FAMILY XII. CORYXEBACTERIACEAE 



587 



ferment fructose and mannose (Merchant, 

 Jour. Bact., 30, 1935, 109). 



Aerobic, facultatively anaerobic. 



Optimum temperature, 37° C. 



Pathogenic for mice (Lovell and Cotchin, 

 Jour. Comp. Path, and Therap., 56, 1946, 

 205) and for rabbits (Feenstra, Thorp and 

 Gray, Amer. Jour. Vet. Res., 10, 1949, 12). 



No to.xin produced. 



Shows a close serological relationship with 

 Corynebacteriurn psuedotuberculosis (Mer- 

 chant). 



Source: Isolated from pyelonephritis in 

 cattle. 



Habitat: Occurs in purulent infections 

 of the urinary tract in cattle, sheep, horses 

 and dogs. 



10. Corynebacteriurn kutscheri (Mi- 



gula, 1900) Bergey et al., 1925. {Bacillus 

 pseudotuberculosis murium Kutscher, 

 Ztschr. f. Hyg., 18, 1894, 338; Bacterium 

 kutscheri Migula, Syst. d. Bakt., 2, 1900, 

 372; Bergey et al.. Manual, 2nd ed., 1925, 

 395.) 



kut'scher.i. M.L. gen. noun kutscheri of 

 Kutscher; named for Kutscher, the bac- 

 teriologist who first isolated this species. 



Rods, with pointed ends, staining irregu- 

 larly. Non-motile. Gram-positive. 



Gelatin colonies: Small, white, translu- 

 cent. 



Gelatin stab: No growth on surface. 

 White, filiform growth in stab. No lique- 

 faction. 



Agar colonies: Small, thin, yellowish 

 white, translucent, serrate. 



Agar slant: Thin, white, translucent. 



Loeffler's blood serum: Abundant growth. 

 Not peptonized. 



Broth: Slight turbidity. Crystals of am- 

 monium magnesium phosphate are pro- 

 duced. 



Litmus milk: Unchanged. 



Potato: No growth. 



Indole not produced. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature, 37° C. 



Source: Isolated from a cheesy mass in 

 the lung of a mouse. 



11. Corynebacteriurn phocac Sven- 



kerud et al., 1951. (Svenkerud, Rosted and 

 Thorshang, Nord. Vet. Med., S, 1951, 168.) 



pho'cae. M.L. noun Phoca a generic name 

 of seals; M.L. gen. noun phocae of Phoca. 



Description prepared by Prof. E. G. D. 

 Murray from the original publication by 

 Svenkerud et al. and from a study of cul- 

 tures supplied by these investigators. 



Rods, 0.4 to 0.6 by 0.7 to 2.0 microns, oc- 

 curring in frequently flexed chains of 3 to 7 

 or more cells, in linear, end to end pairs or 

 in pairs lying at an obtuse angle to each 

 other; occasionally single cells and very 

 long rod-shaped forms, 10 to 15 microns in 

 length, may occur, the latter sometimes 

 being curved. Non-motile. Gram-positive. 



Gelatin: No liquefaction. 



Agar colonies: Small (0.1 mm), circular, 

 smooth, moderately elevated, entire; at 

 first (24 hours) transparent and colorless, 

 later (48 hours) becoming enlarged (0.5 to 

 1.0 mm), opalescent and slightly white. 



Potassium tellurite medium: No growth. 



Peptone broth: Poor growth at 37° C. 

 and room temperature. 



Pneumo broth: Good growth at 37° C. 

 and room temperature; slight growth in 3 to 

 4 days at 4° C., becoming progressively 

 heavier and producing a turbidity in the 

 lower portion of the medium, thus leaving 

 a clear supernatant several mm in depth. 



Coagulated blood serum: No liquefaction. 



Litmus milk: No change. 



Indole not produced. 



Hydrogen sulfide not produced. 



Carbohydrate fermentation determina- 

 tions (except those of esculin, arbutin and 

 alpha-methyl-glucoside) performed in Hiss 

 serum sugar water. Acid from glucose, fruc- 

 tose, sucrose, maltose, trehalose and salicin. 

 Slight acid from xj-lose, galactose, lactose, 

 inulin, glycerol, inositol and mannitol. Acid 

 occasionally produced from rhamnose and 

 dextrin. No acid from arabinose, dulcitol, 

 sorbitol or arbutin. Glucose, fructose, su- 

 crose and salicin cause clotting of the me- 

 dium in 24 hours at 37° C. as does trehalose 

 in 48 hours; dextrin causes clotting l)y one 

 week. 



Starch, aesculin and alpha-met h3-l-glu- 

 coside are hydroljzed. 



Methyl red test positive. 



Acetylmethylcarbinol produced. 



