242 



ORDER I. PSEUDOMONADALES 



rounded ends, occurring singly, in pairs, 

 and at times in short chains. Motile. Polar 

 flagellate (Stanier, loc. cit.). Gram-negative. 



Fish-gelatin colonies: Punctiform, black, 

 glistening. 



Fish-gelatin stab: Slow, crateriform lique- 

 faction. 



Sea-weed agar colonies: Circular, flat, 

 opaque, glistening, white, slimy, entire. 

 Agar is dissolved. 



Fish-agar slant: Flat, white, elevated, 

 glistening, undulate growth. Liquefaction. 



Broth: Turbid with grayish white, slimy 

 sediment. 



Indole not produced. 



No action on sugars. 



Starch usuallj^ hydrolyzed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature, between 20° and 

 25° C. Minimum, between 0° and 5° C. Max- 

 imum, between 30° and 32° C. 



Source: Isolated from sea-water of the 

 Norwegian Coast. 



Habitat: Presumably found in sea water 

 and on sea weeds. 



22. Vibrio cyclosites Gray and Thorn- 

 ton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 

 92.) 



cyc.lo.si'tes. Gr. nonn ajclus a ring: Gr. 

 V. sited to eat; M.L. adj. cyclosites feeding 

 on rings, i.e., on ring compounds. 



Curved rods 0.5 to 1.0 by 1.5 to 4.0 mi- 

 crons. Motile by means of a single, polar 

 flagellum. Gram-negative. 



Gelatin colonies: Circular, buff to brown, 

 flat, smooth, glistening, entire. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular to irregular, pale 

 buff (later greenish), smooth, entire. 



Agar stab: Filiform, greenish buff, raised, 

 smooth, undulate. 



Broth: Turbid. 



Indole not reported. 



Acid from glucose. 



Starch not hydrolj'zed. 



Nitrites not produced from nitrates. 



Attacks phenol and ?«-cresol. 



Aerobic, facultative. 



Optimum temperature, between 30° and 

 35° C. 



Habitat: Soil. 



21. Vibrio neocistes Gray and Thorn- 

 ton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 

 92.) 



ne.o.cis'tes. Gr. adj. nensnev;; Gr. noun 

 ciste box; M.L. fern. gen. n. neocistes of New- 

 ark, a city. 



Curved rods 0.5 to 1.0 by 1.0 to 4.0 mi- 

 crons. Motile by means of one to three polar 

 flagella. Gram stain not recorded. 



Gelatin colonies: Liquefied. 



Gelatin stab: Liquefied. Medium red- 

 dened. 



Agar colonies: Circular or amoeboid, buff 

 to brownish, convex, smooth, glistening, 

 entire. 



Agar slant: Filiform, fluorescent, raised, 

 smooth, glistening, undulate. 



Broth: Turbid. 



Acid from glucose. 



Starch not hydrolyzed. 



Nitrites not produced from nitrates. 



Attacks naphthalene. 



Aerobic, facultative. 



(Optimum temperature, between 30° and 

 35° C. 



Habitat: Soil. 



23. Vibrio oxaliticus Bhat and Barker, 

 1948. (Jour. Bact., 55, 1948, 359.) 



ox.a.li'ti .cus. Gr. noun oxalis sorrel, a 

 sour plant; Gr. adj. lyticus dissolving; M.L. 

 adj. oxaliticus intended to mean decom- 

 posing oxalate. 



Curved rods 0.4 by 1.3 microns. Actively 

 motile by means of a single, polar flagellum. 

 Not encapsulated. Gram-negative. 



Nutrient agar colonies: Small, moist, 

 raised, entire; no chromogenesis. Pin-point 

 in size in 48 hours, growing slowly until 

 they reach a diameter of 1.5 mm in 6 days. 



Nutrient broth: Moderate growth after 

 24 hours, appearing at first as a thin film 

 while a slight, general turbidity develops 

 in another 24 to 48 hours. 



Calcium oxalate agar: Growth rapid and 

 colonies small; medium becomes alkaline. 



Oxalate broth: Becomes turbid following 

 the formation of a slight surface film. 



Oxalates and pyruvates support growth 

 within 3 to 4 days when added to a mineral 

 medium as the sole carbon source; formates 

 support growth only when the incubation 

 period is extended. The following do not 



