FAMILY IV. PSEUDOMONADACEAE 



201 



1. Protaminobacter alboflavus den 



Dooren de Jong, 1926. (Thesis, Rotterdam, 

 1926, 159; also see Cent. f. Bakt., II Abt., 

 71, 1927, 218.) 



al.bo.fla'vus. L. adj. alhus white; L. 

 adj. flavus j-ellow; M.L. adj. alboflavus 

 whitish yellow. 



Rods. Non-motile. Gram-negative. 



Gelatin colonies: Circular, dry, light 

 yellow or colorless. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, opaque, pigment 

 bright red, yellow, light gray or colorless. 



Amine agar colonies: Circular, white to 

 dark yellow. 



See Table I for list of organic substances 

 utilized. 



Catalase produced. 



Aerobic, facultative. 



Optimum temperature, 30° C. 



Distinctive characters : The author recog- 

 nizes four varieties of this species which he 

 differentiates on the basis of organic sub- 

 stances attacked (see Table) and pigment 

 produced. Variety a shows light yellow 

 growth on gelatin, bright red on agar and 

 yellow on amine agar. Variety' /3 is light 3'el- 

 low on gelatin, yellow on agar and dark 

 yellow on amine agar. Variety y is light 

 yellow on gelatin, light gray on agar and 

 yellow on amine agar. Variety 5 is colorless 

 on gelatin and agar and white on amine 

 agar. 



Habitat: Soil and water. 



2. Protaminobacter ruber den Dooren 

 de Jong, 1926. (Thesis, Rotterdam, 1926, 

 159; also see Cent. f. Bakt., II Abt., 71, 1927, 

 218.) 



ru'ber. L. ruber red. 



Rods. Motile with a single polar flagellum 

 (Weaver, Samuels and Sherago, Jour. Bact., 

 35, 1938, 59). Gram-negative. 



Gelatin colonies: Circular, red, dry. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, red, opaque. 



Amine agar colonies: Circular, dark red. 



The following organic acids are attacked: 

 Acetic, lactic, |3-oxybutyric, glycerinic, 

 succinic, malonic, formic, methyl formic, 

 glutaric, maleinic, fumaric, malic, tartaric, 

 citric and quinic. 



The following amino compounds are 



Table I. — Organic Substances Utilized as a 



Source of Carbon by Varieties (biotypes) of 



Protaminobacter alboflavus 



Organic acids: 



Acetic 



Valerianic 



a-crotonic 



Undecyclic 



Lactic 



/3-oxybutyric 



Succinic 



Formic 



Glutaric 



Adipic 



Fumaric 



Malic 



Tartaric 



Citric 



/3-phenj'lpropionic 



Quinic 



Amino compounds: 



a-alanin 



a-aminocapronic acid 



Leucin 



Propionamid 



Capronamid 



Uric acid 



Hippuric acid 



Alcohol : 

 Ethyl 



Sugar : 

 Glucose 



Amines : 



Ethyl 



Diethyl 



Propyl 



Isopropyl 



Dipropyl 



Tripropyl 



Butyl 



Isobutyl 



Diisobutyl 



Amyl 



Diamyl 



Ethanol 



Glucosamin 



Benzyl 



