420 



ORDER IV. EUBACTERIALES 



Neumann, Bakt. Diag., 2 Aufl., 2, 1899 

 (July), 193; Bacterium conjuncHvitidis 

 Chester, Man. Determ. Bact., 1901, 120; 

 not Bacterium conjunctivitidis Migula, 

 Syst. d. Bact., 2, 1900, 509; Diplobacillvs 

 moraxaxenfeld McNab, Klinische Monatsbl. 

 f. Augenheilk., J^2, 1904, 64; Bacillus duplex 

 Hewlett, Med. Res. Council Syst. of Bact., 

 2, 1929, 417; Lwoff, Ann. Inst. Past., 62, 

 1939, 173.) 



la.cu.na'ta. L. noun lacuna a hollow or 

 cavity; M.L. adj. lacunatus pitted. 



Short rods, 0.4 to 0.5 by 2.0 microns, 

 occurring singly, in pairs and in short 

 chains, with ends rounded or square in the 

 chains. Non-motile. Gram-negative. 



Requires the addition of blood serum for 

 growth in bouillon or peptone water. 



Gelatin colonies: No growth. 



Gelatin stab: No growth. 



Blood agar colonies: No growth on pri- 

 mar}^ isolation. Growth on subculture is 

 difficult. Certain strains are not surrounded 

 by zones of hemolysis, while others are 

 (Oag, Jour. Path, and Bact., 54, 1942, 128). 



Serum agar colonies: No growth on pri- 

 mary isolation. 



Loffler's blood serum: Slow but definite 

 liquefaction with deep pitting around the 

 colonies. 



Ascitic broth: Turbid with slight, grayish 

 sediment. 



Blood milk mixture: Doubtful develop- 

 ment. 



Litmus milk: No growth. 



Potato: No growth. 



Various carbohydrates and mannitol are 

 attacked. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 37° C. 



Comments: Audureau (Ann. Inst. Past., 

 64, 1940, 128) reports a variety of this organ- 

 ism which differs from the parent strain in 

 that it does not liquefy serum. 



Source: Isolated from conjunctiva. 



Habitat: Causes subacute infectious con- 

 junctivitis, or angular conjunctivitis. 



2. Moraxella liquefaciens (McNab, 

 1904) Murray, 1948. (Diplobacille liqu^fiant, 

 Pettit, Annales d'oculistique, March, 1899, 

 166; also see Thesis, Paris, 1900, 223; Diplo- 

 hacilbis liquefaciens McNab, Klinische 



Monatsbl. f. Augenheilk., 42, 1904, 64; 

 Murray, in Manual, 6th ed., 1948, 591.) 



li.que.fa'ci.ens. L. part. adj. liquefaciens 

 dissolving. 



Rods, 1.0 to 1.5 by 2.0 microns, with 

 rounded ends, occurring in pairs and some- 

 times singly. Capsules not demonstrated. 

 Non-motile. Stain uniformly with basic 

 aniline dyes. Gram-negative. 



Will barely grow in bouillon or peptone 

 water without the addition of blood serum. 



Gelatin colonies: Round, 1.5 to 2.0 mm in 

 diameter, j-ellowish white. 



Gelatin stab: Rapid liquefaction. 



Blood agar: Ready growth in primarj- and 

 subculture. 



Ascitic agar colonies: Grayish, thick, 

 round, viscous. 



Peptone agar colonies: Same as above, 

 but less abundant growth. 



Coagulated serum: Liquefaction in 3 to 

 4 days; eventually complete. 



Plain broth: Poor growth, if any. Slight, 

 uniform turbidity. 



Ascitic broth: Abundant growth in 24 

 hours at 35° C. Uniform turbidity. Later 

 sediment and an opaque pellicle. 



Milk: No growth. No coagulation. 



Potato: Slight, yellowish white, viscous 

 growth. 



Optimum temperature, between 20° and 

 37° C. Killed at 55° C for 15 minutes. 



Aerobic. 



Not pathogenic for laboratory animals. 



Source: Isolated from cases of conjuncti- 

 vitis associated with corneal ulceration in 

 man. 



Habitat: Conjunctivitis in man so far as 

 known. 



3. Moraxella bovis (Hauduroy et al., 

 1937) Murray, 1948. (Diplobacillus, Allen, 

 Jour. Amer. Vet. Med. Assn., 54, 1918, 307; 

 Diplobacillus, Jones and Little, Jour. Exp. 

 Med., 38, 1923, 139; Hemophilus bovis Hau- 

 duroy et al.. Diet. d. Bact. Path., 1937, 247; 

 Murray, in Manual, 6th ed., 1948, 591.) 



bo'vis. L. noun bos cow, ox; L. gen. noun 

 bovis of an ox or cow. 



Short, plump rods, 0.5 by 1.5 to 2.0 mi- 

 crons, with rounded ends, usually occurring 

 in pairs and in short chains. Encapsulated. 

 Non-motile. Gram-negative. 



