590 



ORDER IV. EUBACTERIALES 



little; Gr. adj. meiabolus changeable; M.L. 

 adj. pauro7netabolus little changeable, prob- 

 ably intended to mean producing little 

 change. 



Rods, 0.5 to 0.8 bj' 1.0 to 2.5 microns, oc- 

 curring singly, in pairs and in masses. Meta- 

 chromatic granules present. Non-motile. 

 Gram-positive. 



Gelatin stab: Slow liquefaction at surface. 



Agar colonies: White to gra}^, entire, cir- 

 cular, small, dry, somewhat granular. 



Agar slant: Filiform to arborescent, thick, 

 granular growth. 



Broth: Abundant, granular sediment but 

 no turbidity. Pellicle. 



Litmus milk: Alkaline. 



Potato: Thick, raised, dry, granular, 

 profuse, gray to light cream-colored growth. 



Indole not produced. 



Slight production of hydrogen sulfide. 



No action on the following carbohydrates : 

 glucose, lactose, sucrose, maltose, fructose, 

 mannitol, galactose, arabinose, xylose, 

 dextrin, salicin, rafiinose, trehalose, sorbi- 

 tol, inulin, dulcitol, glycerol, rhamnose, 

 adonitol, mannose, esculin and inositol. 



Nitrites not produced from nitrates. 



Aerobic. 



Slight alpha hemolysis. 



Non-pathogenic for guinea pigs. 



A special semi-solid medium, the main 

 nutritive constituents of which were pro- 

 teose peptone, rabbit serum, gelatin, minced 

 rabbit kidney and carbohydrates, was used 

 for the original isolation. An incubation 

 period of 4 to 7 days at 26° C. was necessary 

 for the initial isolation. Subsequent trans- 

 fers to ordinary beef -infusion agar grew out 

 in 24 to 48 hours. 



Source: Isolated from media inoculated 

 with the mycetome and ovaries of the bed- 

 bug, Cimex lectiilarius L. A very similar 

 diphtheroid strain was isolated from the 

 alimentary tract of the bagworm, Thyri- 

 dopteryx ephemerae/ ormis Haw. 



Habitat: Distribution in nature unknown. 



16. Corynebacterium nephridii Busing 

 et al., 1953. (Busing, Doll and Freytag, 

 Arch. f. Mikrobiol., 19, 1953, 77.) 



neph.ri'di.i. Gr. adj. nephridius of the 

 kidney; M.L. noun nephridium a nephri- 



dium, a little kidney; M.L. gen. noun neph- 

 ridii of a nephridium. 



Description taken from Busing and Frey- 

 tag (Zent. f. Bakt., I Abt., Orig., 160, 1954, 

 582). 



Slender rods, 0.4 to 0.5 by 1.0 to 2.0 mi- 

 crons, with no tendency toward filamentous 

 forms or chains; occasionally occur in a 

 palisade arrangement. Non-motile. Gram- 

 positive. 



Growth occurs on common culture media 

 on primary isolation. 



Gelatin: No liquefaction. 



Agar colonies: 1.5 mm in diameter, round, 

 smooth, moist, slimy, gray. 



Broth: Uniformly turbid; later becoming 

 slimy with a gray pellicle with stalactite- 

 like growths; sediment. 



Coagulated blood serum: No liquefaction. 



Litmus milk: Distinctly alkaline in 10 

 days. 



Indole not produced. 



Hydrogen sulfide not produced. 



No acid from carbohydrate media. 



Acetylmethylcarbinol not produced. 



Nitrites not produced from nitrates. 



Ammonia produced in peptone media. 



Urea not hydrolyzed. 



Blood agar: No hemolj^sis. 



Aerobic. 



Optimum temperature range, 15° to 37° C. 



Optimum pH range, 6.2 to 7.2. 



Source : Isolated from the medicinal leech 

 {Hirudo medicinalis) . 



17. Corynebacterium vesiculare Busing 

 et al., 1953. (Busing, Doll and Freytag, 

 Arch. f. Mikrobiol., 19, 1953, 76.) 



ve.si.cu.la're. M.L. adj. vesicularis per- 

 taining to a vesicle. 



Description taken from Busing and Frey- 

 tag (Zent. f. Bakt., I Abt., Orig., 160, 1954, 

 579). 



Slender, pleomorphic rods 0.4 by 3.0 to 

 8.0 microns; filamentous forms 30 microns 

 or more in length may also occur. Non- 

 motile. Gram-positive. 



Primary isolation accomplished on "fun- 

 gus" medium, but this species can be 

 adapted to grow on common culture media. 



Gelatin: No liquefaction. 



Agar colonies: After 2 days, 1 to 2 mm in 



