492 ORDER IV. EUBACTERIALES 



3. Nitrite production not recorded. 



17. Brevibacierium healii. 

 II. Motile. 



A. Gelatin not liquefied. 



1. Grayish white on agar slant. 



18. Brevibacierium incertum. 



2. Pinkish orange to j'ellow on agar slant. 



19. Brevibacierium imferiale. 



B. Gelatin liquefied. 



1. Grayish white on agar slant. 



20. Brevibacierium lipolylicum. 



2. Yellow to yellow-brown on agar slant. 



a. Orange chromogenesis on potato. 



21. Brevibacierium acelylicum. 

 aa. Yellow chromogenesis on potato. 



b. Sulfur-yellow streak on potato. 



22. Brevibacierium sulftireum. 

 bb. Abundant, thickened, yellow growth on potato. 



23. Brevibacierium helvolum. 



1. Brevibacierium linens (Weigmann, 

 1910) Breed, 1953. (Organismus IX, Wolff, 

 Milchwirt. Zent., 5, 1909, 145; Baclerium 

 linens Weigmann, in Wolff, Cent. f. Bakt., 

 II Abt., 28, 1910, 422, and in Weigmann, 

 Mykologie der Milch, 62, 1911, 220; Breed, 

 Riassunti delle Comunicazioni, VI Con- 

 gresso Internaz. di Microbiol., Roma, 1, 

 1953, 13; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 13.) 



li'nens. L. part. adj. linens spreading over, 

 smearing. 



Also see Steinfatt (Milchwirt. Forsch., 9, 

 1930, 7), Kelly (Jour. Dairy Sci., 20, 1937, 

 239) and Albert, Long and Hammer (Iowa 

 Agr. Exp. Sta. Res. Bui. 328, 1944). 



Rods. Average 0.6 by 2.5 microns when 

 grown 1 to 2 days on tryptone glucose ex- 

 tract agar. Non-motile (Wolff, op. cit., 

 1909, 145). Gram-positive (Kelly, op. cit., 

 1937, 239). 



Gelatin colonies: At 18° C., punctiform at 

 first; after 12 daj^s about 1 mm in diameter, 

 compact, circular, shiny, brownish yellow to 

 red-brown. Liquefaction. 



Gelatin stab: At 21° C., crateriform li- 

 quefaction, becoming infundibuliform on 

 extended incubation. Rate of liquefaction 

 varies considerably with different cultures, 

 some completing it in 15 days, others not 

 completing it even on long incubation. 



Agar colonies: On tryptone glucose ex- 



tract agar at 21° C. after 1 to 2 days, colonies 

 convex, glistening, entire and cream- 

 colored, becoming brown on extended in- 

 cubation; diameters 2 to 5 mm. On special 

 cheese agar with incubation in oxygen, 

 luxuriant growth, the color becoming bright 

 orange to reddish brown in 4 or 5 days. 



Agar stab: Heavy surface growth on 

 tryptone glucose extract agar at 21° C. with 

 no growth along the line of inoculation. 



Agar slant: On tryptone glucose extract 

 agar at 21° C. after 2 days, growth abun- 

 dant, glistening, filiform, non-viscid and 

 cream-colored. After extended incubation 

 the color usually is brown. On special cheese 

 agar in an atmosphere of oxygen, the growth 

 is bright orange to reddish brown in 4 or 5 

 days. 



Broth: Turbidity and sediment. 



Potato: At 21° C. after 5 days, growth is 

 scant, smooth, glistening and varies in 

 color from grayish to brownish orange. 



Litmus milk: At 21° C. the changes are 

 very slow. After 6 or 7 days the reaction be- 

 comes alkaline and a yellow sediment ap- 

 pears. After approximately 10 days some 

 digestion is evident, complete digestion gen- 

 erally requiring several weeks to over a 

 month. A distinct ammoniacal odor, more 

 or less objectionable, produced in old cul- 

 tures. No coagulation. Ropiness often pro- 

 duced on extended incubation. 



