FAMILY V. BRUCELLACEAE 



411 



Microaerophilic, facultatively anaerobic. 

 Grows best in the presence of CO2 . 



Optimum temperature, 37° C. Killed in 

 10 minutes at 60° C. 



Non-pathogenic for guinea pigs and mice. 



Source : Isolated from the blood and heart 

 valve of a case of endocarditis. 



Habitat: Found in the blood and on the 

 heart valve of one case of endocarditis. 



9. Haemophilus influenzae -murium 



(Kairies and Schwartzer, 1936) Lwoff, 1939. 

 {Bacterium influenzae murium (sic) Kairies 

 and Schwartzer, Cent. f. Bakt., I Abt., 

 Orig., 1S7, 1936, 351; Haemophilus influenzae 

 murium (sic) Lwoff, Ann. Inst. Past., 62, 

 1939, 168.) 



in.flu.en'zae-mu'ri.um. Italian noun in- 

 fluenza influenza; L. noun mus, muris 

 mouse; M.L. gen. noun influenzae -murium 

 influenza of mice. 



Small, short, thick coccobacilli occurring 

 singly and at times in the form of threads. 

 Filterable forms pass through 0.4- to 0.6- 

 micron pores. Non-motile. Shows bipolar 

 staining. Gram-negative. 



Requires the factor X for growth (Ivano- 

 vics and Ivanovics, Cent. f. Bakt., I Abt., 

 Orig., 139, 1937, 184). 



Gelatin: No liquefaction. 



Levinthal agar colonies: Up to 4 mm in 

 diameter, round, convex, glistening, bluish, 

 transparent. Resemble Haemophilus in- 

 fluenzae colonies. Become opaque and whit- 

 ish with age. 



Blood agar colonies. Whitish. No hemoly- 

 sis. 



Chocolate agar: Good growth. 



Endo agar: Red colonies in 2 to 3 days. 



Levinthal or egg broth: Uniformly 

 turbid. 



Milk not coagulated. 



Indole not produced. 



Acid from glucose, fructose, lactose, 

 maltose and sucrose. No acid from man- 

 nite. 



Aerobic. 



Optimum temperature, 37° C. 



Slightly pathogenic for mice but not 

 for guinea pigs. 



Serologically homogeneous and distinct 

 from Haemophilus influenzae, Pasteurella 

 and Salmonella typhosa. 



Source: Isolated from the noses and 

 pharynges of mice. 



Habitat: Causes conjunctivitis and re- 

 spiratory infections in mice. 



10. Haemophilus ovis Mitchell, 1925. 

 (Jour. Amer. Vet. Assoc, 68, 1925, 8.) 



o'vis. L. fem.n. ovis a sheep; L. gen. noun 

 ovis of a sheep. 



Small, short, somewhat pleomorphic 

 rods occurring singly and at times in short 

 chains. Coccoid forms occur in old cultures. 

 Non-motile. Gram-negative. 



Requires the X factor when newly iso- 

 lated. After serial passage on chocolate 

 agar, slight growth on plain agar medium. 

 Primary isolation best on chocholate blood 

 agar. 



Gelatin slant: Very slight growth in 4 

 days. 



Gelatin stab: No growth. 



Chocolate blood agar colonies: After 24 

 to 36 hours, pinhead in size, discrete, moist, 

 viscid, translucent; become gray, scaly 

 and adherent to the medium with age. 



Blood broth: No hemolysis. 



Plain broth: After adaptation, turbid. 

 Floating ropy strands. Slimy sediment. 



Litmus milk: No change. 



Potato: No growth. 



Indole not produced. 



Acid but no gas from glucose, fructose, 

 galactose, sucrose, raffinose, sorbitol, man- 

 nitol, mannose and maltose. Weak acidity 

 from lactose and xylose. No acid from 

 arabinose, rhammose, salicin or inositol. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 37° C. Slight 

 growth at 28° C. 



Pathogenic for guinea pigs and sheep. 



Source: Isolated from the lungs of sheep. 



Habitat: Causes bronchial pneumonia 

 and generalized hemorrhagic involvement 

 in sheep. 



11. Haemophilus putoriorum Haudu- 

 roy et al., 1937. {Bacterium influenzae 

 putoriorum multiforme Kairies, Ztschr. f. 

 Hyg., 117, 1935, 12; Hauduroy et al.. Diet, 

 d. Bact. Path., 1937, 258.) 



pu.to.ri.o'rum. M.L. mas.n. Putorius 



