FAMILY III. ACHROMOBACTERACEAE 



317 



Habitat: Presumably widely distributed 

 in nature. 



13. Flavobacteriuni harrisonii Bergey 

 et al., 1923. (Variety No. 6, Harrison, Rev. 

 gen. du Lait, 5, 1905, 129; Bacillus lactis 

 harrisonii Conn, Esten and Stocking, Ann. 

 Rept. Storrs Agr. Exp. Sta., 1906, 169; 

 Bergey et al., Manual, 1st ed., 1923, 104.) 



har.ri.so'ni.i. M.L. gen. noun harrisonii 

 of Harrison; named for Prof. F. C. Harrison, 

 the Canadian bacteriologist who first 

 isolated this species. 



Rods, 0.25 to 0.75 by 0.3 to 3.5 microns, 

 occurring singly and occasionally in short 

 chains. Motile by means of peritrichous 

 flagella. Gram-negative. 



Gelatin colonies: Small, gray, glistening, 

 lobular, citron-yellow, slimy. 



Gelatin stab: Villous growth in stab. 

 Slow crateriform to napiform liquefaction. 



Agar slant: Growth luxuriant, viscous, 

 spreading, becoming dirty to brownish 

 citron-yellow. 



Broth: Turbid, with viscid ring and 

 gelatinous sediment; sweetish odor; alka- 

 line. 



Litmus milk: Colorless to gray and slimy, 

 becoming yellow, alkaline. 



Potato: Luxuriant, yellow, spreading, 

 slimy growth. 



Indole not produced. 



Glucose, lactose, maltose and sucrose 

 broths turn alkaline with a disagreeable 

 odor. Reaction of glycerol broth remains 

 neutral. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 25° C. 



Source: Isolated from slimy milk. 



Habitat: Unknown. 



14. Flavobacteriuni diffusuni (Frank- 

 land and Frankland, 1889) Bergey et al., 

 1923. (Bacillus diffusus G. and P. Frankland, 

 Ztschr. f. Hyg., 6, 1889, 396; Bergey et al., 

 Manual, 1st ed., 1923, 100.) 



dif.fu'sum. L. adj. diffustis spreading, 

 diffuse. 



Original description supplemented by 

 Harrison (Canadian Jour. Res., 1, 1929, 233) 

 as indicated. 



Rods, 0.5 by 1.5 microns, occurring singly 



and in chains. Motile, possessing peritrich- 

 ous flagella. Gram-negative (Harrison). 



Gelatin colonies: Thin, bluish green, 

 spreading, later faint yellow. 



Gelatin stab: Thin, glistening, yellowish 

 green surface growth. Slow, crateriform 

 liquefaction. 



Agar slant: Thin, light j-ellow, glistening 

 growth (Frankland and Frankland); capu- 

 cine-buff (yellow-red-yellow) growth (Har- 

 rison) . 



Broth: Turbid, with greenish yellow sedi- 

 ment. 



Litmus milk: Unchanged (Harrison). 



Potato: Thin, smooth, greenish j^ellow, 

 glistening growth. 



Indole not produced (Harrison). 



Slight acid from glucose. No acid from 

 sucrose or lactose (Harrison). 



Nitrites produced from nitrates (Harri- 

 son). 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 25° and 

 30° C. 



Source: Originally found in soil. Found 

 also by Tataroff (Die Dorpater Wasserbak- 

 terien, Dorpat, 1891, 58) in fresh water and 

 by Harrison from the skin of halibut from 

 both the Atlantic and Pacific shores of 

 Canada. 



Habitat: Soil, fresh and sea waters. 



15. Flavobacteriuni rigense Bergej' et 

 al., 1923. (Bacillus brunneus rigensis Baza- 

 rewski. Cent. f. Bakt., II Abt., 15, 1905, 1; 

 Bergey et al., Manual, 1st ed., 1923, 100.) 



ri.gen'se. M.L. adj. rigensis pertaining to 

 Riga; named for Riga, the city where this 

 species was isolated. 



Rods, 0.75 by 1.7 to 2.5 microns, occurring 

 singly. Motile by means of peritrichous 

 flagella. Gram-negative. 



Gelatin colonies: Circular, entire to undu- 

 late, grayish white, homogeneous. 



Gelatin stab: Smooth, j'ellowish surface 

 growth. Infundibuliform liquefaction. 

 Brownish yellow sediment. 



Agar slant: Narrow, whitish streak, be- 

 coming yellowish brown, spreading. Pig- 

 ment is water- and alcohol -soluble but 

 insoluble in ether. 



Broth: Turbid with pellicle and brownish 

 sediment. Cells encapsulated. 



