540 



ORDER IV. EUBACTERIALES 



Temperature relations: Optimum, 37° C. 

 Grows from 18° to 37° C. Killed in five 

 minutes on boiling orinhalf an hour at 60° C. 



Optimum pH, 7.0. Limits of pH, 5.5 to 

 8.5 



Non-pathogenic. 



Distinctive characters: Large size; very 

 marked alkalinizing power. 



Source: Isolated from putrefying butch- 

 er's meat (Tissier and Martelly) and from 

 a case of acute appendicitis (Prevot). 



Habitat: Unknown. 



11. Peptostreptococcus paleopneutno- 

 niae (Prevot, 1930) Smith, comb. nov. (An 

 anaerobic pseudopneumococcus, Etudes 

 bact. sur les infections d'origine otique, 

 Rist, These med., Paris, 1898; also see 

 Compt. rend. Soc. Biol., Paris, 52, 1902, 

 305; Der Frankelsche Diplococcus, Bolog- 

 nesi, Cent. f. Bakt., I Abt., Orig., 43, 1907, 

 113; Diplococcus paleopneutnoniae Prevot, 

 Ann. Sci. Nat., Ser. Bot., 15, 1933, 143.) 



pa.le.o.pneu.mo'ni.ae. Gr. adj. palaeus 

 old; Gr. noun pneumon the lungs; M.L. noun 

 pneumonia pneumonia; M.L. fem.gen.n. 

 paleopneumoniae of old pneumonia. 



Spheres, about 0.7 to 1.0 micron in di- 

 ameter, occurring in pairs, rareh' singly or 

 in very short chains. Encapsulated. Gram- 

 positive. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. 



Blood agar colonies: Round, raised, 

 transparent, dew drop. No hemolysis. 



Broth : Opalescent turbidity which settles 

 as a rather abundant, powdery, flocculent 

 precipitate. No gas produced. 



Glucose or lactose broth: Rapid, abun- 

 dant growth. 



Peptone broth (2 per cent) : Very slow 

 development; after 4 or 5 days at 37° C., 

 growth very poor. 



Milk: Good growth; partial coagulation. 



Blood agar: Very rapid, abundant growth. 



Coagulated protein not attacked. 



Acid from glucose and lactose. 



Anaerobic. Some strains become aero- 

 tolerant (Smith, Brit. Jour. Exp. Path., 17, 

 1936, 329). 



Temperature relations: Optimum, 37° C. 

 No growth at 20° nor at 42° C. Killed at 

 55° C. 



Pathogenic for mice. 



Distinctive characters: Resembles Di- 

 plococcus pneumoniae but is a strict anaer- 

 obe; highly pathogenic. 



Source : Isolated from an osseous abscess 

 (Rist), from lesions of pleuropneumonia 

 (Bolognesi) and from bronchitis and pneu- 

 monia (Smith). 



Habitat: Found in the buccal-pharyngeal 

 cavity of man and rodents. 



12. Peptostreptococcus plagarunibelli 



(Prevot, 1933) Smith, comb. nov. {Diplococcus 

 from septic wounds, Adamson, Jour. Path. 

 Bact., 22, 1919, 393; Diplococcus plagarum- 

 belli Prevot, Ann. Sci. Nat., Ser. Bot., 15, 

 1933, 157.) 



pla.ga.rum.bel'li. L. noun plaga injury, 

 plague; L. noun bellum war; M.L. gen. noun 

 plagarumbelli of the plagues of war. 



Spheres, 0.6 to 1.0 micron in diameter, 

 occurring in pairs of unequal size or in short 

 chains. Gram-positive. 



Gelatin: No liquefaction. 



Deep agar colonies: Appear after 24 to 

 48 hours, gradually increasing in size to 

 to 2 mm in diameter; lenticular, regular, 

 almost transparent. Gas is not produced, 

 even in glucose agar. 



Broth : Growth precipitates in 5 or 6 days; 

 no gas is produced. 



Milk: Strongly acidified and coagulated in 

 2 to 3 days. 



Coagulated proteins not digested. 



Indole not produced. 



Acid but no gas from glucose, maltose, 

 lactose and sucrose. 



Anaerobic. 



Temperature relations: Optimum, 37° C. 

 Not always killed in 30 minutes at 80° C. 



Non-pathogenic. 



Source: Sixteen strains were isolated from 

 fifty-one cases of septic war wounds. 



Habitat: Common in septic wounds. 



13. Peptostreptococcus niorbilloruni 



(Prevot, 1933) Smith, conib. nov. (Diplo- 

 cocci from cases of measles, Tunnicliff, 

 Jour. Amer. Med. Assoc, 68, 1917, 1028; 

 Diplococcus rubeolae Tunnicliff, Jour. Inf. 

 Dis., 52, 1933, 39; Diplococcus morbilloruju 

 Prevot, Ann. Sci. Nat., S^r. Bot., 15, 1933, 



