FAMILY III. ACHROMOBACTERACEAE 



327 



8. Agarbacteriuni bufo Angst, 1929. 

 (Puget Sound Biol. Sta. Pub., 7, 1929, 53.) 



bu'fo. L. noun b^lfo the toad. 



Short rods, 0.6 by 0.8 micron, with 

 rounded ends, occurring singly and in pairs. 

 Not encapsulated. Non-motile. Gram- 

 negative. 



All media contained a decoction made 

 by boiling or autoclaving fronds of Iridaea, 

 a seaweed, in sea water or a 3 per cent salt 

 solution. 



Fish gelatin colonies: Circular, sunken, 

 crateriform, granular, yellow; growth is 

 slow. 



Fish gelatin slant: Scant, filiform, flat, 

 glistening, smooth, translucent, white to 

 yellowish, butj-rous growth. 



Fish gelatin stab : Stratiform liquefaction ; 

 growth best at top. 



Agar colonies: Circular, concentrically 

 ringed, sunken, entire, granular, yellow; 

 growth is slow. 



Agar slant: Abundant, filiform, raised, 

 glistening, contoured, opaque, j^ellow, mem- 

 branous growth. 



Fish agar slant: Abundant, filiform, flat, 

 glistening, smooth, opaque, yellow to 

 orange, butyrous growth. 



Meat agar slant: Abundant, filiform, flat, 

 glistening, contoured, opaque, yellow to 

 orange, butyrous growth surrounded by a 

 white halo. 



Broth: Moderately turbid; scant sedi- 

 ment; granular, membranous pellicle. 



Plain milk unchanged; surface growth 

 yellow. 



Potato: No growth. 



Indole not produced. 



Hydrogen sulfide not produced. 



Slight acid from mannitol. No acid from 

 glucose, sucrose, lactose, xylose, rhamnose 

 or arabinose. 



Starch not hydrolyzed. 



Agar digested; cellulose not attacked. 



Nitrites produced from nitrates. 



Aerobic. 



Methylene blue reduced. 



Temperature relations: Optimum, be- 

 tween 25° and 28° C. Minimum, between 5° 

 and 10° C. Maximum, less than 36° C. 



Source: Isolated from Odonthalia kam- 

 tschatica. 



Habitat: Found on marine algae. 



9. Agarbacteriuni polysiphoniae 



(Lundestad, 1928) Breed, comb. nov. {Bac- 

 terium polysiphoniae Lundestad, Cent. f. 

 Bakt., II Abt., 75, 1928, 331.) 



po.ly.si.pho'ni.ae. M.L. noun Polysi- 

 phonia a genus of red algae; M.L. gen. noun 

 polysiphoniae of Polysiphonia. 



Rods, 0.5 to 0.6 by 2.0 to 4.0 microns, with 

 rounded ends, occurring singly. Non-motile. 

 Gram-negative. 



Fish gelatin colonies: Circular, slightl}- 

 glistening, bright .yellow, transparent, with 

 dense center. 



Fish gelatin stab : Slight, yellowish growth 

 on surface. Slow, saccate liquefaction. 



Seaweed agar colonies : Circular, flat, light 

 yellow, with concentric rings and diffuse 

 margin. Agar is disintegrated. 



Fish agar slant : Yellow, flat growth with 

 undulate margin. 



Broth: Turbid; flocculent pellicle; 5'ellow- 

 ish sediment. 



Indole not produced. 



Carbohydrates not attacked. 



Starch slightly hydrolyzed. 



Nitrites not produced from nitrates. 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, 25° C. 

 Minimum, between 5° and 10° C. Maximum, 

 between 27° and 30° C. 



Source: Isolated from sea water from the 

 Norwegian Coast. 



Habitat: Sea water. 



10. Agarbacteriuni ceramicola (Lund- 

 estad, 1928) Breed, comb. nov. (Bacterium 

 ceramicola Lundestad, Cent. f. Bakt., II 

 Abt., 75, 1928, 332.) 



ce.ram.i'co.la. M.L. noun Ceramium a 

 genus of red algae; L. v. colo to dwell; M.L. 

 fem.n. ceramicola the Ceramium dweller. 



Rods, 0.5 to 0.6 by 1.4 to 2.4 microns, with 

 rounded ends, occurring singly and Ij'ing 

 side by side. Non-motile. Gram-negative. 



Fish gelatin colonies: Circular, glistening, 

 transparent, yellow. 



Fish gelatin stab: Slight, yellow surface 

 growth. Crateriform liquefaction. 



Sea-water agar colonies: Circular, flat, 

 transparent, glistening, light yellow; difi"use 

 margin. Agar is disintegrated. 



Fish agar slant: Moderate, yellow, flat, 

 entire, glistening, opaque growth. 



