450 



ORDER IV. EUBACTERIALES 



Serum gelatin: No liquefaction. 



Serum agar: Surface colonies small, trans- 

 lucent, slightly raised, adherent to medium 

 in 48 hours. Deep colonies lenticular, 2 mm 

 in size in 48 hours. Colonies difficult to 

 break up. No gas. 



Serum broth: Supernatant fluid clear with 

 small, stellate colonies which tend to adhere 

 to walls of the tube. No gas. 



Milk: Unchanged. 



Coagulated egg white and serum not 

 liquefied. 



Indole not produced in serum peptone 

 water. 



Hydrogen sulfide not produced. 



No acid or gas from carbohydrates. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for guinea pigs, rabbits and 

 mice. 



Source: Isolated from epidemic benign 

 cervical adenitis of guinea pigs. 



Habitat: Infected guinea pigs so far as 

 known. 



17. Sphaerophorus glycolyticus Tar- 

 dieux and Ernst, 1951. (Tardieu.x and Ernst, 

 in Tardieux, Ann. Inst. Past., 80, 1951, 278.) 



gly.co.ly'ti.cus. Gr. adj. glycus sweet; 

 Gr. adj. lyticus dissolving; M.L. adj. glyco- 

 lyticus sugar-dissolving. 



Pleomorphic rods which, on initial cul- 

 turing, occur as ellipsoidal forms, 2 to 3 

 microns in diameter, and as elongated forms 

 with lateral or terminal, spheroidal swell- 

 ings and metachromatic granules. With 

 subsequent transfers, the short forms pre- 

 dominate, especially in liquid media, and 

 pleomorphism is retained in agar stabs. The 

 ellipsoidal forms show bipolar staining. 

 Non-motile. Gram-negative. 



Serum or ascitic fluid is required for 

 growth. 



No gas, but abundant odor is produced. 



Gelatin: Slow liquefaction (sometimes 

 only after 1 month). 



Agar stab: Colonies are punctiform or 

 lenticular. 



Glucose broth: Abundant, homogeneous 

 turbidity. 



In glucose broth, the end-products of 

 fermentation are acetic, butyric and lactic 

 acids, hydrogen sulfide, ammonia, acetone 



and traces of aldehydes and skatole. Amines 

 and alcohols are not produced. 



Peptone serum broth: Very slight tur- 

 bidity. 



Milk: Rapid coagulation and digestion. 



Coagulated proteins not attacked. 



Acid but no gas from glucose, fructose, 

 galactose, sucrose, lactose, maltose and 

 glycerol. 



Starch is not hydrolyzed. 



Nitrites not produced from nitrates. 



Neutral red is reduced, progressively re- 

 coloring. 



Anaerobic. 



Survives for several minutes at 70° C. 



Neither to.xin nor hemolysin produced. 



Pathogenic for guinea pigs but not for 

 rabbits. 



Source: Two strains were isolated from 

 genital infections and a third from a war 

 wound. 



Habitat: Human sources so far as known. 



18. Sphaerophorus bullosus (Distaso, 

 1912) Breed, comb. nov. (Bacillus bullosus 

 Distaso, Cent. f. Bakt., I Abt., Orig., 62, 

 1912, 443; Bacteroides bullosus Castellani 

 and Chalmers, Man. Trop. Med., 3rd ed., 

 1919, 960; Spherocillus bullosis (sic) Pr^vot, 

 Ann. Inst. Past., 60, 1938, 300 (type species 

 of genus Sphaerocillus Prevot, ibid., 297).) 



bul.lo'sus. L. noun6MZZaaknob;M.L. adj. 

 bullosus knobbed. 



Small rods with rounded ends; at times 

 long, slender, bifurcating forms occur with 

 an enlargement at one pole or in the center. 

 Motile by means of peritrichous flagella. 

 Show bipolar staining. Gram-negative. 



Gelatin: No liquefaction. 



Glucose agar colonies: Deep colonies very 

 small with fimbriate margin. Gas is pro- 

 duced. 



Glucose broth: Flocculent growth falling 

 to bottom. Some gas produced. 



Litmus milk: Slight acidity. No coagula- 

 tion. 



Indole not produced. 



Acid from glucose. No acid from lactose or 

 sucrose. 



Anaerobic. 



Optimum temperature, 37° C. 



Source: Isolated from the intestinal tract. 



Habitat: Found in the intestinal tract. 



