FAMILY IV. ENTEROBACTERIACEAE 



357 



a'na.nas. M.L. noun Ananas generic name 

 of the pineapple. 



Note: Not to be confused with Pseudo- 

 7nonas (Phytomonas) ananas Serrano, loc. cit. 



Short rods, 0.6 bj' 0.9 micron, with 

 rounded ends, occurring singly, in pairs and 

 in short chains. Motile by means of peri- 

 trichous flagella. Encapsulated. Gram-nega- 

 tive. 



Gelatin stab: Stratiform liquefaction with 

 a deep, chrome-yellow sediment. 



Potato glucose agar: After 24 hours, circu- 

 lar, 3 mm in diameter, convex, dense, ho- 

 mogeneous, entire, moist, straw-yellow, 

 mottled, becoming primulin-yellow. Plates 

 have a molasses odor. Show two types of 

 colonies: rough and smooth. Rough colonies 

 have crenate margins. 



Potato glucose agar slant: Growth straw- 

 yellow, raised, becoming primulin-yellow, 

 moist, glistening. 



Broth: Turbid; straw-colored pellicle and 

 ring. 



Glucose broth: Growth sulfur-yellow. 



Litmus milk: Coagulated, faintly acid, be- 

 coming alkaline. 



Potato: Copious growth, moist, glisten- 

 ing, spreading, becoming primulin-yellow. 



Indole not produced. 



Slight amount of hydrogen sulfide pro- 

 duced. 



Blood serum: Moderate growth, slightly 

 raised, mustard-yellow to primulin-yellow. 

 No liquefaction after 3 months. 



Cohn's solution: No growth. 



Phenol-negative. 



Diastase-positive. 



No gas from carbohydrates. Acid from 

 glucose, lactose, sucrose, mannitol, raffi- 

 nose, glycerol, salicin, dextrin, maltose, 

 fructose and mannose. No acid from arabi- 

 nose, xylose, amygdalin, rhamnose, inositol, 

 inulin, dulcitol, adonitol, asparagine or 

 starch. 



Small amount of alcohol and aldehyde 

 produced. 



Nitrites produced from nitrates. 



Slight amount of ammonia produced. 



Source: Isolated from the pineapple {Ana- 

 nas sativus) and sugar cane (Saccharian offi- 

 cinarum). 



Habitat : Causes a brown rot of the fruit- 

 lets of pineapple. 



14. Erwinia aroideae (Townsend, 1904) 

 Holland, 1920. (Bacillus aroideae Townsend, 

 U. S. Dept. Agr., Bur. Plant Ind. Bull. 60, 

 1904, 40; Holland, Jour. Bact., 5, 1920, 222; 

 Pectobacterinm aroideae Waldee, Iowa State 

 Coll. Jour. Sci., 19, 1945, 472.) 



a.ro.i'de.ae. Gr. noun arum the plant 

 wake robin; M.L. pi. noun Aroideae the name 

 of the Arum subfamily; M.L. gen. noun 

 aroideae of an aroid. 



Description taken from Townsend (op. 

 cit., 1904, 40) and supplemented by studies 

 made by Burkholder. 



Rods, 0.5 by 2.0 to 3.0 microns; occasion- 

 ally a few very small rods occur. Motile by 

 means of 2 to 8 peritrichous flagella. Gram- 

 negative. 



Gelatin: Liquefaction. 



Beef-peptone agar slants: Light to mod- 

 erate growth, filiform, white to cream. 



Broth: Turbid; no pellicle. 



Litmus milk: Coagulation in 3 days. Lit- 

 mus reduced. One-fourth of tube whey. 



Potato plug: White with tinge of yellow. 



Endo agar slants: Streak deep red and 

 medium deep red. 



Krumwiede's triple sugar agar: Turns yel- 

 low but later a red color appears at top of 

 slant. 



Fermi's solution: Flocculent and white. 



Uschinsky's solution: Very turbid with 

 heavy sediment. 



Desoxycholate agar: Pink, later yellow- 

 ish. 



Indole not produced. 



Hydrogen sulfide produced. 



Acid but no gas from glucose, galactose, 

 fructose, arabinose, xylose, lactose, sucrose, 

 glycerol, mannitol and salicin. Maltose 

 doubtful. Alkaline reaction in sodium cit- 

 rate, and only slight growth in tartrate in 

 10 days. 



Methyl red test weakly positive; acetj'l- 

 methylcarbinol produced. 



No growth in ethanol, dulcitol, sodium 

 hippurate and malonate. 



Starch not hydrolyzed; ammonium pec- 

 tate medium liquefied. 



Nitrites rapidly produced from nitrates. 



Good growth in 5 per cent salt, and a de- 

 layed but good growth in 7 per cent salt. 



Aerobic. 



