FAMILY X. LACTOBACILLACEAE 



555 



Jtl, 1902, 474; Pr6vot, Ann. Inst. Past., 60, 

 1938, 294.) 



quar'tum. L. adj. quartus fourth. 



Thick rods, with rounded ends, which 

 vary in length. Non-motile. Gram-positive. 



Gas and fetid odor produced in culture 

 media. 



Gelatin: Liquefaction in 6 to 9 days. Sedi- 

 ment. 



Deep agar colonies : Small, round, arbores- 

 cent. 



Glucose broth: Turbid. Abundant gas. 

 Sediment. 



Milk: Digested in 3 to 5 days. Cheese-like 

 odor produced. 



Coagulated serum not liquefied. 



Hydrogen sulfide is produced. 



Acid from glucose, fructose, maltose and 

 glycerol. 



Ammonia, volatile amines, alcohol and 

 formic, butyric and lactic acids are pro- 

 duced. 



Nitrites not produced from nitrates. 



Neutral red and safranin are reduced. 



Anaerobic. 



Optimum temperature, 37° C. Killed at 

 70° C. 



Pathogenic. Guinea pigs killed in 24 hours 

 by intraperitoneal inoculation. 



Source: Isolated from feces in cases of 

 infantile diarrhea; also from soil from 

 French West Africa. 



Habitat: Found in intestines of children. 

 Rather uncommon. 



6. Eubacterium pseiitlotortuosuiii Pr6- 

 vot, 1947. (Ann. Inst. Past., 73, 1947, 409.) 



pseu.do.tor.tu.o'sum. Gr. adj. pseudes 

 false; L. adj. tortuosus full of windings, a 

 specific epithet; M.L. adj. pseudotortuosum 

 not the true {Eubacterium) tortuosum. 



Straight or curved rods, 0.4 to 0.5 by 3.0 

 to 4.0 microns, occurring in twisted chains 

 or wavy filaments. Non-motile. Gram-posi- 

 tive (decoloring easily). 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: Liquefaction in 3 days. 



Deep agar colonies: Lenticular. Gas is 

 produced. 



Glucose broth: Abundant turbidity and 

 gas. 



Peptone broth: Turbid. 



Milk: No coagulation. 



Coagulated proteins not attacked. 



Hydrogen sulfide is produced. 



Acid from glucose, fructose, maltose, 

 sucrose, lactose, galactose, sorbitol and 

 starch. 



Ammonia, formic, butyric and lactic 

 acids, volatile amines, alcohol, aldehyde, 

 ketone and acetylmethylcarbinol are pro- 

 duced. 



Nitrites produced from nitrates. 



Neutral red and safranin are reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Optimum pH, 7.4. 



Not pathogenic for guinea pigs or mice. 



Source: Isolated from a case of purulent, 

 acute appendicitis. 



Habitat: Found in human intestines. Un- 

 common. 



7. Eubacterium tortuosum (Debono, 

 1912) Pr^vot, 1938. (Bacillus tortuosus De- 

 bono, Cent. f. Bakt., I Abt., Orig., 6£, 1912, 

 233; Pr^vot, Ann. Inst. Past., 60, 1938, 295.) 



tor.tu.o'sum. L. adj. tortuosus full of 

 windings. 



Straight rods of medium size, with 

 rounded ends, occurring in twisted chains. 

 Non-motile. Gram-positive. 



Slight gas production in culture media. 



Gelatin: Granular growth. No liquefac- 

 tion. 



Deep agar colonies: Small, lenticular or 

 irregular, grayish. Little gas produced. 



Glucose broth: Uniform turbidity. Vis- 

 cous, coherent sediment. Gas produced. 



Milk: Acidified; no coagulation. 



Coagulated proteins not digested. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid and gas from glucose, lactose, su- 

 crose, galactose, fructose, maltose, manni- 

 tol and starch. 



Ammonia, acetylmethylcarbinol and for- 

 mic, propionic and lactic acids are produced. 



Nitrites not produced from nitrates. 



Neutral red reduced. 



Anaerobic. 



Not pathogenic. 



Source: Isolated from human feces. 



Habitat: Found in the human intestine. 

 Rather uncommon. 



