500 



ORDER IV. EUBACTERIALES 



Agar colonies: Circular, flat, smooth, en- 

 tire, gray. Occasionally a faint yellow 

 chromogenesis is produced. 



Agar slant: Growth moderate, smooth, 

 flat, opaque, glistening, butyrous, amor- 

 phous. No odor. 



Agar stab: Filiform growth more abun- 

 dant near the surface. 



Broth: Moderate turbidity near the sur- 

 face; flocculent sediment. 



Litmus milk: Slightly alkaline. 



Indole not produced. 



No action on carbohydrates. 



Nitrites produced from nitrates (personal 

 communication, test of A.T.C.C. cultures, 

 numbers 6871 and 6872, by W. A. Clark, 

 1954). 



Urea is fermented forming ammonia. 



Blood serum not liquefied. 



Aerobic, facultatively anaerobic. 



Optimum temperature, 30° C. Killed at 

 55° C. in ten minutes. 



Optimum pH, between 7.0 and 8.5; does 

 not grow readily in media with more acid or 

 more alkaline reaction than this. 



Not pathogenic for rabbits or guinea pigs. 



Source: Isolated from the feces of infants. 

 Cause of a diaper rash of infants. 



Habitat: Presumably widely distributed 

 in putrefjdng materials. 



17. Brevibacterium healii (Buchanan 

 and Hammer, 1915) Breed, 1953. (Bacterium 

 healii Buchanan and Hammer, Iowa Agr. 

 E.xp. Sta. Research Bull. 22, 1915, 249; 

 Breed, Riassunti delle Comunicazioni, VI 

 Congresso Internaz. di Microbiol., Roma, 1, 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 14.) 



hea'li.i. M.L. gen. noun healii of Healy; 

 named for Dr. Healy. 



Rods 0.5 to 0.7 by 2.2 to 12.9 microns; 

 chains and filaments common. Not encap- 

 sulated. Non-motile. Gram-positive. 



Produces a slight flocculent growth in 

 Uschinsky's solution. 



Gelatin stab: Heavy growth with strati- 

 form liquefaction beginning at surface. 

 Villous to arborescent growth along the 

 stab. 



Agar stab: Heavy white, brittle surface 

 growth. Villous to arborescent growth 

 along line of inoculation. 



Agar streak: White, hard, arborescent 

 growth; no tendency to stringiness. 



Agar colonies: Large, white, rhizoid. 



Broth: No turbidity; forms a pellicle 

 which sinks either entirely or in part. 



Litmus milk: Slightly acid, becoming 

 slimy, coagulated and peptonized. 



Potato: Heavy white, shiny, non-viscous 

 growth. 



Indole not produced. 



Acid but no gas from glucose, fructose, 

 maltose, sucrose, salicin and starch. No acid 

 from mannitol, lactose, raffinose or inulin. 



Grows well at room temperature. Does 

 not survive 80° C. for 5 minutes. 



Distinctive character: Morphologically 

 this species resembles the species in Kurthia; 

 further study may show that it should be 

 transferred from Brevibacterium to Kurthia. 



Source: Isolated from bitter and slimj^ 

 milk by Dr. Healj^ of Kentucky; also from 

 dairy products by Esten. 



Habitat : Dairy products, and presumably 

 widely distributed in decomposing organic 

 materials. 



18. Brevibacteriiim incertum (Stein- 

 haus, 1941) Breed, 1953. (Bacterium incertum 

 Steinhaus, Jour. Bact., 42, 1941, 776; Breed, 

 Riassunti delle Comunicazioni, VI Con- 

 gresso Internaz. di Microbiol., Roma, 1, 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 13.) 



in.cer'tum. L. adj. incertus uncertain. 



Short rods, 0.5 to 0.8 by 1.0 to 1.5 microns, 

 occurring singly and occasionally in pairs. 

 Young cultures motile. Monotrichous 

 (Steinhaus, personal communication, 1955). 

 After 48 hours generally non-motile. Gram- 

 positive; after 48 hours many cells become 

 Gram-negative. 



Gelatin stab: No liquefaction. 



Agar colonies: Tiny, grayish white, 

 smooth, almost transparent. Does not grow 

 well on nutrient agar. 



North's gelatin chocolate agar slant: Fili- 

 form, thin, transparent growth. Brown color 

 of chocolate medium changes to yellowish 

 green. 



Blood agar: Alpha hemolysis at first; after 

 three days, beta hemolysis. 



Broth: Almost clear; very slight growth. 



Litmus milk: No change. 



