564 



ORDER IV. EUBACTERIALES 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. Some gas 

 bubbles are produced. 



Blood media: No hemolysis. 



Glucose broth: Turbid. Acidified. Gas. 

 Rancid odor. 



Peptone broth: Very poor growth. 



Milk: Coagulated. No digestion. 



Coagulated proteins not attacked. 



Indole not produced. 



Acid and gas from glucose, maltose, ga- 

 lactose, sucrose, lactose and mannitol. 



Ammonia, acetylmethylcarbinol and for- 

 mic and acetic acids are produced. 



Nitrites not produced from nitrates. 



Neutral red reduced temporarily. Safranin 

 not reduced. 



Anaerobic. 



Grows at temperatures from 22° to 37° C. 

 Killed at 56° C. 



Optimum pH, between 7.0 and 8.0. 



Toxin, but no hemolysin, is produced. 



Pathogenic. Fatal for guinea pigs and 

 rabbits. 



Comments: Prevot iloc. cit.) recognizes 

 two varieties of this species which differ 

 from the parent strain with respect to cer- 

 tain cultural and biochemical characters. 



Source: Isolated in association with mas- 

 toiditis, otitis, pulmonary gangrene, putrid 

 pleurisy, appendicitis, intestinal infections, 

 balanitis, liver abscess, osteomyelitis, ■sep- 

 ticemia, urinary infections, etc. 



Habitat: Found in the natural cavities 

 of man and animals; also found in sea water. 

 Very common. 



2. Raniibacterium pleuriticum Prevot 

 et al., 1947. (Prevot, Raynaud and Digeon, 

 Ann. Inst. Past., 73, 1947, 481.) 



pleu.ri'ti.cum. Gr. fem.n. pleura a rib; 

 M.L. adj. pleuriticus pertaining to pleurisy. 



Rods resemble those found in Raniibac- 

 terium ramosum; the Y-shaped forms pre- 

 dominate. Non-motile. Gram-positive. 



Gas but no odor produced in culture me- 

 dia. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. Gas is 

 produced. 



Glucose broth: Abundant turbidity. 

 Rather coherent, viscous sediment. Gas is 

 produced. 



Peptone broth: Slightly turbid. Gas is 

 produced. 



Milk: No coagulation. 



Coagulated proteins not attacked. 



Indole not produced. 



Acid from glucose and galactose. One 

 strain ferments sucrose and maltose. 



Ammonia, traces of hydrogen sulfide, 

 alcohol, ketones, volatile amines and acetic, 

 valerianic and lactic acids are produced. 



Acetjdmethylcarbinol not produced. 



Nitrites not produced from nitrates. 



Neutral red and safranin reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Optimum pH, 7.8. 



Not pathogenic for guinea pigs or mice. 



Source : Isolated from two cases of fetid, 

 purulent pleurisy. 



Habitat: Found in the natural cavities of 

 man, especially the respiratory tract. Not 

 common. 



3. Ramibacterium ramosoides (Rune- 

 berg, 1908) Prevot, 1938. (Bacilhis ramo- 

 soides Runeberg, Arb. a. d. path. Inst. d. 

 Univ. Helsingfors, 2, 1908, 271; see Cent. f. 

 Bakt., I Abt., Ref., 43, 1909, 665; Prevot, 

 Ann. Inst. Past., 60, 1938, 296.) 



ra.mo.so.i'des. L. mas.n. ramus a branch; 

 L. adj. ramosus full of branches; Gr. noun 

 eidus form, shape; M.L. adj. ramosoides 

 branch-like. 



Cells occur either as ovoids in small chains 

 or as long elements showing what appears 

 to be false branching; sometimes spherical. 

 Non-motile. Gram-positive. 



Gas produced in moderate amounts in 

 culture media. 



Gelatin: No liquefaction. 



Deep agar colonies: Lenticular. Some 

 gas is produced. 



Brain media: Fetid odor produced. 



Blood media: Hemolysis. Fetid odor pro- 

 duced. 



Glucose broth: Turbid. Viscous, glairy 

 sediment. Acidified. Gas is produced. 



Peptone broth: Moderate growth. 



Milk: Coagulation in 8 days. 



Coagulated proteins not attacked. 



Indole is produced. 



Acid from glucose, maltose and lactose. 



