FAMILY X. LACTOBACILLACEAE 



561 



crons; possess fusiform or spherical swell- 

 ings. Non-motile. Gram-positive. 



Gas produced in culture media. 



Gelatin: Liquefaction. 



Deep agar colonies : Large (3 to 5 mm in 

 diameter), flocculent, arborescent. Medium 

 broken bj' gas. Rancid odor. Acidified. 



Glucose broth: Turbid. Flaky sediment. 

 Abundant gas. Rancid odor. Acidified. 



iNIilk: Acidified; not coagulated. Gas is 

 produced. Rancid odor. 



Coagulated proteins not attacked. 



Hj'drogen sulfide is produced. 



Acid from glucose, maltose, sucrose, sorbi- 

 tol, mannitol and glycerol. 



Ammonia, alcohols, acetylmethylcarbinol 

 and formic, butj^ric and lactic acids are 

 produced. 



Nitrites not produced from nitrates. 



Neutral red and phenosafranin not re- 

 duced. 



Anaerobic. 



Optimum temperature, 37° C. Killed at 

 60° C. 



Not pathogenic or only slightlj- so; causes 

 minor abscesses in rabbits. 



Source: Isolated from the mouth of a 

 breast-fed infant; also from pond mud. 



Habitat: Unknown. 



2. Catenabacteriuni filaineiitosuin 



Prevot, 1938. (Jungano, Compt. rend. Soc. 

 Biol., Paris, 66, 1909, 112 and 122; Prevot, 

 Ann. Inst. Past., 60, 1938, 295.) 



fi.la.men.to'sum. L. nounfilum a thread; 

 ALL. noun filamentum a filament; M.L. adj. 

 fikimentosus full of filaments. 



Large rods with rounded ends. Pleo- 

 morphic, occurring either as short and swol- 

 len or long and curved forms. Bifurcation. 

 Non-motile. Gram-positive. 



Slight amount of gas produced (on agita- 

 tion) in culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Rather large, white, 

 lenticular. Slight gas. 



Glucose broth: Turbid. Slight gas. 



Peptone broth: Slight turbidity. 



Milk: Coagulated. 



Coagulated proteins not attacked. 



Indole not produced. 



Acid from glucose, fructose, maltose, ga- 

 lactose, lactose and sucrose. 



Ammonia, volatile amines, alcohol, ace- 

 tylmethylcarbinol, acetic and lactic acids 

 and traces of formic and propionic acids are 

 produced. 



Nitrites not produced from nitrates. 



Neutral red reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Not pathogenic for guinea pigs or mice. 



Source: Isolated from the intestine of a 

 rat; also from cases of acute appendicitis, 

 lung abscess, putrid pleurisy and uterine 

 suppuration. 



Habitat: Found in the intestine of the 

 rat and in the natural cavities of man. Com- 



3. Catenabacteriuni contortuin Pre- 

 vot, 1947. (Ann. Inst. Past., 73, 1947, 414.) 



con.tor'tum. L. adj. confortus twisted. 



Rods, 0.5 to 0.7 by 3.0 to 4.0 microns, oc- 

 curring in long, twisted chains of 30 to 50 

 or more elements. Non-motile. Gram-posi- 

 tive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. Gas is 

 produced. 



Peptone broth: Little or no growth. 



Glucose broth: Slowly turbid; growth 

 occurs as a viscid, semifluid mass. Gas is 

 produced. 



Milk: No coagulation. 



Coagulated proteins not attacked. 



Indole is produced. 



Acid and gas from glucose, fructose, malt- 

 ose, galactose, sucrose, xylose and arabi- 

 nose. 



Ammonia, formic, propionic and lactic 

 acids, volatile amines, aldehydes, alcohols 

 and acetylmethjdcarbinol are produced. 



Nitrites not produced from nitrates. 



Neutral red reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Neither toxin nor hemolysin is produced. 



One strain pathogenic for guinea pigs, 

 causing death in 48 hours. 



Source: Isolated from two cases of putrid, 

 gangrenous appendicitis. 



Habitat: Found in human intestines. Un- 



