556 



ORDER IV. EUBACTERIALES 



8. Eubacteriuni quintum Prevot, 1940. 

 (Anaerob No. V, Rodella, Ztschr. f. Hyg., 

 U, 1902, 475; Prevot, Man. d. Classif. et d. 

 Determ. d. Bact. Anaerobies, 1940, 65.) 



quin'tum. L. adj. quintus fifth. 



More or less thick rods, of variable length, 

 with rounded ends. Non-motile. Gram-posi- 

 tive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: Liquefaction in 5 to 7 days. 



Deep agar colonies: Opaque centers. Gas 

 is produced. 



Glucose broth: Rapid turbidity. 



Milk: Digested in 2 to 3 days after coagu- 

 lation. 



Coagulated serum slowly digested. 



Anaerobic. 



Pathogenic. Guinea pigs killed in 48 hours 

 after subcutaneous injection. 



Source: Isolated from cases of infantile 

 diarrhea. 



Habitat: Found in the intestines of chil- 

 dren. Uncommon. 



9. Eubacteriuni liiiiosum (Eggerth, 

 1935) Prevot, 1938. (Bacteroides limosus 

 Eggerth, Jour. Bact., 30, 1935, 290; Prevot, 

 Ann. Inst. Past., 60, 1938, 295.) 



li.mo'sum. L. adj. limosus full of slime, 

 slimy. 



Rods, 0.5 to 1.5 by 1.0 to 5.0 microns; the 

 average length is 3.0 to 4.0 microns. Pleo- 

 morphic. Occur as short ovoids and as 

 wedge-shaped bacilli; curved, hooked and 

 bifid forms are numerous. Metachromatic 

 granules are absent. Non-motile. Gram- 

 positive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: Slow liquefaction. 



Glucose agar and blood agar colonies: 2 

 to 4 mm in diameter, raised, cream-colored, 

 mucoid, adherent. No hemolysis on blood 

 agar. 



Glucose broth: Cloudy with a heavy, 

 mucoid sediment; the pH reaches 4.8. 



Milk: Unchanged. 



Coagulated proteins not attacked. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid and gas from glucose, fructose, man- 

 nitol, adonitol, erythritol, de.xtrin and tre- 



halose. A slight acidity (pH 6.0 to 6.5) de- 

 velops without detectable gas in aesculin, 

 cellobiose, glucosamine, inulin, glycogen, 

 maltose, mannose, methylmannoside, raffi- 

 nose, salicin, starch and sucrose. No acid 

 from amygdalin, arabinose, dulcitol, galac- 

 tose, glycerol, inositol, lactose, melezitose, 

 methylglucoside, rhamnose, sorbitol or 

 .xjdose. 



Gas and volatile acid, of which butyric 

 acid is a main component, are produced 

 from glucose; traces of lactic acid are also 

 produced (Pederson, Jour. Bact., 50, 1945, 

 478). 



Nitrites not produced from nitrates. 



Anaerobic. 



Optimum temperature, 37° C. 



Non-pathogenic for white mice and rab- 

 bits. 



Relationship to other species: A culture 

 of this organism supplied to Dr. H. A. Bar- 

 ker has been carefully studied. Barker (per- 

 sonal communication, November, 1955) 

 states that acetic and n -butyric acids are 

 produced from glucose and lactate and 

 that this organism should be transferred to 

 the genus Butyribacterium. 



Source: One strain, obtained as a single- 

 cell culture, was isolated from human feces. 



Habitat: Found in human feces and pre- 

 sumabl}^ in the feces of other warm-blooded 

 animals. 



10. Eubacteriuni ethylicum Prevot, 

 1938. {Bacillus gracilis ethylicus Achalme 

 and Rosenthal, Compt. rend. Soc. Biol., 

 Paris, 58, 1906, 1025; Prevot, Ann. Inst. 

 Past., 60, 1938, 295.) 



e.thy'li.cum. Gr. noun ether ether; M.L. 

 neut.n. ethyl the ethyl radical; M.L. adj. 

 ethylicus pertaining to ethyl. 



Slender, straight or curved rods occurring 

 singly, in pairs or in short chains. Granular. 

 Non-motile. Gram-positive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: No liquefaction. 



Deep agar colonies: At first punctiform, 

 spreading to 2 mm in diameter; irregular. 



Glucose broth: Flocculent growth which 

 precipitates, leaving the medium clear. 

 Ammonia is produced. 



Peptone broth: Same as for glucose broth. 



