448 



ORDER IV. EUBACTERIALES 



Serum glucose agar: Deep colonies len- 

 ticular. Gas produced. 



Glucose agar: Surface colonies pinhead, 

 dew-drop, opaque, yellowish. 



Glucose serum broth: Turbidity; thin 

 fiocculent growth. Gas produced with fetid 

 odor. 



Serum milk: Coagulated in 3 daj-s. Not 

 peptonized. 



Indole is produced in glucose broth. 



Hydrogen sulfide is produced in glucose 

 broth. 



Coagulated hydrocele fluid not liquefied. 



Acid and gas from glucose, maltose, su- 

 crose and inannitol. I']thanol and butyric 

 and lactic acids are produced from carbohy- 

 drates. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for rabbits and guinea pigs. 



Source: One strain was isolated from a 

 purulent meningitis following otitis in man. 



Habitat: Found in various infections in 

 man. 



12. Sphaerophorus pyogenes (Haudu- 

 roy et al., 1937) Prevot, 1938. (Buday, Cent, 

 f. Bakt., I Abt., Orig., 77, 1916, 453; Bacillus 

 pyogenes anaerobins Bela-Johan, Cent. f. 

 Bakt., I Abt., Orig., 87, 1922, 290; not 

 Bacillus pyogenes anaerobius Kruse, in 

 Fliigge, Die Mikroorganismen, 3 Aufl., 2, 

 1896, 244; Bacteroides pyogenes Hauduroy 

 et al.. Diet. d. Bact. Path., 1937, 69; Prevot, 

 Ann. Inst. Past., 60, 1938, 299.) 



py.o'ge.nes. Gr. noun pyum pus; Gr. v. 

 gennaio to produce; M.L. adj. pyogenes pus- 

 producing. 



Short, ellipsoidal rods, occurring singly, 

 in short chains and as pleomorphic, filamen- 

 tous forms. Non-motile. Show bipolar stain- 

 ing. Gram-negative. 



Serum or ascitic fluid is required for 

 growth; isolation in culture media is easier 

 after animal passage. 



Serum agar: Deep colonies small, puncti- 

 form. Some gas produced. Surface colonies 

 fine, transparent, with flat borders, 0.5 mm 

 in diameter. 



Ascitic broth: The optimum concentra- 

 tion of ascitic fluid is from 30 to 50 per cent. 

 Fiocculent growth with a foamy layer on the 

 surface. Gas produced. 



Tarozzi broth with liver: Rapid growth 

 with turbidity and later a fiocculent deposit. 

 Foamy layer on the surface. 



Hydrogen sulfide not produced. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenicity: Pathogenic for rabbits 

 and guinea pigs when pus from a lesion is in- 

 jected. Cultures other than those in ascitic 

 broth show no pathogenicity. 



Source: Isolated from abscesses of the 

 liver and lungs following septic war wounds; 

 also isolated from the blood stream follow- 

 ing tonsillectomies. 



Habitat: Found in various infections in 

 man. 



13. Sphaerophorus influenzaeforniis 



(Russ, 1905) Prevot, 1938. (Bacillus influen- 

 zaeformis Russ, Cent. f. Bakt., I Abt., 

 Orig., 39, 1905, 357; Bacteroides russii Hau- 

 duroy et al.. Diet. d. Bact. Path., 1937, 73; 

 Prevot, Ann. Inst. Past., 60, 1938, 299.) 



in.flu.en.zae.for'mis. M.L. noun iyiflu- 

 enza influenza; L. noun forma shape; M.L. 

 adj. influenzaeformis influenza-like. 



Short rods, 0.5 by 1.5 microns, with 

 rounded ends ; some strains are pleomorphic 

 with involution forms and swollen fila- 

 ments. Non-motile. Show bipolar staining. 

 Gram-negative. 



Gelatin: No liquefaction. 



Agar: Deep colonies lenticular, sometimes 

 surrounded by a number of smaller colonies. 

 No gas. 



Glucose agar: Surface colonies semi- 

 transparent, dew-drop, grayish. 



Peptone broth: No growth. 



Glucose broth: Uniform turbidity which 

 deposits as a granular mass. No gas pro- 

 duced. 



Milk: Slow growth with coagulation in 

 several weeks. No gas or digestion of coagu- 

 lum. 



Indole produced in glucose broth. 



Hydrogen sulfide produced in glucose 

 broth. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenicity: One strain was patho- 

 genic for rabbits, mice and guinea pigs. 



Source: One strain was isolated from a 



