422 



OEDER IV. EUBACTERIALES 



slightly cloudy growth; sticky, grayish 

 sediment at the bottom of the tube in old 

 cultures. 



Acid from glucose, fructose, mannose, 

 sucrose, galactose, maltose, salicin, xylose, 

 mannitol, dextrin, arabinose, amygdalin 

 and lactose. Small amount of acid from 

 raffinose, inulin, rhamnose and trehalose. 

 No acid from dulcitol, sorbitol or inositol. 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, be- 

 tween 15° and 30° C. Grows at 37° C. 



Optimum pH, 7.8. 



Non-pathogenic for rabbits, guinea pigs, 

 rats and mice. 



Distinctive characters: Non-motile when 

 cultured on Leptospira medium or on blood 

 agar plates at 30° to 37° C; motile when 

 grown on horse blood agar slants at 30° C. 

 or on Leptospira medium or blood agar 

 plates at 15° C. Carbohydrates are attacked. 

 Agglutination reactions. 



Source : Isolated from cases of trachoma of 

 American Indians at Albuquerque, New 

 Mexico. 



Habitat: Regarded by Noguchi and by 

 others as a cause of trachoma in man. Pro- 

 duces a granular conjunctivitis in monkej^s 

 and apes. 



2. Noguchiasimiae (Olitsky et al., 1933) 

 Olitsky et al., 1934. (Bacterium simiae 

 Olitsky, Syverton and Tyler, Jour. Exp. 

 Med., 57, 1933, 875; Olitsky, Syverton and 

 Tyler, ibid., 60, 1934, 382.) 



si'mi.ae. L. noun simia ape; L. gen. noun 

 simiae of an ape. 



Slender rods, 0.2 to 0.3 by 0.8 to 1.2 mi- 

 crons, with pointed ends, occurring singly, 

 in pairs, in short chains or in a parallel 

 arrangement of two or three cells. Encapsu- 

 lated. Motile by means of flagella which 

 are really of the peritrichous type, although 

 normally not more than one flagellum is 

 found attached near the end but usually not 

 in the polar position; occasionally two 

 separated flagella are observed in lateral 

 positions. Gram-negative. 



Gelatin colonies: More mucoid and more 

 raised than those on agar. 



Gelatin stab: Arachnoid growth along line 

 of inoculation. No liquefaction. 



Agar colonies: Small, circular, grajdsh, 

 translucent, smooth, convex, slightly raised 

 with a sticky or mucoid consistency. 



Agar slants: Grayish white to white, 

 moist, mucoid, raised, glistening growth. 

 Growth is more profuse when blood is added. 



Blood agar colonies : More highly translu- 

 cent and colorless during earlj- growth than 

 those on plain agar, becoming grayish after 

 two or three daj's. 



Leptospira medium: Homogeneous, dense 

 growth in a 0.5 cm, sharply defined layer 

 with a slight, nebulous, uniform opacity 

 about 1 cm below. In three or four days the 

 lower layer becomes more dense and in time 

 extends to the bottom of the tube. 



Broth: Uniformly turbid; slight, grayish 

 sediment; no pellicle. 



Litmus milk: Unchanged. 



Potato: Light tan, spreading, abundant 

 growth. 



Indole not produced. 



Acid but no gas from glucose, fructose, 

 mannose, galactose, xylose, arabinose and 

 rhamnose. Small amount of acid from dex- 

 trin. Some strains produce a small amount 

 of acid from sucrose, lactose, inulin and 

 mannitol. Raffinose, salicin, dulcitol, amyg- 

 dalin, maltose, trehalose, sorbitol and inosi- 

 tol are not attacked. 



Nitrites not produced from nitrates. 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, be- 

 tween 28° and 30° C. Thermal death point, 

 56° C. for thirty minutes. 



Serological reactions: Rabbit antiserum 

 is specific for all strains, and there is no 

 cross agglutination with Noguchia granulo- 

 sis. 



Distinctive characters: Attacks carbohy- 

 drates. Agglutination reactions. 



Source: Isolated from inflammatory type, 

 Type II, of spontaneous conjunctival 

 folliculosis in monkeys (Macacus rhesus). 



Habitat: Causes conjunctival folliculosis 

 in monkeys (Macacus rhesus). 



3. Noguchia cuniculi Olitsky et al., 

 1934. (Olitsky, Syverton and Tyler, Jour. 

 Exp. Med., 60, 1934, 382.) 



cu.ni'cu.li. L. noun cunioilns rabbit; L. 

 gen. noun cuniculi of a rabbit. 



