460 



ORDER IV. EUBACTERIALES 



8. Micrococcus varians Migula, 1900. 

 (Merismopedia flava varians Dyar, Ann. N. 

 Y. Acad. Sci., 8, 1895, 346; Migula, Syst. d. 

 Bakt., 2, 1900, 135.) 



va'ri.ans. L. part. adj. varians varying. 



Spheres, 0.8 to 1.0 micron in diameter, oc- 

 curring singly, in pairs and in fours. Occa- 

 sionally cultures are found that are motile 

 with a single flagellum, otherwise they are 

 non-motile. Gram-variable. 



Gelatin colonies: Small, circular, whitish 

 to yellow, capitate, moruloid. 



Gelatin stab: Scant growth. No liquefac- 

 tion. 



Agar colonies: Small, yellow, raised, 

 glistening. 



Agar slant: Plumose, yellow, variegated 

 growth. 



Broth: Turbid, with yellow, granular 

 sediment. 



Litmus milk: Acid; coagulated on boiling. 



Potato: Raised, dry, bright yellow, glis- 

 tening growth. 



Indole not produced. 



Acid from glucose, lactose, sucrose, raffi- 

 nose and frequently from glycerol and 

 mannitol. No acid from salicin or inulin. 



Starch not hydrolyzed. 



Nitrites produced from nitrates. 



Ammonia produced from peptone. 



Utilizes NH4H2PO4 as a source of nitro- 

 gen. 



Saprophytic. 



Aerobic. 



Optimum temperature, 25° C. 



Source: Original strains found in a con- 

 taminated jar of sterilized milk. 



Habitat: Has been found in body secre- 

 tions, dairy products, dairy utensils, dust 

 and water, including sea water. 



9. Micrococcus caseolyticus Evans, 

 1916. (Jour. Inf. Dis., 18, 1916, 455.) 



ca.se. o.ly'ti.cus. L. noun caseiis cheese; 

 Gr. adj. lyticus able to loose; M.L. adj. 

 caseolyticus casein-dissolving. 



Spheres, variable in size, occurring in 

 clumps. Non-motile. Gram-positive. 



Gelatin stab: Liquefaction generally be- 

 gins after first day and continues rapidly. 



Agar colonies: Yellow to orange (Evans, 

 loc. cit.); pearly white (Hucker, N. Y. Agr. 

 Exp. Sta. Tech. Bull. 102, 1924, 17). 



Agar stroke: Yellow to orange (Evans, op. 

 cit., 18, 1916, 455); pearly white (Hucker, 

 op. cit., 1924, 17); luxuriant growth. 



Broth: Generally grows with smooth tur- 

 bidity although certain strains give heavy 

 precipitate with clear supernatant fluid. 



Litmus milk: Acid, peptonized. Whey 

 generally clear. 



Potato: Scant, white growth. Certain 

 strains may show yellow pigment. 



Indole not produced. 



Acid from glucose, lactose, maltose, man- 

 nitol and glj^cerol. No action on rafiinose. 



Forms dextrorotatory lactic acid (Orla- 

 Jensen, The Lactic Acid Bacteria, 1919, 80). 



Nitrites usually produced from nitrates. 



Asparagin and urea decomposed bj^ some 

 strains. 



Utilizes NH4H2PO4 as a source of nitro- 

 gen. 



Aerobic. 



Optimum temperature, 22° C. 



Saprophytic. 



Source: Eight cultures from a bovine 

 udder. 



Habitat: Milk, dairy utensils and dairy 

 products, especially cheese. 



10. Micrococcus colpogenes Campbell 

 and Williams, 1951. (Jour. Gen. Microbiol., 

 5, 1951, 897 and 904). 



col.po'ge.nes. Gr. noun colpus bosom, 

 fold; Gr. v. gennaio to bear; M.L. adj. 

 colpogenes fold-producing. 



Cocci occurring in clumps and occasion- 

 ally in pairs. Non-motile. Gram-positive. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, smooth, entire, 

 raised, glistening, yellow. 



Agar slant: Filiform, glistening, j^ellow 

 streak. 



Broth: Moderatelj^ turbid; slight, granu- 

 lar sediment. 



Litmus milk: Unchanged. 



Indole not produced. 



Hydrogen sulfide not produced. 



No acid or gas from glucose, lactose, su- 

 crose, maltose, fructose, dextrin, mannose, 

 arabinose, rhamnose, xylose, raffinose, in- 

 ulin, mannitol, salicin, dulcitol, galactose, 

 trehalose, inositol, cellobiose, melezitose or 

 adonitol. Fumarate (0.5 per cent), lactate, 

 malonate, tartrate, citrate, levulinate, 



