FAMILY XII. CORYXEBACTERIACEAE 



591 



diameter, round, smooth, moist, slimy, 

 orange to rust-red. 



Coagulated blood serum: No liquefaction. 



Litmus milk: Distinct alkalinity after 

 10 days. 



Indole not produced. 



Hydrogen sulfide not produced. 



No acid from carbohydrate media. 



Starch not hydrolyzed. 



Acetj'lmethylcarbinol not produced. 



Nitrites not produced from nitrates. 



Ammonia produced in peptone media. 



Urea not hj-drolyzed. 



Blood agar: No hemolysis. 



Aerobic. 



Optimum temperature range, 15° to 22° C. 



Optimum pH, 6.4. 



Source: Isolated from the medicinal leech 

 (Hinido niedicinalis). 



18. Corynebacteriuni insidiosum (Mc- 



Culloch, 1925) Jensen, 1934. (Aplanobacter 

 insidiosum 'SicCulloch, Phytopath., 15, 1925, 

 497; also see Jour. Agr. Res., 33, 1926, 502; 

 Jensen, Proc. Linnean Soc. New So. Wales, 

 59, 1934, 41.) 



in.si.di.o'sum. L. adj. insidiusus deceit- 

 ful, insidious. 



Rods, 0.4 to 0.5 by 0.7 to 1.0 micron. En- 

 capsulated. Non-motile. Gram-positive. 



Gelatin: Slow liquefaction. 



Beef agar colonies: Pale yellow, circular, 

 smooth, shining; edges entire; viscid. Blue 

 granules found on the medium. 



Milk: Coagulated after 16 to 20 days. No 

 digestion. An apricot-^yellow sediment is 

 deposited on the walls of the tube. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose, sucrose, lactose and 

 glycerol. 



Moderate diastatic action. 



Nitrites not produced from nitrates. 



Grows in 5 per cent salt. 



Aerobic. 



Optimum temperature, 23° C. Maximum, 

 31° C. 



Distinctive character: Bluish granules 

 produced in culture. 



Comments: Jensen (ibid., 42) reports that 

 he has found a non-infectious variety of 

 this species in grass land soil in Australia. 



Source: Isolated from diseased alfalfa 

 plants. 



Habitat: Vascular pathogen of alfalfa, 

 Medicago saliva . 



19. Corynebacteriuni sepedonicum 



(Spieckermann and Kotthoff, 1914) Skap- 

 tason and Burkholder, 1942. {Bacterium 

 sepedonicum Spieckermann {nomen nudum), 

 111. Landw. Zeitung, 33, 1913, 680; Bacterium 

 sepedonicum Spieckermann and Kotthoff, 

 Landw. Jahr., 46, 1914, 674; Skaptason and 

 Burkholder, Phytopath., 32, 1942, 439.) 



se.pe.do'ni.cum. Gr. adj. sepedonicus 

 leading to decaj'. 



Description taken from Stapp (Ztschr. f. 

 Par., 5, 1930, 756). 



Rods 0.3 to 0.4 by 0.8 to 1.0 micron. Pleo- 

 morphic. Non-motile. Gram-positive. 



Gelatin: Liquefaction slight. 



Agar colonies: Thin, smooth, translucent, 

 glistening, whitish, 2 to 3 mm in diameter. 



Broth: Weak growth. No pellicle. Light 

 sediment. 



Litmus milk: Little change in 6 weeks, 

 after which litmus is reduced. 



Indole not produced. 



Hydrogen sulfide production feeble, if at 

 all. 



Glucose, galactose, fructose, arabinose, 

 xylose, mannitol, glycerol and dulcitol are 

 utilized. 



Starch hydrolj'sis light. 



Grows in 4 per cent salt. 



Temperature relations: Optimum, be- 

 tween 20° and 23° C. Minimum, 4° C. Max- 

 imum, 31° C. 



Distinctive characters: Differs from Co- 

 njnebacterium michiganense in that it is 

 white to cream-colored on various media 

 and has a lower optimum temperature. 

 Corynebacterium michiganense does not in- 

 fect potatoes. 



Source: Stapp used 17 cultures isolated 

 from diseased potatoes. 



Habitat: Causes ring rot of ])otato tubers 

 in Germany. 



20. Corynebacteriuni huniiferuni Selis- 

 kar, 1952. (Colorado Farm and Home Re- 

 search, 2, 1952, 9.) 



hu.mi'fer.um. L. noun humus soil; L. v. 

 fero to bear; M.L. adj. humiferus soil-borne. 



