FAMILY X. LACTOBACILLACEAE 



537 



and traces of lactic acid and acetylmethyl- 

 carbinol. 



Neutral red reduced. 



Nitrites not produced from nitrates. 



Anaerobic. 



Optimum temperature, between 30° and 

 37° C. 



Optimum pH, between 6.5 and 8.2. 



Non-pathogenic for guinea pigs, rabbits 

 and mice. 



Comments: A hemolj'tic variety of this 

 organism has been reported by Beeuwkes 

 and Aladame (Ann. Inst. Past., 75, 1948, 

 390). 



Source: Isolated from a subacute case of 

 pulmonary gangrene. 



Habitat : Found in the natural cavities of 

 man, especially the respiratory. 



5. Peptostreplococcus lanceolatu.s 



(Prevot, 1933) Smith, comb. nov. {Coccus 

 lanceolatus anaerobhis Tissier, Compt. rend. 

 Soc. Biol., Paris, 94, 1926, 447; Streptococcals 

 lanceolatus Prevot, Ann. Sci. Nat., Ser. 

 Hot., 15, 1933, 173 and 193; not Streptococcus 

 lanceolatus Gamaleia, Ann. Inst. Past., 2, 

 1888, 440.) 



lan.ce.o.la'tus. L. adj. lanceolatus lancet- 

 shaped. 



Large, ovoid cells, 1.2 to 1.4 microns in 

 diameter, with pointed ends, occurring in 

 short chains in culture and in pairs in ex- 

 udates. Non-motile. Gram-positive. 



Gelatin: No liquefaction. 



Deep agar colonies: Very large, lenticular; 

 abundant gas produced which breaks up 

 the medium. 



Peptone broth: Uniform turbidity; gran- 

 ular, viscous sediment. 



Peptone broth: Good growth; gas pro- 

 duced. 



Milk: No change. 



Coagulated protein not attacked. 



Hydrolyzed albumen reduced to CO3 , 

 (NH4)2C03 and NH,,. 



Acid from glucose, sucrose and starch. 

 No acid from lactose. Butyric, valerianic 

 and acetic acids are produced, in the pro- 

 portions 2:1: trace, from glucose and su- 

 crose. 



Anaerobic. 



Optimum temperature, 37° C. 



Non-pathogenic for laboratory animals. 



Distinctive characters: Proteolytic and 

 saccharolytic; produces ammonia from 

 hydrolyzed proteins; butyric, valerianic 

 and acetic acids are produced from certain 

 carbohydrates. No hydrogen sulfide is pro- 

 duced. 



Source: Isolated from human feces in a 

 case of diarrhoea. 



Habitat: Found in putrefying materials. 



6. Peptostreptococcus micros (Prevot, 

 1933) Smith, comb. nov. {Streptococcus an- 

 aerobius micros Lewkowicz, Arch. Med. Exp., 

 IS, 1901, 645; Streptococcus micros Prevot, 

 Ann. Sci. Nat., Ser. Bot., 15, 1933, 193.) 



mi'cros. Gr. adj. micrus small. 



Very small spheres, 0.2 to 0.4 micron in 

 diameter, occurring in long chains or in 

 pairs. Non-motile. Gram-positive. 



Gelatin: Poor growth. No liquefaction. 



Semi-solid agar (Veillon) : Slow growth; 

 colonies at first punctiform, becoming len- 

 ticular and later forming processes into the 

 medium. Average size, 0.5 to 1.0 mm in di- 

 ameter; some reach 2 to 3 mm, growing 2 or 

 3 cm below the surface. 



Blood agar: No hemolysis. No hemopep- 

 tolysis. 



Martin broth: Sliglit, particulate tur- 

 bidity which slowly settles. 



Meat and liver broth: Rapid growth; 

 abundant sediment. 



Peptone broth: Powdery sediment; me- 

 dium not acidified; no indole is produced. 



Milk: Grows with difficulty; no acid; no 

 coagulation. 



Coagulated protein not attacked. 



Acid from glucose, fructose, galactose, 

 sucrose and maltose. Fermentation prod- 

 ucts include propionic, formic and lactic 

 acids (Pr(5vot, Man. d. Classif. et d. De- 

 term, d. Bact. Anaerobies, 2" ed., 1948, 59). 



Neutral red broth is changed to fluores- 

 cent yellow. 



Anaerobic. 



Optimum temperature, between 36° and 

 38° C. No growth at 22° C. Killed in 15 min- 

 utes at 60° C. 



Optimum pH, about 7.0. 



Non-pathogenic for mice. 



No toxin and no hemolysin. 



Distinctive characters: Neither gas nor 

 fetid odor produced; small size. 



