FAMILY VII. MICROCOCCACEAE 



459 



Gr. adj. philus loving; M.L. adj. cryophilus 

 frost-loving. 



Spherical cells, the average diameter be- 

 ing 1.6 microns, occasional large cells 

 measuring 3.6 and small cells measuring 

 1.0 micron in diameter, occurring singly, in 

 pairs, in chains and in clusters. Nuclear 

 bodies which vary in size and position have 

 been observed in the cell. There is evidence 

 that these bodies represent various stages 

 of a mitotic and a meiotic process (DeLa- 

 mater and Woodburn, Jour. Bact., 64, 1952, 

 793; Hunter, E.xp. Cell Res., 9, 1955, 231). 

 However, compare Bisset (Jour. Bact., 67, 

 1954, 41). Non-motile; occasional cells are 

 motile by means of peritrichous flagella. 

 Gram-stains of veal infusion agar smears 

 show Gram-positive cells with a predomi- 

 nant number of Gram-negative cells irrespec- 

 tive of the age of the culture. 



Gelatin: No liquefaction. 



Agar slant: Creamy white, glistening, fili- 

 form, moderately heavy growth. 



Yeast extract agar: Growth same as 

 above but heavier; old cultures become faint 

 pink in color. 



Veal infusion agar: Growth same as 

 above; old cultures become brownish 

 yellow. 



Yeast e.xtract and nutrient broths : A ring 

 is formed. 



Ulrich milk (Science, 99, 1941, 352) : Alka- 

 line in 2 days; slight reduction of methylene 

 blue indicator in 4 days. 



Indole not produced. 



Hydrogen sulfide not produced. 



No acid from glucose, lactose, sucrose, 

 maltose, xylose, cellobiose, mannitol, dul- 

 citol or salicin. 



Sodium citrate does not serve as a sole 

 source of carbon. 



Nitrites not produced from nitrates. 



Ammonium phosphate does not serve as a 

 sole source of nitrogen. 



Urease is produced. 



Oxidase not produced. 



Blood agar: No hemolysis. 



Catalase-positive. 



Aerobic. 



Temperature relations: Optimum, 9.8° C. 

 Minimum, —4.0° C. Maximum, between 23° 

 and 24° C. 



Optimum pH, between 6.8 and 7.2. Growth 

 occurs between pH 5.5 and 9.5. 



Source: Isolated by McLean (Food Tech- 

 nology, 6, 1951, 7) from a finished package of 

 pork sausage prepared from frozen meat. 



Habitat: Found in frozen meat products 

 so far as known. 



7. Micrococcus conglomeratus Migula, 

 1900. (Citronengelber Diplococcus, Bumm, 

 Der Mikroorganismus der gonorrhoischen 

 Schleimhauterkrankungen, 1 Aufl., 1885, 17; 

 Micrococcus citreus conglomeratus Fliigge, 

 Die Mikroorganismen, 2 Aufl., 1886, 182; 

 Migula, Syst. d. Bakt., 2, 1900, 146.) 



con.glo.me.ra'tus. L. part. adj. conglo- 

 meratus rolled together. 



Spheres, 0.8 to 1.2 microns in diameter, 

 occurring singly, in pairs, in fours and in 

 large clumps. Non-motile. Gram-variable. 



Gelatin colonies: Small, circular, yellow 

 with radiate margin. 



Gelatin stab: Slow crateriform liquefac- 

 tion. 



Agar colonies: Luxuriant, moist, sulfur- 

 yellow. 



Agar slant: Growth light yellow, plumose, 

 slightly rugose, somewhat dull with raised 

 center and transparent margin. 



Broth: Turbid, with light orange ring 

 and sediment. 



Milk: Generally acid but not sufficient 

 to curdle. 



Potato: No growth. 



Indole not produced. 



Acid from glucose and lactose generally, 

 sometimes from sucrose. Mannitol and 

 glycerol generally not fermented. 



Starch not hydrolyzed. 



Nitrites produced from nitrates. 



Ammonia produced from peptone. 



Utilizes NH4H2PO4 as a source of nitro- 

 gen. 



Blood not hemolyzed. 



Non-pathogenic. 



Aerobic. 



Optimum temperature, 25° C. 



Resistant to drying and heat. 



Source: Found in gonorrhoea! pus and in 

 dust. 



Habitat: Infections, milk, dairy products, 

 dairy utensils, water. Common. 



