440 



ORDER IV. EUBACTERIALES 



Diet. d. Bact. Path., 1953, 259). Survives 

 56° C. for 15 minutes but not 60° C. for 10 

 minutes (Hine and Berry). 



Source: One strain was isolated from a 

 deposit on teeth in a healthy mouth 

 (Knorr) ; also isolated from gingival crev- 

 ices in normal mouths, from cases with 

 deep caries, inflamed areas around crowns 

 and fillings, pyorrhetic pockets, Vincent's 

 infection and from the mouths of three 

 monkeys and two rabbits (Hine and Berry) ; 

 also found in an actinomycosis of the face 

 (Robin). 



Habitat: Presumably the buccal cavity. 



5. Fusobacterium vescum (Eggerth 

 and Gagnon, 1933) Hoffman, comb. nov. 

 (Bacteroides vescus Eggerth and Gagnon, 

 Jour. Bact., 25, 1933, 406; Fusijormis vescus 

 Prevot, Ann. Inst. Past., 60, 1938, 300.) 



ves'cus. L. adj. vescus small, weak. 



Slender, pointed rods, 1 to 2 microns long, 

 which are sometimes slightly curved. Non- 

 motile. Show bipolar staining. Gram-nega- 

 tive. 



Gelatin: Liquefaction in 8 to 25 days. 



Blood agar colonies: Very minute and 

 transparent. 



Broth: Diffusely clouded. 



Milk: Neither acidified nor coagulated. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from cellobiose (in 30 

 days), dextrin, glucose, maltose, mannose 

 and rhamnose. No acid or gas from esculin, 

 amygdalin, arabinose, galactose, mannitol, 

 melezitose, raffinose, salicin, sorbitol, 

 starch, sucrose, trehalose, xylose, glycerol, 

 glycogen, inulin, lactose or fructose. 



Nitrites not produced from nitrates. 



Peptone: No gas. 



Anaerobic. 



Non-pathogenic for white mice or rabbits. 



Source: One strain was isolated from 

 human feces. 



Habitat: Probably the intestinal canals 

 of mammals. 



6. Fusobacterium biacutum Weinberg 

 and Prevot, 1926. (Compt. rend. Soc. Biol., 

 Paris, 95, 1926, 519.) 



bi.a.cu'tum. L. adv. bis twice; L. adj. 

 acutus sharp, pointed; L. part. adj. biacutus 

 two-pointed. 



Rods, 0.4 to 0.5 by 1.4 to 3.0 microns, with 

 pointed ends, occurring singly, in pairs or 

 sometimes in short chains. Non-motile. 

 Gram-negative. 



Gelatin: No liquefaction. 



Veillon's agar: Rapid growth. Colonies 

 lens-shaped. Gas is produced which breaks 

 up the medium. 



Plain broth: Poor growth. 



Glucose broth: Turbid. Medium rapidly 

 acidified. Good growth. 



Indole not produced. 



Small amount of hydrogen sulfide pro- 

 duced. 



Milk: Acid and coagulation in 2 to 8 days. 

 Curd not digested. 



Casein and coagulated egg-white not 

 digested. 



Acid from glucose, fructose, galactose, 

 maltose and lactose. 



Neutral red reduced. 



Does not require blood serum for growth. 



Anaerobic. 



Killed in 60 minutes at 60° C. 



Pathogenic for guinea pigs. 



Source: Six strains were isolated from a 

 case of appendicitis. 



Habitat: Unknown. 



Genus III. Dialister Bercjey et al., 1923* 

 (Manual, 1st ed., 1923, 271.) 



Di.a.lis'ter. Etymology uncertain. 



Minute, filterable, rod-shaped cells, with rather pointed ends, occurring singly, in pairs 

 and in short chains. Non-motile. Gram-negative. Require fresh, sterile tissue or ascitic 

 fluid for growth. Glucose is fermented. Anaerobic. Parasitic. Found, associated with in- 

 fluenza, in the respiratory tract. 



The type species is Dialister p7ieuinosintes (Olitsky and Gates) Bergey et al. 



* Arranged by Dr. A. Parker Kitchens, University of Pennsylvania, Philadelphia, Penna., 

 March, 1946. 



