FAMILY IV. PSEUDOMONADACEAE 



181 



Agar slant: Yellow to orange, glistening, 

 translucent, slightly spreading. May lose 

 power to form pigment. 



Broth: Turbid, with yellow sediment. 



Litmus milk: Unchanged. 



Potato: Dark yellow, raised, rough, 

 spreading. 



Indole not produced. 



Nitrites and ammonia produced from 

 nitrates. 



Ammonia produced from peptone. 



Starch digested. 



Aerobic, facultative. 



Optimum temperature, 25° C. 



Habitat: Water and soil. 



7. Pseudonionas perlurida Kellerman 

 et al., 1913. (Kellerman, McBeth, Scales 

 and Smith, Cent, f . Bakt., II Abt., S9, 1913, 

 516; also see McBeth, Soil Sci., /, 1916, 

 472.) 



per.lu'ri.da. L. prefi.x per very; L. luridus 

 pale yellow, sallow; M.L. adj. perluridus 

 verj' sallow. 



Rods 0.4 by 1.0 micron. Motile with one 

 to three polar flagella. Gram-negative. 



Gelatin stab: Liquefied. 



Agar slant: Moderate, flat, faint yellow 

 growth. 



Broth: Turbid in 5 days. 



Litmus milk: Acid. Peptonization after 

 16 days. 



Potato: Scant, 3'ellow growth with 

 bleaching along line of growth. 



Indole not produced. 



Nitrites not produced from nitrates. 



Ammonia produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature, 20° C. 



Source: Soil from Virginia, Louisiana 

 and Missouri. 



Habitat: Soil. 



8. Pseudonionas ochracea (Zimmer- 

 mann, 1890) Chester, 1901. {Bacillus ochra- 

 ceus Zimmermann, Bakt. unserer Trink- 

 und Nutzwasser, Chemnitz, /, 1890, 60; 

 Chester, Man. Determ. Bact., 1901, 316.) 



och.ra'ce.a. Gr. noun ochra ochre; M.L. 

 adj. ochraceus of the color of ochre. 



Rods, 0.7 to 0.8 by 1.2 to 4.5 microns, 

 occurring in pairs and longer chains. Slow, 



undulatory motion (Zimmermann). Polar 

 flagella (Lehmann and Neumann, Bakt. 

 Diag., 1 Aufl., 2, 1896, 255). Gram-negative. 



Gelatin colonies: Pale yellow to golden, 

 ochre-yellow, slightly raised, with slightly 

 fringed margin, granular. 



Gelatin stab: Yellowish to yellow -gray 

 surface growth. Infundibuliform lique- 

 faction. Pale yellow to ochre-yellow sedi- 

 ment. 



Agar colonies: Thin, flat, yellowish, 

 smooth. 



Agar slant: Thin, yellowish gray to 

 ochraceous growth. 



Broth: Slightly turbid, with pale yellow 

 sediment. 



Litmus milk: Medium becomes slimv: 

 alkaline. 



Potato: Ochre-yellow streak. 



Indole produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide produced. 



Aerobic, facultative. 



Optimum temperature, 35° C. 



Source: Chemnitz tap water. 



Habitat: Water. 



9. Pseudonionas cerevisiae Fuhrmann, 

 1906. (Cent. f. Bakt., II Abt., 16, 1906, 309.) 



ce.re.vi'si.ae. L. cerevisia beer; M.L. 

 gen. noun cerevisiae of beer. 



Rods straight or slightly curved, 0.6 

 by 1.5 to 2.0 microns, occurring singly and 

 in chains. Motile, possessing a tuft of four 

 to six polar flagella. Gram-negative. 



Gelatin colonies: Circular, white, slightly 

 contoured, becoming brownish yellow. 



Gelatin stab: Slight yellowish growth in 

 stab. No liquefaction. 



Agar colonies: Thin, spreading, con- 

 toured. 



Agar slant: Moist, glistening, thin, pale 

 yellow, spreading, contoured. 



Litmus milk: Slow coagulation. 



Potato: Yellowish brown, spreading 

 growth. 



Indole not produced. 



Nitrites produced from nitrates. 



No gas from glucose. 



Aerobic, facultative. 



Optimum temperature, 30° C. 



Source: Isolated from beer. 



Habitat: Unknown. 



