568 



ORDER IV. EUBACTERIALES 



Orig., 59, 1911, 101 ; Bacieroides tenuis Bergey 

 et al., Manual, 1st ed., 1923, 263; Cillobac- 

 terium spatulif or me Frevot, Ann. Inst. Past., 

 60, 1938, 297.) 



te'nu.e. L. adj. tenuis slender. 



Straight or curved rods with square ends; 

 one of the ends is usually enlarged, thus 

 giving the cells the appearance of spatulas. 

 Motile. Gram-positive. 



Gas produced in culture media. 



Gelatin: Liquefaction. 



Deep agar colonies: Circular. Small 

 amount of gas is produced. 



Milk: Coagulated then digested. 



Coagulated protein is attacked. 



Indole is produced. 



Acid and gas from glucose. 



Anaerobic. 



Optimum temperature, 37° C. 



Pathogenicity unknown. 



Source: Isolated from feces from a dog. 



Habitat: Unknown. 



5. Cillobacterium multiforme (Dis- 

 taso, 1911) Prevot, 1938. (Bacillus multi- 

 formis Distaso, Cent. f. Bakt., I Abt., Orig., 

 59, 1911, 101; not Bacillus jtiultiformis van 

 Senus, Dissert., Leiden, 1890; Prevot, Ann. 

 Inst. Past., 60, 1938, 297.) 



mul.ti.for'me. L. adj. multus much, 

 many; L. noun /orma shape; M.L. adj. 7nul- 

 tiformis many-shaped. 



Straight or curved, thick, pleomorphic 

 rods, 3 microns long. Motile. Gram-positive. 



Gas and odor produced in culture media. 



Gelatin: Liquefaction. 



Deep agar colonies: Lenticular. Abundant 

 gas is produced. 



Glucose broth: Turbid. Gas is produced. 

 Putrid odor. 



Milk: Coagulated then digested. 



Coagulated protein is attacked. Fibrin 

 and coagulated serum are digested. 



Indole is produced in trace amounts. 



Acid and gas from glucose, lactose, fruc- 

 tose, maltose, galactose, sorbitol, mannitol 

 and glycerol. 



Ammonia, hydrogen sulfide and formic, 

 butyric and lactic acids are produced. 



Nitrites not produced from nitrates. 



Neutral red reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Neither toxin nor hemolysin is pro- 

 duced. 



Not pathogenic for guinea pigs. 



Source: Isolated from the feces of a dog; 

 also from soil from Equatorial Africa. 



Habitat: Unknown. 



6. Cillobacterium combesii Prevot and 



Laplanche, 1947. (Ann. Inst. Past., 73, 1947, 

 687.) 



com.be'si.i. M.L. gen. noun combesii of 

 Combes; named after Combes. 



Straight rods, 0.7 by 3.0 to 4.2 microns, 

 with square ends, occurring singly or in 

 pairs, more often in chains of 3 to 10 ele- 

 ments. Motile by means of slow undula- 

 tions. Gram-positive. 



Gas and odor produced in culture media. 



Gelatin: Liquefaction in 5 days. 



Deep agar colonies: Irregular; arbores- 

 cent or like tufts of cotton. Gas is produced. 



Glucose broth : Abundant turbidity which 

 forms a viscous, zoogloea-like mass. Fetid 

 gas is produced. 



Peptone broth: Turbid. 



Milk: Coagulated in 8 days, then digested. 



Coagulated proteins not attacked. 



Carbohydrates not attacked. 



Ammonia, hydrogen sulfide, alcohol, 

 traces of acetylmethylcarbinol and formic, 

 butyric and valerianic acids are produced. 



Nitrites not produced from nitrates. 



Neutral red and safranin are reduced. 



Anaerobic. 



Optimum temperature, 37° C. 



Toxin not produced. 



Not pathogenic for guinea pigs or mice. 



Source: Isolated from forest soil from 

 French West Africa. 



Habitat: Unknown. 



