FAMILY V. BRUCELLACEAE 



403 



agglutination with Bordetella parapertussis 

 and B. bronchiseptica. B. pertussis antitoxin 

 neutralizes toxin of B. parapertussis and of 

 B. bronchiseptica. 



Source: Isolated from the respiratory 

 tract in cases of whooping cough. 



Habitat: Etiologically associated with 

 whooping cough. 



2. Bordetella parapertussis (Eldering 

 and Kendrick, 1937) Moreno-L6pez, 1952. 

 (Eldering and Kendrick, Jour. Bact., 33, 

 1937, 71; Bacillus para-pertussis Eldering 

 and Kendrick, Jour. Bact., 35, 1938, 561; 

 Haemophilus parapertussis Topley and 

 Wilson, Princip. Bact. and Immun., 3rd 

 ed., ), 1946, 802; Moreno-Lopez, Microbiol. 

 Espanola, 5, 1952, 177.) 



pa.ra.per.tus'sis. Gr. prep, para along- 

 side of, resembling; M.L. noun pertussis a 

 specific epithet; M.L. adj. parapertussis 

 (Bordetella) pertussis-like. 



Morphologically similar to Bordetella 

 pertussis. Non-motile. Gram-negative. 



Bordet-Gengou agar colonies: Like those 

 of B. pertussis; surrounding medium is 

 darkened followed by hemolysis. Growth in 

 1 to 2 days. 



Peptone agar colonies: Like those on Bor- 

 det-Gengou agar. Brown coloring of medium. 



Broth: Ropy sediment; brown coloring 

 of medium. 



Litmus milk: Alkaline in 2 to 4 days. 



Indole not produced. 



Carbohydrates not attacked. 



Citrate utilized as a sole source of carbon. 



Nitrites not produced from nitrates. 



Urea usually split. 



Catalase-positive. 



Aerobic. 



Optimum temperature, about 37° C. 



Serologically homogeneous. Shows cross 

 agglutination with Bordetella pertussis 

 and B. bronchiseptica. Toxin neutralized by 

 antitoxin of jB. pertussis and of B. bronchi- 

 septica. 



Source: Isolated from the respiratory 

 tract in cases of a whooping-cough-like 

 disease. 



Habitat: Etiologically associated with a 

 whooping-cough-like disease. 



3. Bordetella bronchiseptica (Ferry, 

 1912) Moreno-Lopez, 1952. (Short, narrow 



bacillus, Ferry, Amer. Vet. Rev., 37, 1910, 

 499; also see McGowan, Jour. Path, and 

 Bact., 15, 1911, 372; Bacillus bronchicanis 

 Ferry, Jour. Inf. Dis., 8, 1911, 402; Bacillus 

 bronchisepticus Ferry, Amer. Vet. Rev., 

 41, 1912, 79; Bacterium bronchisepticus 

 Evans, Jour. Inf. Dis., 22, 1918, 580; Alcali- 

 genes bronchisepticus Bergey et al., Manual, 

 1st ed., 1923, 234; Brucella bronchiseptica 

 Topley and Wilson, Princip. Bact. and 

 Immun., 1st ed., 1, 1931, 508; Haemophilus 

 bronchisepticus Topley and Wilson, ibid., 

 3rd ed., 1, 1946, 787; Moreno-Lopez, Micro- 

 biol. Espanola, 5, 1952, 177.) 



bron.chi.sep'ti.ca. Gr. noun bronchus 

 the trachea; Gr. adj. septicus putrefactive, 

 septic; M.L. adj. bronchisepticus apparently 

 intended to mean "with an infected bron- 

 chus." 



Morphologically similar to Bordetella 

 pertussis. Motile by means of peritrichous 

 fiagella. Gram-negative. 



Gelatin colonies: Similar to those on agar. 

 No liquefaction. 



Nutrient agar colonies: Smooth, raised, 

 entire, pearly, glistening. No brown dis- 

 coloration of medium. 



Blood agar colonies: Similar to those on 

 agar; surrounded by a zone of hemolysis. 

 Rapid growth. 



Broth: Turbid; thin, gray pellicle; ropy 

 sediment. 



Litmus milk: Alkaline in 1 to 2 days. 



Indole not produced. 



Carbohydrates not attacked. 



Citrate utilized as a sole source of carbon. 



Nitrites often produced from nitrates 

 (Topley and Wilson). 



Urea and asparagin are split. 



Catalase-positive. 



Aerobic. 



Optimum temperature, 37° C. 



Shows cross agglutination with Bordetella 

 pertussis and B. parapertussis. Toxin 

 indistinguishable from that of B. pertussis 

 and of B. parapertussis. 



Source: Isolated from dogs affected with 

 distemper. 



Habitat: Etiologically associated with 

 bronchopneumonia in rodents, broncho- 

 pneumonia-complicating distemper in dogs 

 and occasionally with a whooping-cough- 

 like disease in man. 



