316 



ORDER IV. EUBACTERIALES 



Broth: Turbid with white sediment. 



Blood serum: Light graj^-colored growth 

 in 2 to 3 days. 



Litmus milk: Action not recorded. 



Potato: No growth. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 35° C. 



Pathogenic for laboratory animals. 



Source : Found constantly in Berlin drain 

 water. 



Habitat: Sewage. 



11. Flavobacterium halmephiluin Ela- 

 zari-Volcani, 1940. (Studies on the Micro- 

 flora of the Dead Sea. Thesis. Hebrew 

 Univ., Jerusalem, 1940, VIII and 85.) 



hal.me'phi.lum. Gr. noun halme brine, 

 sea water; Gr. adj. philus loving; M.L. adj. 

 halinephilus sea-water-loving. 



Rods, 0.5 to 0.6 by 0.7 to 2.0 microns, 

 occurring singly and in pairs; morphology 

 and size unchanged as salt concentrations 

 vary. Non-motile. Gram-negative. 



Gelatin stab (12 per cent salt-1 per cent 

 proteose peptone-15 per cent gelatin) : 

 Scant growth; no liquefaction after a 

 month. 



Agar colonies (12 per cent salt-1 per cent 

 proteose peptone-2 per cent KNO3) : Circu- 

 lar, smooth, entire, convex, glistening, 

 opaque, yellowish. 



Agar slant (12 per cent salt-1 per cent 

 proteose peptone-2 per cent KNO3) : Moder- 

 ate, hliform, raised, smooth or slightly 

 rugose, opaque, j^ellowish growth. 



Broth (12 per cent salt-1 per cent pep- 

 tone) : Turbid; delicate pellicle or ring and 

 granular, flaky sediment; broth turns 

 yellowish. 



Indole not produced. 



No acid or gas from glucose, fructose, 

 galactose, mannose, lactose, sucrose, mal- 

 tose, arabinose, xylose, raffinose, inulin, 

 dextrin, glycerol, mannitol or salicin. 



Starch not hydrolyzed. 



Nitrites produced from nitrates in trace 

 amounts. 



Aerobic. 



Optimum temperature, 30° C. 



Salt tolerance: Halotolerant, not grow- 

 ing in 0.5 per cent salt, but growing strongly 

 in 3 to 24 per cent and moderately in 30 per 

 cent salt and in Dead Sea water. 



Source: Isolated from the water of the 

 Dead Sea. 



Habitat: Found in places where the salt 

 content of water is high. 



12. Flavobacteriiuii rhenaniim (Mi- 

 gula, 1900) Bergey et al., 1948. (Burri's 

 Rhine water bacillus, Frankland and Frank- 

 land, Microorganisms in Water, 1894, 483; 

 Bacillus rhenanus Migula, Syst. d. Bakt., 

 2, 1900, 713; Flavobacterium rhenanus (sic) 

 Bergey et al.. Manual, 6th ed., 1948, 433.) 



rhe.na'num. L. adj. rhenanus pertaining 

 to the Rhine. 



Original description supplemented by 

 Bergey {loc. cit.) from his private notes as 

 indicated; Steinhaus (Jour. Bact., ^2, 1941, 

 762 and 772) apparently found the same 

 organism and has added other characters. 



Rods, 0.7 by 2.5 to 3.5 microns, with 

 rounded ends, occurring singly and in chains 

 (Burri). Motile, possessing peritrichous 

 flagella (Bergey). Gram-negative (Bergey). 



Gelatin colonies: Convex, colorless, 

 transparent, becoming yellowish. 



Gelatin stab: Infundibuliform liquefac- 

 tion. 



Agar colonies: Small, smooth, convex, 

 entire. 



Agar slant : Bright yellow growth (Stein- 

 haus) . 



Glycerol agar slant: Thin, shining, honey- 

 colored. Growth dry and tough. 



Broth: Turbid, with orange-colored pel- 

 licle and sediment. 



Litmus milk: Soft coagulum, becoming 

 slightly alkaline with yellow ring. 



Potato: Moist, glistening, thin, flat, 

 orange to rust-colored growth. 



Indole not produced (Bergey) . 



Hydrogen sulfide not produced (Stein- 

 haus) . 



Acid from glucose, maltose and sucrose 

 but not from lactose (Steinhaus). 



Starch not hydrolyzed (Steinhaus) . 



Nitrites produced from nitrates (Bergey). 



Aerobic, facultatively anaerobic. 



Optimum temperature, 30° C. 



Source : Isolated from Rhine River water 

 (Burri). From water (Bergey). From eggs 

 in the ovary of a walking stick {Diaphero- 

 mera femorata Say) (Steinhaus) . 



