308 



ORDER IV. EUBACTERIALES 



Ammonium chloride and the 19 amino 

 acids which were tested may serve as 

 sources of nitrogen; the amino acids may 

 also be utilized as carbon sources (Campbell 

 and Williams, op. cit., 1951a, 506). 



Nitrites not produced from nitrates. 



Trimethylamine not produced from tri- 

 methylamine oxide, betaine, choline or 

 acetyl choline (Campbell and Williams, 

 Jour. Bact., 62, 1951a, 250). 



Inorganic sulfur may serve as a source of 

 sulfur (Campbell and Williams, loc. cit.). 



Aerobic, facultative. 



Optimum temperature, 25° C. 



Habitat: Milk. 



13. Achromobacter eurydice (White, 

 1912) Bergey et al., 1925. {Bacterium eury- 

 dice White, U. S. Dept. of Agr., Bur. of 

 Entomol., Circ. 157, 1912, 3, and U. S. 

 Dept. of Agr. Bull. 810, 1920, 15; Bergey et 

 al., Manual, 2nd ed., 1925, 170; Acineto- 

 bacter eurydice Brisou and Pr^vot, Ann. 

 Inst. Past., 86, 1954, 727.) 



eu.ry'di.ce. Gr. fem.n. Eurydice the wife 

 of Orpheus. 



Small, slender rods with slightly rounded 

 ends; occur singly and in pairs. Non-motile. 

 Gram-negative. 



Gelatin stab : A bluish gray growth occurs 

 along the line of inoculation. No liquefac- 

 tion. 



Glucose agar colonies: Bluish gray, cir- 

 cular, smooth glistening, entire. 



Broth: Uniform turbidity with viscid 

 sediment. 



Potato: Slight, grayish growth. 



Litmus milk: Unchanged. 



Acid from glucose, but little or no action 

 on other carbohydrates. 



Of 19 amino acids tested, none was re- 

 quired for growth; preformed growth fac- 

 tors also were not required (Campbell and 

 Williams, Food Research, 16, 1951a, 506). 



Ammonium chloride and the 19 amino 

 acids which were tested may serve as sources 

 of nitrogen; the amino acids may also be 

 utilized as carbon sources (Campbell and 

 Williams, loc. cit.). 



Trimethylamine not produced from tri- 

 methylamine oxide, betaine, choline or 

 acetyl choline (Campbell and Williams, 

 Jour. Bact., 6.?, 1951b, 250). 



Inorganic sulfur may serve as a source of 

 sulfur (Campbell and Williams, op. cit., 

 1951a, 506). 



Aerobic, facultative. 



Innocuous when fed to bees. Not patho- 

 genic when inoculated subcutaneously in 

 rabbits. 



Source: Occurs as a secondary invader 

 in European foulbrood of bees. 



Habitat: Unknown. 



14. Achromobacter delmarvae Smart, 

 1932. (Jour. Bact., 23, 1932, 41; also see 

 Jour. Agr. Research, 51, 1935, 363; Acineto- 

 bacter delmarvae Brisou and Prdvot, Ann. 

 Inst. Past., 86, 1954, 727.) 



del.mar'vae. M.L. gen. noun delmarvae of 

 Delmarva; named for Delmarva, the name 

 given to an area comprising portions of the 

 states of Delaware, Maryland and Virginia. 



Short rods which average 0.75 by 1.5 

 microns; rounded ends; occur singly, in 

 pairs and in short chains. Non-motile. 

 Gram-negative. 



Gelatin colonies: Similar to agar colonies. 



Gelatin stab: Scant growth. No lique- 

 faction. 



Beef -infusion agar colonies: Small, cir- 

 cular, raised, glistening, translucent, blu- 

 ish white, amorphous; margin entire; edges 

 smooth. 



Agar stab: Abundant growth. Surface 

 growth round, smooth, glistening, bluish 

 white, raised. Filiform growth the whole 

 length of stab, but growth best at top. 



Agar slant: Abundant, filiform, raised, 

 glistening, smooth, translucent, bluish 

 white growth; no odor; old cultures slightly 

 viscid. Medium unchanged. 



Nutrient broth: Turbid. Delicate, white 

 pellicle. Sediment abundant, white, slightly 

 stringy. No odor. Color of medium un- 

 changed. 



Sterile milk: Slow growth. No peptoniza- 

 tion. Coagulation in 12 to 14 days. Milk 

 turns chocolate-brown beginning at top. 



Litmus milk: Acid, with reduction of 

 litmus in 5 days. Coagulation, with return 

 of pink color in 12 to 14 days. Browning of 

 medium. 



Potato: Abundant, grayish white, glisten- 

 ing, smooth, raised growth. Medium changes 

 from white to smoke-gray. 



