558 



ORDER IV. EUBACTERIALES 



Neutral red reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Optimum pH, between 6.5 and 7.8. Acid- 

 tolerant but not acidophilic. 



Not pathogenic for guinea pigs or mice. 



Source: Isolated from a peptic digest of 

 meat in an acid medium. 



Habitat: Unknown. 



14. Eiibacterium aerofaciens (Eggerth, 

 1935) Prevot, 1938. (Bacteroides aerofaciens 

 Eggerth, Jour. Bact., 30, 1935, 282; Prevot, 

 Ann. Inst. Past., 60, 1938, 295.) 



a.e.ro.fa'ci.ens. Gr. mas.n. aer air, gas; 

 L. V. facio to make, produce; M.L. part. adj. 

 aerofaciens gas-producing. 



Ovoid rods, 0.4 to 2.0 by 2.0 to 3.0 microns, 

 with rounded or pointed ends, occurring in 

 chains of two to ten elements. Non-motile. 

 Gram-positive. 



Gas produced in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies : 1 to 2 mm in diameter, 

 coherent. 



Glucose broth: Slightly turbid. 



Milk: Acidified; not coagulated. 



Coagulated proteins not attacked. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid and gas from glucose, maltose, fruc- 

 tose, galactose, sucrose and lactose. 



Nitrites not produced from nitrates. 



Anaerobic. 



Pathogenic for mice. 



Source: Isolated from human feces. 



Habitat: Found in the human intestine. 

 Rather uncommon. 



15. Eubacteriiim parvum Prevot, 1938. 

 (Coccobacillus anaerobicus parvus Chouke- 

 vitch, Ann. Inst. Past., 25, 1911, 256; Pre- 

 vot, Ann. Inst. Past., 60, 1938, 295.) 



par'vum. L. adj. parvus small. 



Small, ovoid rods, 0.5 by 1.0 to 1.5 mi- 

 crons, occurring singly or in pairs; filamen- 

 tous forms may occur. Non-motile. Gram- 

 positive. 



Gas not produced in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Small, lenticular. 



Glucose broth: Abundant turbidity and 

 sediment. 



Milk: Coagulated in 20 to 25 days; some 

 strains coagulate more rapidly ; no digestion. 



Coagulated proteins not attacked. 



Acid from glucose, fructose, maltose, 

 galactose and lactose. 



Ammonia, aldehydes, alcohols, ketones, 

 acetylmethylcarbinol and formic, butyric 

 and lactic acids are produced. 



Nitrites not produced from nitrates. 



Neutral red and phenosafranin reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Not pathogenic for laboratory animals. 



Source: Isolated from the large intestine 

 of a horse; also from a case of acute appendi- 

 citis. 



Habitat: Found in the intestines of foals 

 and of man. Uncommon. 



16. Eubacterium crispatum Brygoo 



and Aladame, 1953. (Ann. Inst. Past., 8^, 

 1953, 640.) 



cris.pa'tum. L. part. adj. crispatus curled, 

 crisped. 



Straight or slightly curved rods, 1.0 by 

 3.0 microns, occurring sometimes singly 

 but usually in rather long chains having a 

 twisted appearance and numerous loops; 

 the length of each chain varies from 20 to 

 40 microns. Non-motile. Gram-positive. 



Gas not produced in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Punctiform. 



Glucose broth: Abundant, uniform tur- 

 bidity. 



Peptone broth: Slightly turbid. 



Milk: Slowly coagulated; partially di- 

 gested. 



Coagulated proteins not attacked. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose, fructose, maltose, 

 sucrose, lactose, galactose and starch. No 

 acid from glycerol. 



Acetic acid and traces of lactic acid, but 

 not amines, aldehydes, ketones or acetone, 

 are produced. 



Nitrites not produced from nitrates. 



Sulfites not produced from sulfates. 



Neutral red reduced. Safranin and pheno- 

 safranin not reduced. 



Anaerobic. 



Not pathogenic for guinea pigs or mice. 



