FAMILY IX. BREVIBACTERIACEAE 



499 



which is fixed in position, a station; L. gen. 

 noun slationis of a fixed position. 



Ovoid rods, 0.4 by 0.5 to 0.6 micron, 

 occurring singly or in chains of two to three. 

 Non-motile. Gram-positive, but easily de- 

 stained. 



All media except the fresh-water broth, 

 litmus milk and potato were prepared with 

 sea water. 



Gelatin colonies: 0.5 to 1.0 mm in diame- 

 ter, circular, convex, grayish white. 



Gelatin stab: Very slow, napiform lique- 

 faction. 



Agar colonies: 1 to 2 mm in diameter, 

 convex, smooth, colorless; lobate edge. 



Agar slant: Moderate, glistening, filiform, 

 butyrous growth with no pigment. 



Sea-water broth: Heavy pellicle; no tur- 

 bidity; granular growth along walls; scant 

 sediment. 



Fresh-water broth: Good growth. 



Litmus milk: Becomes alkaline. Casein 

 not digested. 



Potato: No visible growth. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose. Lactose, 

 maltose, sucrose, mannitol, glycerol, xylose 

 and salicin not utilized. 



Starch not hydrolyzed. 



Non-lipolytic. 



Of 19 amino acids tested, none was re- 

 quired for growth; preformed growth factors 

 also were not required (Campbell and Wil- 

 liams, Food Research, 16, 1951a, 506). 



Ammonium chloride and the 19 amino 

 acids which were tested may serve as sources 

 of nitrogen; the amino acids may also be 

 utilized as carbon sources (Campbell and 

 Williams, loc. cit.). 



Nitrites rapidly produced from nitrates. 



Ammonia produced from peptone but not 

 from urea. 



Trimethylamine not produced from tri- 

 methjiamine oxide, betaine, choline or 

 acetyl choline (Campbell and Williams, 

 Jour. Bact., 62, 1951b, 250). 



Inorganic sulfur may serve as a source of 

 sulfur (Campbell and Williams, op. cit., 

 1951a, 506). 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 20° and 

 25° C. 



Source: Isolated from a film of marine 

 fouling organisms. 

 Habitat: Sea water. 



15. Brevibacterium quale (Steinhaus, 

 1941) Breed, 1953. (Bacterium qualis (sic) 

 Steinhaus, Jour. Bact., 42, 1941, 774; Breed, 

 Riassunti delle Comunicazioni, VI Con- 

 gresso Internaz. di Microbiol., Roma, 1, 

 1953, 13; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 13.) 



qua'le. L. proun.adj. qualis of what sort? 



Short rods, very short on solid media, 

 frequently ellipsoidal in shape. In fluid 

 media, 0.5 to 0.7 by 1.4 to 2.2 microns, 

 occurring singly. Non-motile. Gram-posi- 

 tive. 



Gelatin stab: Liquefaction. 



Agar colonies: Small (1 mm), white, glis- 

 tening, transparent, circular, entire. 



Agar slant: Filiform, smooth, glistening. 



Broth: Almost clear; slight turbidity in 

 serum and glucose broth. 



Litmus milk: No change. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose, sucrose and maltose. 

 Lactose not fermented. 



Starch not hydrolyzed. 



Slight production of nitrites from ni- 

 trates. 



Source: Isolated from the alimentary 

 tract of the tarnished plant bug, Lygus 

 pratensis L. 



Habitat: Unknown. 



16. Brevibacterium animoniagenes 



(Cooke and Keith, 1927) Breed, 1953. (Bac- 

 terium ammoniagenes Cooke and Keith, 

 Jour. Bact., 13, 1927, 315; Breed, Riassunti 

 delle Comunicazioni, VI Congresso Inter- 

 naz. di Microbiol., Roma, 1, 1953, 14; also 

 see Atti del VI Congresso Internaz. di 

 Microbiol., Roma, 1, 1955, 14.) 



am.mo.ni.a'gen.es. M.L. noun ammonia 

 ammonia, from Gr. noun Amnion (Jupiter), 

 worshipped in Egypt in the form of a ram; 

 Gr. V. gennaio to produce; M.L. adj. am- 

 moniagenes ammonia producing. 



Rods with rounded ends, 0.8 by 1.4 to 1.7 

 microns, occurring singly. Not encapsu- 

 lated. Non-motile. Gram-positive. 



Gelatin stab: No liquefaction. 



