462 



ORDER IV. EUBACTERIALES 



Source: Original culture isolated by Buj- 

 wid in Bern, Switzerland, and sent to Mi- 

 gula at Karlsruhe, Germany. 



Habitat: Unknown. 



13. Micrococcus agilis Ali-Cohen, 1889. 

 (Cent. f. Bakt., 6, 1889, 36.) 



a'gi.lis. L. adj. agilis agile. 



Spheres, 1.0 micron in diameter, occurring 

 singly, in pairs and in fours. Motile by 

 means of one or two flagella. Gram- variable. 



Gelatin colonies: Small, gray, becoming 

 distinctly rose-colored. 



Gelatin stab: Thin, whitish growth in 

 stab. On surface thick, rose-red, glistening 

 growth. Generally no liquefaction. 



Agar slant: Growth glistening, dark rose- 

 red, lobed with much variation in color. 



Broth: Slightly turbid, with slight, rose- 

 colored ring and pink sediment. 



Litmus milk : Slightly acid, pink sediment. 



Potato: Slow growth as small, rose- 

 colored colonies. 



Loeffler's blood serum: Pink, spreading, 

 shiny, abundant. Slow liquefaction. 



Indole not produced. 



Acid from glucose, sucrose, inulin, gly- 

 cerol and mannitol. No acid from raffinose. 



Nitrites produced (trace). 



Ammonia formed (trace) . 



Does not utilize NH4H2PO4 as source 

 of nitrogen. 



Aerobic. 



Optimum temperature, 25° C. 



Saprophytic. 



Source: Isolated from water. 



Habitat : Water, sea water and on sea fish. 



14. Micrococcus morrhuae Klebahn, 

 1919. {Micrococcus (DipJococcus) morrhuae 

 Klebahn, Mitteil. a. d. Inst. f. allg. Bot. i. 

 Hamburg, 4, 1919, 47; abst. in Cent. f. Bakt., 

 II Abt., 52, 1921, 123.) 



mor'rhu.ae. M.L. gen. noun morrhuae of 

 Morrhua; M.L. fem.n. Morrhua a genus of 

 codfish. 



Original description supplemented bj' 

 material taken from Better (Proc. Kon. 

 Acad. V. Wetensch. Amsterdam, 34, 1931, 

 1417; also see Petter, Over roode en andere 

 bacterien van gezouten visch. Thesis, 

 Utrecht, 1932) and Elazari-Volcani (Studies 



on the Microflora of the Dead Sea. Thesis, 

 Hebrew Univ., Jerusalem, 1940, V and 65). 



Spheres, 1.0 to 1.5 microns in diameter, 

 occurring singly, in pairs, in short chains 

 and in tetrads. In 30 per cent salt + 1 per 

 cent peptone ("Poulenc"), the spheres are 

 0.9 to 2.7 microns in diameter and occur 

 singly or in pairs or tetrads; in the same 

 agar medium, they appear mostly as cocci, 

 diplococci, streptococci and staphylococci, 

 tetrads being poorly developed. Non-mo- 

 tile. Gram-negative. 



Gelatin stab (15 per cent salt) : Surface 

 growth and liquefaction. 



Agar colonies (24 per cent salt + 1 per 

 cent proteose peptone -f 2 per cent KNO3) : 

 Circular, smooth, entire, raised to conve.x, 

 amorphous, opaque, orange-red. 



Agar slant: Growth filiform, raised, 

 slightly glistening, smooth, Inityrous, 

 opaque, orange-red. 



Broth (24 per cent salt + 1 per cent pro- 

 teose peptone): Slightly turbid; orange-red 

 sediment. 



Indole not produced. 



No acid or gas from arabinose, xylose, 

 glucose, fructose, galactose, mannose, lac- 

 tose, sucrose, maltose, raffinose, inulin, dex- 

 trin, glj'cerol, mannitol or salicin (all tests 

 were made in 24 per cent salt + 1 per cent 

 peptone + 1 per cent carbohydrate during 

 3 weeks). 



Starch not hj^drolj^zed. 



Nitrites produced from nitrates; no gas 

 is produced (tests made in 24 per cent salt 

 -f 1 per cent peptone + 2 per cent KNO3) . 



Catalase-positive. 



Aerobic. 



Salt tolerance : Halophilic, obligate; grows 

 with no morphological changes in 9 to 30 

 per cent salt. When the organism is trans- 

 ferred to water, its morphology does not 

 change, and it grows when reinoculated into 

 salt-containing media. 



Optimum temperature, between 30° and 

 37° C. 



Distinctive character: The pigment pro- 

 duced by this organism gives a blue color 

 with concentrated sulfuric acid, thus sug- 

 gesting a carotenoid; it is not soluble in 

 methanol, ethanol, acetone, ether, chloro- 

 form, dioxam, ethylacetate, benzol, pe- 

 troleum ether, xjdene or pyridine. 



