FAMILY III. ACHROMOBACTERACEAE 



325 



Source: Isolated from fronds of A''ereoc?/s- Source: Isolated from Ncreocystis luet- 



tis luetkeana. keana. 



Habitat: Found on marine algae. Habitat: Found on marine algae. 



4. Agarbacteriuni reducans Angst, 

 1929. (Puget Sound Biol. Sta. Pub., 7, 1929, 



57.) 



re.du'cans. L. part. adj. reducmis bring- 

 ing back, reducing. 



Short rods, 0.6 by 0.8 micron, with 

 rounded ends, occurring singly and in pairs. 

 Not encapsulated. Motile. Gram-negative. 



All media contained a decoction made by 

 boiling or autoclaving fronds of Iridaea, 

 a seaweed, in sea water or a 3 per cent salt 

 solution. 



Fish gelatin colonies: Circular, sunken, 

 entire, crateriform, granular; growth is 

 rapid. 



Fish gelatin slant: Abundant, filiform, 

 sunken, glistening, smooth, opaque, buff, 

 butyrous growth. 



Fish gelatin stab: Crateriform liquefac- 

 tion; growth best at top. 



Agar colonies: Circular, smooth, flat, 

 entire, granular; growth is moderate. 



Agar slant: Abundant, filiform, flat, glis- 

 tening, smooth, opaque, buff, butj^rous 

 growth. 



Fish agar slant: Abundant, filiform, flat, 

 glistening, smooth, opaque, white, butyrous 

 growth. 



Meat agar slant: Abundant, echinulate, 

 flat, glistening, smooth, opaque, white, 

 butyrous growth. 



Broth: Turbid, no sediment; no pellicle. 



Plain milk unchanged. 



Potato: No growth. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from sucrose, rhamnose, arabinose 

 and mannitol. No acid from lactose or 

 xylose. 



Starch is hydrolyzed. 



Agar is digested; cellulose not attacked. 



Aerobic. 



Methylene blue reduced. 



Temperature relations: Optimum, be- 

 tween 25° and 28° C. Minimum, less than 5° 

 C. Maximum, between 36° and 45° C. 



5. Agarbacteriuni amocontactum (Zo- 

 Bell and Allen, 1935) Breed, comb. nov. 

 (Flavobacterium amocontactum ZoBell and 

 Allen, Jour. Bact., 29, 1935, 246.) 



a.mo.con.tac'tum. L. v. amo to like, love; 

 L. noun contactus contact; M.L. adj. amo- 

 contactus presumably means contact-loving. 



Slender rods, 0.4 to 0.7 by 1.6 to 2.3 mi- 

 crons, with rounded ends, occurring singly 

 and in irregular clumps. Encapsulated. 

 Activel}^ motile by means of peritrichous 

 flagella. Stain very lightly. Gram-negative. 



Gelatin stab: Good, filiform growth with 

 rapid, saccate liquefaction. 



Agar colonies: Circular, 2 to 4 mm in di- 

 ameter, 3'ellow. 



Agar slant: Abundant, filiform, smooth, 

 glistening, bright yellow growth with a 

 butyrous consistency. Originally agar was 

 liquefied, but this property was lost follow- 

 ing artificial cultivation. 



Sea-water broth: Good growth with ring 

 at surface. Strongly turbid; abundant, vis- 

 cid sediment; no odor. 



Milk: No growth. 



Potato: No growth. 



Potato dialyzed in sea water: Slight, yel- 

 low growth. 



Indole not produced. 



Hydrogen sulfide produced. 



No acid from glucose, lactose, sucrose, 

 .xylose or mannitol. 



Starch not hydrolyzed. 



Agar is digestea. 



Nitrites produced from nitrates. 



Ammonia produced from peptone. 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 18° and 

 21° C. 



Optimum reaction, pH 8.0. 



Distinctive character: Adheres firmly to 

 submerged glass slides and cannot be re- 

 moved with running water. 



Source: Many cultures were isolated from 

 glass slides submerged in sea water. 



Habitat: Sea water. 



6. Agarbacteriuni pastinalor (Gore- 



