FAMILY IX. BREVIBACTERIACEAE 



497 



Source: Isolated from a triturated speci- 

 men of the mud-dauber wasp, Sceliphron 

 cementariiim Dru. 



Habitat: Unknown. 



10. Brevibacteriuni sociovivuni (ZoBell 

 and Upham, 1944) Breed, 1953. {Bacterium 

 sociovivnm ZoBell and Upham, Bull. Scripps 

 Inst. Oceanography, La Jolla, 5, 1944, 269; 

 Breed, Riassunti delle Comunicazioni, VI 

 Congresso Internaz. di Microbiol., Roma, 1, 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 14.) 



so.ci.o.vi'vum. L. noun socius com- 

 panion, partner; L. v. vivo to live; M.L. adj. 

 sociovivus partner living. 



Rods, 0.5 to 0.8 by 3.0 to 4.0 microns, 

 with rounded ends, occurring singly, inpairs 

 and in chains. Non-motile. Gram-positive, 

 but tends to destain, leaving Gram-positive 

 cell wall and granules. 



All differential media except the fresh- 

 water broth, litmus milk and potato were 

 prepared with sea water. 



Gelatin colonies: Irregular, sunken, gray- 

 ish white; filamentous margin. 



Gelatin stab: Crateriform liquefaction 

 becoming stratiform. 



Agar colonies: 2 to 4 mm in diameter, 

 circular, convex, smooth, entire, darker 

 center. 



Agar slant: Luxuriant, beaded, glisten- 

 ing, butyrous growth with no pigment. 



Sea-water broth: No pellicle; no turbid- 

 ity; heavy, flocculent sediment. 



Fresh-water broth: Fair growth. 



Litmus milk: Decolorized; neutral; com- 

 pletely peptonized in 20 days. 



Potato: Abundant, dull, light cream- 

 colored growth. Potato darkened. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, maltose and 

 mannitol. No acid from glycerol, lactose, 

 sucrose or salicin. 



Starch is hydrolyzed. 



Non-lipolytic. 



Nitrites not produced from nitrates. 



Ammonia produced from peptone but not 

 from urea. 



Casein is digested. 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 20° and 

 25° C. 



Source: Found associated with sedentary 

 organisms in the sea. 



Habitat: Commonly found on submerged 

 surfaces and on sessile diatoms in sea water. 



11. Brevibacteriuni iinmotuin (ZoBell 



and Upham, 1944) Breed, 1953. {Bacterium 

 immotum ZoBell and Upham, Bull. Scripps 

 Inst. Oceanography, 5, 1944, 271; Breed, 

 Riassunti delle Comunicazioni, VI Con- 

 gresso Internaz. di Microbiol., Roma, 1, 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 14.) 



im.mo'tum. L. adj. immotus motionless. 



Rods, 0.8 by 3.1 to 8.6 microns, with 

 rounded ends, occurring singly, in pairs and 

 in long chains. Non-motile. Gram-positive, 

 but tends to destain, leaving Gram-positive 

 outline and granules. 



All differential media except the fresh- 

 water broth, litmus milk and potato were 

 prepared with sea water. 



Gelatin colonies: Small, circular, raised, 

 gray, slowly digest gelatin. 



Gelatin stab : Crateriform liquefaction be- 

 coming infundibuliform. Beaded growth 

 along line of stab. No pigment. 



Agar colonies: 1 to 2 mm in diameter, 

 circular, convex, smooth, lobate margin, 

 darker centers. 



Agar slant: Luxuriant, glistening, echinu- 

 late, mucoid growth with no pigment. 



Sea-water broth: No pellicle; moderate 

 turbidity; abundant, flocculent sediment. 



Fresh-water broth: Scant growth. 



Litmus milk: Decolorized; neutral; partly 

 peptonized in 20 days. 



Potato: Luxuriant, mucoid, cream}^ 

 growth which darkens potato. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, maltose, 

 xylose and mannitol. No acid from glycerol, 

 lactose, sucrose or salicin. 



Starch is hydrolyzed. 



Non-lipolytic. 



Nitrites not produced from nitrates. 



Ammonia produced from peptone but not 

 from urea. 



Casein is digested. 



