FAMILY X. LACTOBACILLACEAE 



557 



Milk: Coagulated in 5 days, followed by 

 digestion. Gas produced. 



Indole not produced. 



Glucose, fructose, sucrose, lactose, man- 

 nitol, starch and glycerol are fermented 

 with the production of gas, acid (acetic and 

 butyric) and ethanol. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for rabbits and guinea pigs, 

 which are killed after subcutaneous inocu- 

 lation. 



Source: Isolated from a case of gastritis. 



Habitat: Found in the human stomach. 

 Uncommon. 



11. Eubacterium ureolyticuni Huet 



and de Cadore, 1954. (Ann. Inst. Past., 86, 

 1954, 242.) 



u.re.o.ly'ti.cum. M.L. fem.n. urea urea; 

 Gr. adj. lyticus dissolving; M.L. adj. ureo- 

 lijticus urea-digesting. 



Large rods, 0.8 b}^ 3.0 microns. Non-mo- 

 tile. Gram-positive. 



Gas produced in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Irregular, snowflake- 

 like. 



Glucose broth: Abundant growth. Gas is 

 produced. 



Peptone broth: Poor growth. 



Milk: Coagulated. 



Proteins not attacked. 



Hydrogen sulfide produced abundantly. 



Acid from lactose, maltose, fructose, glu- 

 cose and galactose. 



Volatile amines, aldehydes and butyric, 

 acetic and lactic acids, but not alcohol or 

 ketones, are produced. 



Nitrites produced from nitrates. 



Produces an extremely active urease. 



Neutral red, safranin and phenosafranin 

 are reduced. 



Anaerobic. 



Source: Isolated from feces from a sheep. 



Habitat: Unknown. 



12. Eubacterium biforme (Eggerth, 

 1935) Pr^vot, 1938. (Bacteroides biformis 

 Eggerth, Jour. Bact., 30, 1935, 283; Pr^vot, 

 Ann. Inst. Past., 60, 1938, 295.) 



bi.for'me. L. adj. biformis two-formed. 

 Short, ovoid rods, 0.7 by 1.5 microns, oc- 



curring singly, in pairs or in short chains. 

 Non-motile. Gram-positive. 



Gas produced in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular, 2 to 3 mm 

 in diameter. 



Glucose broth: Turbid. Acidified. 



Milk: Acidified and coagulated. 



Coagulated proteins not attacked. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid and abundant gas from glucose, 

 fructose, maltose, galactose, mannose, lac- 

 tose and dextrin. 



Nitrites not produced from nitrates. 



Anaerobic. 



Pathogenic for rabbits but not for mice. 



Relationship to other species: Pederson 

 (personal communication, March, 1945) 

 points out that this organism may have 

 been a Lactobacillus , but that its relation- 

 ships are not definitely known. 



Source: Isolated from human feces. 



Habitat: Found in the human intestine. 

 Uncommon. 



13. Eubacterium nitritogenes Pr6vot, 

 1940. (Compt. rend. Soc. Biol., Paris, ISJt, 

 1940, 353.) 



ni.tri.to'ge.nes. M.L. neut.n. nitritum 

 nitrite; Gr. v. gennaio to produce; M.L. 

 adj. nitritogenes nitrite-producing. 



Straight, thick rods, 1.0 by 2.5 to 4.0 mi- 

 crons, with rounded ends. Non-motile. 

 Gram-positive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. Gas pro- 

 duced. 



Gluco.se broth: Abundant turbidity and 

 gas. 



Peptone broth: Turbid; gas is produced. 



Milk: No coagulation. 



Coagulated proteins not attacked. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose and fructose; sucrose 

 and lactose are very weakly fermented. 



Ammonia, volatile amines and propionic, 

 butyric and lactic acids are produced. Ace- 

 tylmethylcarbinol not produced. 



Nitrites produced from nitrates. 



