FAMILY III. ACHKOMOBACTERACEAE 311 



2. Marine forms which have been described from growth in media prepared with 

 sea water.* 

 a. Acid from glucose in nutrient broth. 



b. Yellow growth on nutrient agar; orange growth on gelatin. 



16. Flavobacterium halohydrium. 



bb. Buff to yellow growth on nutrient agar; faint yellow growth on gelatin. 



17. Flavobacterium neptunium. 

 aa. No acid from glucose in nutrient broth. 



b. Nitrites produced from nitrates. 



18. Flavobacterium okeanokoites . 

 bb. Nitrites not produced from nitrates. 



19. Flavobacterium marinovirosum. 



B. Produce a pigmentation which shows no pronounced change under various cultural 

 conditions. 



1. Fresh-water forms or marine forms described from growth in media prepared with 

 fresh water.* 



a. Litmus milk modified. 



b. Litmus milk peptonized and becomes alkaline. Gelatin liquefied, 

 c. Indole produced. Pigmentation yellow. 



20. Flavobacteriiim suaveolens. 

 cc. Indole not produced. Pigmentation amber-3-ellow. 



21. Flavobacterium marinum* 

 bb. Litmus milk becomes slightly acid. Gelatin not liquefied. 



22. Flavobacterium lactis. 

 aa. Litmus milk not modified. 



b. Gelatin liquefied. 



23. Flavobacterium devorans. 

 bb. Gelatin not liquefied. 



24. Flavobacterium invisibile. 



2. Marine forms which have been described from growth in media prepared with 

 sea water. 



a. Grows in litmus milk. Pigmentation yellow. 



25. Flavobacterium marinotypicum. 

 aa. Does not grow in litmus milk. Pigmentation bright orange. 



26. Flavobacterium piscicida. 



1. Flavobacterium aquatile (Frank- Rods, 0.5 to 0.7 by 1.0 to 3.0 microns, 



land and Frankland, 1889) Bergey et al., approaching coccobacillary form in young 



1923. (Bacillus aguatilis G. and P. Frank- cultures; filamentous forms, 10 to 40 microns 



land, Ztschr. f. Hyg., 6, 1889, 381; Flavobac- long, occur in liquid or on solid media. Non- 



terium aquatilis (sic) Bergej" et al.. Manual, motile. Gram-negative. 



1st ed., 1923, 100.) Gelatin: Giant colonies irregular, mucoid 



a.qua'ti.le. L. adj. aquatilis living in and uniformly raised with a conspicuous 



water. center. Yellow, becoming orange. Limited 



Description prepared by Prof. Owen B. liquefaction. 



Weeks (see Jour. Bact., 69, 1955, 649) from Agar colonies: Smooth, 1 to 3 mm in 



a study of Culture F36 (ATCC 11947) diameter, entire, glistening, transparent, 



isolated from water by Dr. E. Windle light yellow becoming brownish yellow. At 



Taylor, Metropolitan Water Board, Lon- 10° C., or when there is abundant growth, 



don, England, from the same deep wells in red components are most conspicuous, 



chalk that were studied by the Franklands. Where sucrose is added to the agar, the 



* See footnote on preceding page. 



