528 



ORDER IV. EUBACTERIALES 



Sodium hippurate not hydrolyzed. 

 Source: Normal human throat. Not con- 

 sidered to be pathogenic. 

 Habitat: Human throat. 



4. Streptococcus sp. Fry. (Group L, Hare 

 and Fry, Vet. Rec, 1, 1938, 1537.) Addi- 

 tional information from Laughton, Jour. 

 Path. Bact., 60, 1948, 471. 



Spherical or ovoid cells in long chains. 

 Gram-positive. 



Serology: Established as group L. 



Action on blood: Surface and submerged 

 colonies are beta hemolytic. Most strains 

 do not produce a demonstrable soluble 

 hemolysin. 



Colony forms: Glossy, matt and inter- 

 mediate types observed on blood agar. 



Final pH in glucose broth, between 4.7 

 and 5.2. 



Acid produced from maltose, lactose, 

 sucrose, trehalose and salicin. May or may 

 not ferment glycerol and sorbitol. 



Sodium hippurate not hydrolyzed. 



Source: Isolated from miscellaneous in- 

 fections of the dog. 



Habitat : Probably the throat and genital 

 tract of the dog. 



5. Streptococcus sp. Fry. (Group M, Hare 

 and Fry, Vet. Rec, 1, 1938, 1537.) Addi- 

 tional information from Laughton, Jour. 

 Path. Bact., 60, 1948, 471. 



Spherical or ovoid cells in long chains. 

 Gram-positive. 



Serology: Established as group M. At 

 least two serological types are known. 



Action on blood: Surface and submerged 

 colonies are beta hemolytic. A weak, slow 

 acting soluble hemolysin produced by some 

 strains; other strains appear to be negative. 



Colony forms: Glossy, matt and inter- 

 mediate types observed on blood agar. 



Final pH in glucose broth, between 4.6 

 and 6.9. 



Acid produced from maltose, lactose, 

 sucrose and usually from trehalose. May or 

 may not ferment glycerol and salicin. Sor- 

 bitol not fermented. 



Sodium hippurate not hydrolyzed. 



Source: Isolated from the urethra, the 

 vagina and the tonsillar area of dogs. Prob- 



ably not associated with any canine dis- 

 ease. 

 Habitat: Probably the dog. 



6. Streptococcus sp. Boissard and Wor- 

 mald. (Hitherto undescribed serological 

 group O, Boissard and Wormald, Jour. 

 Path. Bact., 62, 1950, 37.) 



Spherical or ovoid cells occurring in very 

 long chains in broth culture. 



Serology: Belongs to a new serological 

 group for which "group O" is proposed. 



Action on blood: Hemolysis on blood agar 

 ranges from frank green to a moderately 

 wide zone of beta hemolysis similar to that 

 produced hy Streptococcus pyogenes. Growth 

 under anaerobic conditions reduces or com- 

 pletely inhibits the beta hemolysis. No 

 soluble hemolysin detected. 



Colonj^ forms: Surface colonies on blood 

 agar 0.4 to 0.8 mm in diameter after 18 hours 

 at 37° C. with flattened margin and raised 

 center. The margin is radically striated and 

 has a beaded or pleated edge. Colony has 

 rubbery and coherent consistency. 



Not fibrinolytic. 



Temperature relations: Does not survive 

 60° C. for 30 minutes. 



Tolerance tests: May or may not grow 

 on blood agar containing 10 per cent bile. 

 No growth on 40 per cent bile blood agar. 



Final pH in glucose broth, between 4.5 

 and 5.1. 



Acid produced from glucose and lactose. 

 May or maj^ not ferment trehalose and sali- 

 cin. No acid from mannitol or sorbitol. 



Sodium hippurate not hj'drolyzed. 



Not pathogenic for mice. 



Source: Nasopharynx of normal humans; 

 occasionally from throats of individuals 

 suffering from tonsilitis. 



Habitat: Probably the human naso- 

 pharynx. 



7. Streptococcus MG. Mirick, Thomas, 

 Curnen and Horsfall. (Jour. Exp. Med., 80, 

 1944, .391, 407 and 431.) 



Spherical or ovoid cells occurring in pairs 

 or short chains. Gram-positive. 



Serology: No group-specific antigen has 

 been demonstrated. All strains belong to one 

 serological type due to the presence of a 

 type-specific capsular antigen shown to be 



