FAMILY III. ACHROMOBACTERACEAE 



331 



ate, propionate, salic3'late, valerate, oxal- 

 ate, butyrate, mandelate, benzoate (0.5 

 I)er cent), ethanol, methanol, iso-amyl 

 alcohol, n-amyl alcohol, tertiary bntanol, 

 n-propanol and luniichrome are not utilized 

 as carbon sources. 



Starch is hN'drolj^zed. 



Chitin is hydrolyzed. 



Lipolytic. 



Nitrites produced from nitrates. 



Ammonia produced from peptone. 



Urease-negative. 



Casein is hj'drolj-zed. 



Trimethylamine not produced from tri- 

 methylamine oxide, choline or betaine. 



Growth not inhibited bj' 10 per cent NaCl. 



Aerobic, facultative. 



Optimum temperature, between 20° and 

 30° C. Growth at 4° C. 



Source: Isolated from marine mud. 



Habitat : Found in sea water. 



5. Beneckea indolthetica (Campbell 

 and Williams, 1951) Campbell, comb. nov. 

 (Flavobacterium indoltheticum Campbell and 

 Williams, Jour. Gen. Microbiol., 5, 1951, 

 894.) 



in.dol.the'ti.ca. M.L. noun indolum in- 

 dole; Gr. adj. theticus positive; M.L. adj. 

 indoltheticus indole-positive. 



Rods, 0.4 to 0.9 by 1.0 to 1.5 microns, 

 occurring singly, in pairs and in occasional 

 clumps. Motile by means of peritrichous 

 flagella. Gram-negative. 



Gelatin stab: Crateriform liquefaction. 



Agar colonies: Circular, smooth, entire, 

 opaque, yellow to yellow-orange. 



Agar slant: Yellow to yellow-orange, 

 smooth, opaque growth. 



Broth: Turbid; slight surface scum; 

 yellow to orange sediment. 



Litmus milk: Acid in 2 days. 



Indole is produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, fructose, 

 sucrose, maltose, dextrin, mannose, raffi- 

 nose, trehalose and cellobiose. No acid from 

 lactose, arabinose, rhamnose, xylose, inulin, 

 mannitol, salicin, dulcitol, inositol, melezi- 

 tose or adonitol. 



Glucose, fructose, sucrose, maltose, dex- 

 trin, mannose, raffinose, salicin, cellobiose, 

 trehalose, pyruvate (0.3 per cent), acetate. 



chitin, starch, glycogen and glucosamine 

 are utilized as carbon sources. Lactose, 

 arabinose, rhamnose, xylose, inulin, man- 

 nitol, dulcitol, galactose, inositol, melezi- 

 tose, adonitol, succinate, malate, fumarate 

 (0.5 per cent), lactate, malonate, tartrate, 

 citrate, levulinate, |3-alanine, asparaginate, 

 propionate, salicylate, valerate, oxalate, 

 butyrate, mandelate, benzoate (0.5 per 

 cent), ethanol, methanol, n-amyl alcohol, 

 iso-amyl alcohol, tert.-butanol, n-propanol 

 and lumichrome are not utilized as carbon 

 sources. 



Starch is hydrolyzed. 



Chitin is hydrolyzed. 



Non-lipolytic. 



Nitrites not produced from nitrates. 



Ammonia produced from peptone. 



Urease-negative. 



Casein is hydrolyzed. 



Trimethylamine not produced from 

 trimethylamine oxide, choline or betaine. 



Growth inhibited by 10 per cent NaCl. 



Aerobic, facultative. 



Optimum temperature, between 20° and 

 30° C. No growth at 4° C. 



Source: Isolated from marine mud. 



Habitat: Found in sea water. 



6. Beneckea lipophaga (Campbell and 

 Williams, 1951) Campbell, comb. nov. 

 {Achromobacter lipophagiim Campbell and 

 Williams, Jour. Gen. Microbiol., 5, 1951, 

 894.) 



li.po'pha.ga. Gr. noun lipus fat; Gr. v. 

 phagein to devour; M.L. noun lipophaga fat- 

 destroyer. 



Small rods, 0.4 by 1.0 micron, occurring 

 singly, in pairs and in small clumps. Motile 

 by means of peritrichous flagella. Gram- 

 negative. 



Gelatin stab: Saccate liquefaction. 



Agar colonies: Circular, smooth, entire, 

 glistening, opaque, non-pigmented. 



Agar slant: Abundant, filiform, glisten- 

 ing, opaque, non-pigmented growth. 



Broth: Uniformly turbid. 



Litmus milk: Acid in 4 days with slight 

 peptonization. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, maltose 

 and mannose. No acid from fructose, lac- 



