594 



ORDER IV. EUBACTERIALES 



and Pirone, 1942. (Phytopath., 32, 1942, 

 1080.) 



poin.set'ti.ae. M.L. fem.n. Poinsettia 

 name of a genus of flowering plants; M.L. 

 gen. noun poinsettiae of Poinsettia. 



Rods which average 0.3 to 0.8 by 1.0 to 

 3.0 microns. Pleomorphic, with some cells 

 8.5 microns in length. Encapsulated. Gran- 

 ules present. Motile by means of a single 

 (rarely 2) polar or lateral flagellum. Gram- 

 positive. 



Gelatin: Liquefaction. 



Loeffler's blood-serum: Liquefaction. 



Beef -extract agar colonies: Round, 

 slightly convex, 0.1 to 1.0 mm in diameter, 

 entire, smooth, non-viscid, colorless and 

 almost transparent. 



Potato glucose agar slants: Moderate 

 growth, filiform, glistening, non-viscid, 

 salmon- to flesh-colored. 



Beef -extract broth: Turbid in 24 hours; 

 abundant, pale salmon sediment. No pelli- 

 cle. 



Milk: Slight acidity but no other visible 

 change for 2 weeks, then a soft curd, reduc- 

 tion of litmus and complete peptonization. 



Indole not produced. 



Hydrogen sulfide not produced. 



Sodium hippurate not hydrolyzed. 



Moderate to abundant acid, but no gas, 

 from glucose, fructose, mannose, galactose, 

 sucrose, maltose, cellobiose, melibiose, 

 raffinose, glycerol, erythritol, salicin and 

 amygdalin; weak acid from arabinose, xy- 

 lose, lactose, trehalose, dextrin and 

 adonitol; no acid from rhamnose, fucose, 

 inulin, glycogen, mannitol, dulcitol, sorbitol 

 or inositol. 



Acetylmethylcarbinol not produced. 



Methyl red test negative. 



Starch hydrolyzed. 



No action on cellulose. 



Non-lipolytic. 



Tellurite reduced. 



Nitrites not produced from nitrates. 



Asparagine not utilized as carbon-nitro- 

 gen source. Uric acid not utilized; urea not 

 hydrolyzed. 



Aerobic. 



Growth occurs after 24 hours from 15° to 

 .36° C.; after 48 hours from 7° to 12° C. No 

 growth above 36° or below 7° C. at the end 

 of a week. 



Source: Fourteen cultures isolated from 

 diseased stems of poinsettia. Euphorbia 

 pulcherrima. 



Habitat: Causes a canker of stems and 

 spots on leaves of the poinsettia. 



27. Corynebacterium tritici (Hutchin- 

 son, 1917) Burkholder, 1948. (Pseudomonas 

 tritici Hutchinson, India Dept. of Agr., 

 Bact. Ser., 1, 1917, 174; Burkholder, in Man- 

 ual, 6th ed., 1948, 400.) 



tri'ti.ci. L. noun triticum wheat; M.L. 

 neut.n. Triticum generic name of wheat; 

 L. gen. noun tritici of wheat. 



Rods 0.8 by 2.4 to 3.2 microns. Motile by 

 means of a single polar flagellum. Gram- 

 positive. 



Gelatin: No liquefaction. 



Agar colonies: Bright yellow becoming 

 orange, glistening, moist, entire. Agar 

 brownish. 



Broth: Turbid. Thin pellicle. 



Milk: Yellow surface and yellow precipi- 

 tate. Little change. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose and lactose. 



Nitrites produced from nitrates. 



This species is very similar to and may 

 be identical with Corynebacterium rathayi 

 Dowson. 



Source: Isolated from slimy heads of 

 wheat in India. 



Habitat: Pathogenic on wheat, Triticum 

 aestivum. 



28. Corynebacterium flaccunifaciens 



(Hedges, 1922) Dowson, 1942. {Bacterium 

 flaccumfaciens Hedges, Science, 55, 1922, 

 433; also see Phytopath., 16, 1926, 20; Dow- 

 son, Trans. Brit. Myc. Soc, 25, 1942, 313.) 



flac.cum.fa'ci.ens. L. adj. flaccus flabby; 

 L. part. adj. faciens making; M.L. part. adj. 

 flaccumfaciens wilt-making. 



Rods 0.3 to 0.5 by 0.6 to 3.0 microns. Mo- 

 tile by means of a single polar flagellum; 

 also non-motile (Adams and Pugsley, Jour. 

 Dept. Agr. Victoria, 32, 1934, 306). Gram- 

 positive. 



Gelatin: Liquefaction feeble. 



Beef agar slants: Rather moderate 

 growth, glistening, flat, smooth, viscid and 

 yellow. 



