58G 



ORDER IV. EUBACTERIALES 



transmitted light and bluish white by re- 

 flected light. 



Bovine blood serum slants: Pit-like or 

 more general areas of liquefaction. 



Serum bouillon: Cloudy with fine floccu- 

 lent grayish flakes that form a sediment 

 like a streptococcus culture. 



Milk: Coagulation after 48 hours at 37° C, 

 with acid at bottom of tube. Separation of 

 whey and peptonization. 



Indole not produced. 



Acid produced in serum bouillon from 

 glucose, sucrose, lactose and xylose but 

 not from raffinose, inulin, mannitol or sali- 

 cin. 



Nitrites not produced from nitrates (Mer- 

 chant, Jour. Bact., 30, 1935, 108). 



Beta hemolytic; not hemoglobinophilic, 

 though growth is favored by proteins as 

 egg albumen, serum or blood (Brown and 

 Orcutt, op. cit., 1920, 244). 



Aerobic as well as anaerobic growth. 



Optimum temperature, 37° C. Growth 

 range, 20° to 40° C. 



Intravenous injection of rabbits and mice 

 usually fatal. 



Toxin and a heat-labile hemolysin are 

 produced (Lovell, Jour. Path, and Bact., 

 45, 1937, 339). 



Source: Isolated from bovine pus (Lucet, 

 op. cit., 1893, 327); also isolated from path- 

 ological processes in man (Forgeot, Halbron 

 and Levy-Bruhl, Ann. Inst. Past., 65, 1940, 

 326; also see Ballard, Upsher and Seeley, 

 Amer. Jour. Clin. Path., 27, 1947, 209). 



Habitat: Found in abscesses in cattle, 

 swine and other warm-blooded animals, 

 including man. 



8. Corynebacterium murisepticuni 



von Holzhausen, 1927. (Cent. f. Bakt., I 

 Abt., Orig., 105, 1927, 94.) 



mu.ri.sep'ti.cum. L. noun »ius, muris a 

 mouse; Or. adj. septicus septic; L. adj. tnuri- 

 septicvs mouse-poisoning (-infecting). 



Slender rods, 1.2 to 1.5 microns in length, 

 with polar granules. Grow out into long 

 filaments. Non-motile. Gram-positive. 



Gelatin stab: Feeble growth, with fimbri- 

 ate outgrowth along line of puncture. 



Egg glycerol broth: Good growth. 



Loeflfler's blood serum: Good growth. 



Broth: Turbid. 



Litmus milk: Acid. No coagulation. 



Potato: Good growth. 



Indole not produced. 



Hydrogen sulfide produced. 



Acid from glucose, fructose, galactose, 

 maltose, lactose, sucrose, inulin and man- 

 nitol. Arabinose and isodulcitol are not 

 attacked. 



Action on nitrates not reported. 



Aerobic, facultative. 



Optimum temperature, 37° C. 



Pathogenic for mice. 



Habitat: Septicemia in mice. 



9. Corynebacterium renale (Migula, 

 1900) Ernst, 1905. (Bacillus renalis bovis 

 Bollinger, in Enderlen, Zeit. f. Tiermed., 

 17, 1890, 346; Bacterium renale Migula, Syst. 

 d. Bakt., 2, 1900, 504; Ernst, Cent. f. Bakt., 

 I Abt., Orig., 40, 1905, 80.) 



re.na'le. L. adj. rewaZz's pertaining to the 

 kidne^^s. 



Description taken mainlj^ from Jones and 

 Little (Jour. Exp. Med., U, 1926, 11). 



Rods, 0.7 b}' 2.0 to 3.0 microns, occurring 

 usually in masses, rarely singly. Non-motile. 

 Bacteria from tissues not so pleomorphic 

 as those from the earlier transfer cultures, 

 although many show polar granules or swol- 

 len ends. Cultures grown in broth show coc- 

 coid forms and beaded rods with swollen 

 ends. Gram-positive. 



Gelatin: Grows poorly if at all. No lique- 

 faction. 



Agar: Small, punctiform colonies. 



Agar slants: Raised, grayish white and 

 dry growth (Jones and Little). Others say 

 cream-colored and moist. 



Blood serum slants: Fine, gray, puncti- 

 form colonies in 24 hours at 37° C. which 

 are a little larger than those on agar. Streak 

 scarcely 1 mm in width. Glistening and slimy 

 in fresh cultures. No liquefaction. 



Litmus milk: Reduction and coagulation 

 from the bottom. Slow digestion, becoming 

 alkaline. 



Broth: Sediment at end of 2 days with 

 clear bouillon above. 



Potato: Growth grayish white, later be- 

 coming a dingy yellow, turning the potato 

 brown. 



Acid from glucose. No acid from lactose, 

 sucrose, maltose or mannitol. Some strains 



