FAMILY IX. BREVIBACTERIACEAE 



501 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, sucrose, 

 fructose, mannose and maltose. No fermen- 

 tation of lactose, rhamnose, galactose, 

 mannitol, dulcitol, inositol or sorbitol. 



Acetylmethylcarbinol not produced. 



Starch not hydrolyzed. 



Nitrites not produced from nitrates. 



Microaerophilic. 



Source: Isolated from the ovaries of the 

 lyreman cicada, Tihicen linnet Smith and 

 Grossbeck. 



Habitat: Unknown. 



19. Brevibacteriuiti imperiale (Stein- 

 haus, 1941) Breed, 1953. {Bacterium im- 

 periale Steinhaus, Jour. Bact., 42, 1941, 777; 

 Breed, Riassunti delle Comunicazioni, VI 

 Congresso Internaz. di Microbiol., Roma, 1 , 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, /, 1955, 13.) 



im.pe.ri.al'e. L. adj. imperialis imperial; 

 from specific epithet of name of insect host. 



Small rods, 0.5 to 0.8 by 1.0 to 1.7 microns, 

 occurring singly and in pairs. 



A few cells are motile in young cultures. 

 Monotrichous; occasionally the flagellum is 

 lateral (Steinhaus, personal communication, 

 1955). Gram-positive. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, entire, almost 

 translucent, pinkish orange to yellow pig- 

 ment. 



Agar slant: Filiform, glistening, opaque 

 growth. 



Broth: Slight to moderate turbidity; 

 slight sediment. 



Litmus milk: No change at first, later 

 slightly acid. 



Potato: Heavy, glistening, moist growth; 

 reddish to yellowish orange. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, sucrose, 

 maltose, fructose, mannitol, galactose, 

 arabinose, xylose, salicin, raffinose, tre- 

 halose, sorbitol, mannose, adonitol and 

 esculin, and slight acid from lactose and 

 dextrin. Inulin, dulcitol, glycerol, rham- 

 nose, adonitol and inositol not fermented. 



Starch not hydrolyzed. 



Nitrites not produced from nitrates. 



Aerobic. 



Source: Isolated from the alimentary 

 tract of the imperial moth, Eacles imperialis 

 Dru. 



Habitat: Unknown. 



20. Brevibacteriiiin lipolylicuin (Huss, 

 1908) Breed, 1953. (Bactridiutn lipolyticum 

 Huss, Cent. f. Bakt., II Abt., 20, 1908, 474; 

 Breed, Riassunti delle Communicazioni, VI 

 Congresso Internaz. di Microbiol., Roma, /, 

 1953, 14; also see Atti del VI Congresso 

 Internaz. di Microbiol., Roma, 1, 1955, 14.) 



li.po.ly'ti.cum. Gr. noun lipus animal 

 fat; Gr. adj. lyticus dissolving; M.L. adj. 

 lipolyticus fat-dissolving. 



Small, coccoid rods, 0.3 to 0.5 by 0.7 to 

 1.4 microns, occasionally as long as 3.5 mi- 

 crons. Cells coccoid on gelatin media, form- 

 ing chains resembling those of the strepto- 

 cocci. Motile by means of peritrichous 

 flagella. Gram-positive. 



Gelatin colonies: Circular, grayish to 

 transparent with irregular margin. 



Gelatin stab: Infundibuliform liquefac- 

 tion. 



Agar colonies: Circular, gray, smooth, 

 butyrous, glistening, entire. Old colonies 

 brownish yellow. 



Whey agar: Growth on surface and along 

 the stab; surface growth maj^ become 

 faintly yellowish. 



Whey: Heavy turbidity at 20° C; forms a 

 heavy precipitate after 8 days. 



Broth: Turbid; granular sediment. 



Litmus milk: Coagulated, peptonized and 

 becoming alkaline. 



Potato: Moist, glistening, yellowish white 

 growth. 



Acid from glucose, sucrose, raffinose, xy- 

 lose, mannitol and glycerol. Lactose not 

 fermented. 



Indole reaction faint. 



Hydrogen sulfide not produced. 



Fats are split in milk, giving rise to a 

 rancid odor and a bitter taste. 



Reduction of nitrates questionable. 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, 35° C. 

 Growth at 14° but not above 45° C. 



Comments: This species is definitely not 

 Bacterium lipolyticum Evans (Jour. Inf. 

 Dis., 22, 1918, 576). The latter organism is a 



