FAMILY V. BRUCELLACEAE 



421 



Gelatin: Slow growth at 22° C. Ver}^ slow 

 liquefaction. 



Blood agar colonies: After 24 hours, 

 round, translucent, grayish white, sur- 

 rounded by a narrow, clear zone of hemoly- 

 sis ; deep colonies tiny with a clear hemolytic 

 zone, usually 1.5 mm in diameter. After 48 

 hours, surface colonies somewhat flattened, 

 3.5 to 4.0 mm in diameter; deep colonies 

 ellipsoidal and biconvex with hemolytic 

 area 2.5 to 3.0 mm in diameter. 



Blood agar slants : After 24 hours at 38° C. , 

 heavy, viscid, grayish white growth. 



Coagulated serum: Liquefaction. 



Broth: Slow growth; slight turbidity; 

 considerable sediment. 



Litmus milk: Alkaline. Partial coagula- 

 tion. 



Potato: No growth. 



Indole not produced. 



No acid from glucose or other carbohy- 

 drates. 



Nitrites not produced from nitrates. 



Aerobic. 



Optimum temperature, 36° C. Killed at 

 58° to 59° C. in five minutes. 



Not pathogenic for laboratory animals. 



Source: Isolated from acute cases of 

 ophthalmia (pink eye) of cattle. 



Genus VIII. Noguchia Olilsky et al., 1934.* 

 (Olitsky, Syverton and Tyler, Jour. Exp. Med., 60, 1934, 382.) 



No.gu'chi.a. M.L. noun Noguchia named for Hideyo Noguchi, the bacteriologist who 

 isolated the type species of this genus. 



Small, slender rods. Encapsulated. Motile by means of peritrichous flagella. Gram-nega- 

 tive. Produce a mucoid type of growth which, on initial isolation, occurs with some diffi- 

 culty on ordinary media. Aerobic to facultatively anaerobic. Optimum temperature, be- 

 tween 28° and 30° C. Found in the conjunctiva of man and animals affected by a follicular 

 type of disease. 



The type species is Noguchia granulosis (Noguchi) Olitsky et al. 



Ketj to the species of genus Noguchia. 



I. Produce acid from carbohydrates. 



A. Produces acid from raffinose, maltose and salicin. 



1. Noguchia granulosis. 



B. Does not produce acid from raffinose, maltose or salicin. 



2. Noguchia simiae. 

 II. Does not produce acid from carbohydrates. 



3. Noguchia cxmiculi. 



1. Noguchia granulosis (Noguchi, 1928) 

 Olitsky et al., 1934. {Bacterium granulosis 

 Noguchi, Jour. Exp. Med., 48, 1928, Supple- 

 ment 2, 21; Olitsky, Syverton and Tyler, 

 Jour. Exp. Med., 60, 1934, 382.) 



gra.nu.lo'sis. L. noun granulum a small 

 grain; Gr. suffix -osis; M.L. fem.n. granulosis 

 pathological condition characterized by col- 

 lection of granules; M.L. gen. noun granulo- 

 sis of granulosis. 



Rods measuring 0.25 to 0.3 by 0.8 to 1.2 

 microns in young cultures; involution forms 

 occur in old cultures on blood agar. Motile 

 by means of a single flagellum which usually 



arises from one of the poles but which occa- 

 sionally appears as if attached to one side. 

 Gram-negative. 



No growth on plain agar or broth. 



Blood agar colonies: Minute, round, 

 shiny, somewhat raised, almost transparent 

 or slightly grayish in 48 hours; later the 

 colonies increase in size and are grayish, 

 opalescent and somewhat sticky. Old col- 

 onies have a brownish or yellowish tint. 



Semi-solid Leptospira medium: Grayish 

 white, diffuse growth forming a delicate 

 zone 1 cm deep. 



Liquid Leptospira medium: Diffuse, 



Arranged by Prof. C. D. Kelly, McGill University, Montreal, P.Q., Canada, October, 



1938. 



