320 



ORDER IV. EUBACTERIALES 



Gelatin stab: Rapid, stratiform liquefac- 

 tion. Medium becomes brown. 



Agar colonies: Small, circular, smooth, 

 yellow, amorphous, undulate margin. 



Agar slant: Moderate, flat, glistening, 

 opaque, butyrous, yellow, with aromatic 

 odor. 



Broth: Turbid with scant sediment. 

 Aromatic odor, becoming cheesy. 



Litmus milk: Peptonized. Alkaline. 



Potato: Abundant, yellow, glistening 

 growth becoming brown. 



Indole produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide produced. 



Slight acid but no gas from glucose, su- 

 crose and glycerol. No acid from lactose. 



Starch is hydrolyzed. 



Blood serum is liquefied. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 25° C. 



Source: Isolated from dairy wastes. 



Habitat: Unknown. 



21. Flavobacterium niarinum Har- 

 rison, 1929. (Canadian Jour, of Research, 

 1, 1929,234.) 



ma.ri'num. L. adj. marinus marine, of the 

 sea. 



Rods, 0.8 by 1.2 to 1.3 microns, with 

 rounded ends, occurring singly and in pairs. 

 Motile by means of 4 to 5 peritrichous 

 flagella. Encapsulated. Gram-variable; 

 show blue granules in Gram-negative rods. 



Gelatin colonies: Circular, iridescent, 

 whitish margin with pale yellow center. 

 Liquefaction. 



Gelatin stab: Saccate to stratiform lique- 

 faction. 



Agar colonies: Circular, pale yellow, 

 smooth, convex, granular; reticulate edge. 



Agar slant: Amber-j^ellow, slightly raised, 

 spreading, smooth, glistening, transparent 

 growth. 



Ammonium phosphate agar: Scant 

 growth. 



Broth: Turbid, sediment. 



Litmus milk: Alkaline. Digestion without 

 coagulation. Clear serum. 



Potato: Abundant, amber-yellow, becom- 

 ing dirty yellow, spreading, glistening 

 growth. 



Indole not produced. 



Hydrogen sulfide not produced. 



Faint acidity from glucose; no action on 

 lactose or sucrose. 



Nitrites not produced from nitrates. 

 Trace of ammonia formed. 



Loeffler's blood serum not liquefied. Faint 

 yellow, spreading growth. 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 20° and 

 25° C. 



Source: Isolated from living halibut ob- 

 tained at 30 to 50 fathoms. Pacific Ocean. 

 Gibbons (Contrib. to Canadian Biol, and 

 Fish., 8, 1934, 279) reports this species as 

 occurring in the slime and feces of cod 

 (Gadus callarias), halibut {Hippoglossus 

 hippoglossus) and skate {Raja erinacea). 



Habitat: Skin and feces of fishes. 



22. Flavobacterium lactis Bergey et al., 

 1923. {Bacillus aromaticus lactis Grimm, 

 Cent. f. Bakt., II Abt., 8, 1902, 584; Bergey 

 et al.. Manual, 1st ed., 1923, 108.) 



lac'tis. L. noun lac milk; L. gen. noun 

 lactis of milk. 



Rods, 0.7 to 1.0 by 3.5 to 4.0 microns, oc- 

 curring singly, in pairs and in chains. Motile 

 by means of peritrichous flagella. Gram- 

 negative. 



Gelatin colonies: Circular, light j-ellow, 

 slimy, concentrically ringed, undulate. 



Gelatin stab: Slimy surface growth. No 

 liquefaction. 



Agar slant: Slimy, j-ellowish, smooth, 

 moist. 



Broth: Turbid, with abundant sediment. 



Litmus milk: Slightly acid. 



Potato: Growth thick, slimy, brownish, 

 with yellowish margin. 



Indole not produced. 



Nitrites not produced from nitrates. 



Cultures have pleasant odor. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 25° C. 



Source: Isolated from milk. 



Habitat: Unknown. 



23. Flavobacterium tlevorans (Zimmer- 

 mann, 1890) Bergey et al., 1923. {Bacillus 

 devorans Zimmermann, Bakt. unserer Trink- 

 u. Nutzwasser, Chemnitz, 1, 1890, 48; 

 Bergey et al., Manual, Ist ed., 1923, 102.) 



