FAMILY VI. BACTEROIDACEAE 



447 



Source: Seven strains were isolated from 

 human feces. 



Habitat: Probably the intestinal canals 

 of mammals. 



9. Sphaerophorus niortiferus (Harris, 

 1901) Prevot, 1938. {Bacillus mortiferus 

 Harris, Jour. Exp. Med., 6, 1901, 519; 

 Prevot, Ann. Inst. Past., 60, 1938, 299.) 



mor.ti'fer.us. L. noun mors death; L. 

 gen. noun mortis of death; L. v. fero to bear; 

 ALL. adj. mortiferus death-bearing. 



Rods, 0.6 bj' 2.0 microns, with rounded 

 ends, occurring singly, in pairs or in short 

 chains, with filamentous and pleomorphic 

 forms, some showing metachromatic gran- 

 ules. Non-motile. Gram-negative. 



Serum or ascitic fluid is required for 

 growth. 



Hydrocele gelatin: No liquefaction. No 

 gas. 



Hydrocele agar: Deep colonies small, ir- 

 regular, translucent, granular, light brown. 

 Surface colonies small, regular or fringed, 1 

 to 2 mm in diameter, yellowish brown; fecal 

 odor. 



Glucose-human blood agar: Gas with a 

 disagreeable odor; browning of the hemo- 

 globin. 



Hydrocele broth: Growth with heavy, 

 viscous, whitish deposit and foul-smelling 

 gas. 



Hj^drocele milk: Acid but no coagulation. 

 In 6 days the milk is peptonized. 



Indole produced in hydrocele broth. 



Hydrogen sulfide produced in hydrocele 

 broth. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for guinea pigs, rabbits and 

 mice. 



Source: Isolated from a liver abscess of 

 man. 



Habitat : Presumabl}^ found in various in- 

 fections in man. 



10. Sphaerophorus gonidiaformans 



(Tunnicliff and Jackson, 1925) Prevot, 1938. 

 (Bacillus gonidiaformans Tunnicliff and 

 Jackson, Jour. Inf. Dis., 36, 1925, 430; 

 Actinomyces gonadiformis (sic) Bergey et al., 

 Manual, 3rd ed., 1930, 469; Bacteroides goni- 

 diaformans Hauduroy et al.. Diet. d. Bact. 



Path., 1937, 62; Prevot, Ann. Inst. Past., 

 60, 1938, 299.) 



go.ni.di.a.for'mans. Gr. noun gone off- 

 spring, seed; M.L. noun gonidium gonidium; 

 L. part. adj. formans forming; M.L. adj. 

 gonidiaformans gonidia-forming. 



Rods, 0.5 by 1.0 to 3.0 microns, with 

 rounded ends; gonidia form within the 

 rods, developing into short or long, wavy 

 filaments. Non-motile. Show bipolar stain- 

 ing; also show red granules with Giemsa 

 stain. Gram-negative. 



Serum or ascitic fluid is required for 

 growth. 



Gelatin: No liquefaction. No growth. 



Blood agar colonies: Thick,whitish, moist, 

 entire. 



Glucose media: Slight gas produced. 



Broth: Slight development; flocculent 

 sediment. 



Litmus milk: No growth. 



Potato: No growth. 



No acid or gas from glucose, salicin, man- 

 nitol or inulin. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for rabbits and guinea pigs. 



Source : Isolated from tonsils. 



Habitat: Presumably the pharynx. 



11. Sphaerophorus freundii (Haudu- 

 roy et al., 1937) Prevot, 1938. (Freund, Cent, 

 f. Bakt., I Abt., Ong., 88, 1922, 9; Bacterium 

 of Freund, Weinberg et al., Les Microbes 

 Anaerobies, 1937, 706; Bacteroides freundii 

 Hauduroy et al., Diet. d. Bact. Path., 1937, 

 bl ; Sphaerophorus freundi (sic) Prevot, Ann. 

 Inst. Past., 60, 1938, 299.) 



freun'di.i. M.L. gen. noun freundii of 

 Freund; named for F. Freund, the bacteri- 

 ologist who first described this species. 



Short, ellipsoidal rods, 0.3 to 0..35 by 0.8 

 to 1.0 micron, occurring singly, in pairs and 

 in chains. Pleomorphic in cultures with ir- 

 regular filaments and swollen forms. Non- 

 motile. Show bipolar staining. Gram- 

 negative. 



Serum or ascitic fluid is required for 

 growth. 



Gelatin: No liquefaction. 



Ascitic agar: Deep colonies lenticular in 1 

 to 3 days, small, yellowish. 



