478 



ORDER IV. EUBACTERIALES 



(Ann. Sci. Nat., Ser. Bot. et Zool., 15, 1933, 

 158). 



Spheres, 0.5 to 0.6 micron in diameter, 

 occurring in pairs and tetrads, rarely in very 

 short chains, never in clu.sters. Gram-posi- 

 tive. 



Gehitin: Good growth. No liquefaction. 



Deep agar colonies: At first very small, 

 lenticular, biconvex, thick, opaque, yel- 

 lowish, 0.5 to 1.5 mm in diameter. Each 

 colony is surrounded by many small satel- 

 lite colonies visible microscopically. 



Broth: Growth slow, poor. After 48 hours 

 a slight, homogeneous turbidity is formed; 

 it quickly clears leaving a slight, powdery 

 sediment. Neither gas nor odor is produced. 



Glucose broth: Growth rapid, abundant. 



Proteins not attacked. 



Blood broth: Good growth; no hemolysis. 



Milk: Poor growth; no change. 



Peptone water: Good growth; not acidi- 

 fied; indole not produced. 



Acid but no gas from glucose and arabi- 

 nose. Slight acid from glycerol. No acid from 

 lactose, inulin, mannitol or dulcitol. 



Neutral red broth: Unchanged. 



Anaerobic. 



Optimum temperature, 37° C. Feeble 

 growth at 22° C. Not thermo-resistant. 



Optimum pH, between 6.0 and 8.0. 



Distinctive character: The microscopic 

 appearance of agar colonies, each of which 

 is surrounded by a constellation of satel- 

 lites. 



Source: Isolated from a case of chronic, 

 cryptic tonsillitis; later isolated from pus 

 in acute appendicitis. 



Habitat: Found in the digestive tract, es- 

 pecially the lymphoid tissues, such as ton- 

 sils, etc. 



8. Peptococcus saccharolyticus (Fou- 

 bert and Douglas, 1948) Douglas, comb. nov. 

 {Micrococcus saccharolyticus Poubert and 

 Douglas, Jour. Bact., 56, 1948, 30 and 31.) 



sac.cha.ro.ly'ti.cus. Gr. noun sacchar 

 sugar; Gr. adj. lyticus able to loose; M.L. 

 adj . saccharolyticus sugar-digesting. 



Spheres, 0.6 to 1.0 micron in diameter, 

 occurring singly, in pairs, tetrads and ir- 

 regular groups. Non-motile. Not encapsu- 

 lated. Gram-positive. 



Gelatin: No liquefaction. 



Agar colonies: Circular, 0.5 to 1.0 mm in 

 diameter, smooth, entire, low convex, 

 opaque, grayish white, butyrous. 



Growth in fluid medium: In peptone yeast 

 extract broth, growth is moderate and 

 stimulated by glucose. Cultures are turbid 

 with a white, powdery sediment. No visible 

 gas production. Faint, fruity odor. 



Litmus milk: Slight reduction of litmus. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no visible gas from glucose, 

 fructose, mannose and glycerol. Arabinose, 

 galactose, maltose, lactose, raffinose, starch, 

 inulin, salicin, mannitol, lactate and malate 

 not utilized. Glucose fermented to CO2 , 

 ethanol, acetic acid, formic acid and traces 

 of lactic acid. 



Nitrites produced from nitrates; nitrites 

 are reduced. 



Egg albumen, beef serum and casein not 

 attacked. 



Coagulase-negative. 



Catalase-positive. 



Anaerobic. 



Optimum temperature, 37° C. Growth 

 between 25° and 37° C. 



Optimum pH, between 7.0 and 7.5; pH 

 range, 5.5 to 8.5. 



Non-hemolytic. 



Distinctive characters: Saccharolytic; no 

 visible gas produced; characteristic fer- 

 mentation products from glucose. 



Comments: There seems to be little dif- 

 ference between this and the preceding 

 species. 



Source: Isolated from bottles of plasma; 

 also from the human skin. 



Habitat: Probably the skin of man. 



9. Peptococcus glycinophilus (Cardon 

 and Barker, 1946) Douglas, comb. nov. 

 {Diplococcus glycinophilus Cardon and 

 Barker, Jour. Bact., 52, 1946, 629.) 



gly.ci.no'phi.lus. M.L. noun glycinum 

 glycine; Gr. adj. philus loving; M.L. adj. 

 glycinophilus glycine-loving. 



Original description supplemented by 

 material from Foubert (Thesis, University 

 of Washington, 1947). 



Spheres, 0.7 to 2.5 microns in diameter, 

 the average being about 1.2 microns, occa- 

 sionally rod-shaped, occurring in pairs, 



