.L. gen. noun pelargonii of 

 Pelargonium. 



Rods 0.67 by 1.02 microns. Encapsulated. 

 Motile with a single polar fiagellum. Gram- 

 negative. 



Gelatin: Slow liquefaction. 



Beef -agar colonies: Cream-colored, glis- 

 tening, round, with delicate internal mark- 

 ings. 



Broth: Turbid in 24 hours. Incomplete 

 pellicle. 



Milk: Alkaline. Clearing in bands. 



Nitrites not produced from nitrates. 



Indole production slight. 



Hydrogen sulfide produced. 



Lipolytic (Starr and Burkholder, Phy- 

 topath., 32, 1942, 600). 



Slight acid but no gas from glucose, su- 

 crose and glycerol. 



Starch hydrolysis feebly positive. 



Pectate medium liquefied. 



Temperature relations: Optimum, 27° C. 

 Maximum, 35° C. 



No growth in broth plus 3.5 per cent salt. 



Aerobic. 



Source: Isolated from spots on leaves of 

 Pelargonium from District of Columbia, 

 Maryland and New Jersey. 



