FAMILY XIII. BACILLACEAE 615 



bb. Glucose not utilized. Strong hydrolysis of gelatin. 



9. Bacillus badius. 



2. Growth on glucose agar definitely not so good as on agar. Scant, if any, growth 

 on soybean agar, 

 a. Casein hydrolyzed. Urease not produced. 



10. Bacillus firmus. 

 aa. Casein not hydrolyzed. Urease produced. 



11. Bacillus lentus. 



II. Sporangia definitely swollen. Spores ellipsoidal, rarely cylindrical, central to termi- 

 nal. Spore wall thick and easilj' stained. Remnants of sporangium sometimes adhering. 

 Gram- variable. 



A. Gas from carbohydrates. 



1. Acetylmethylcarbinol produced. Crystalline dextrins not produced from starch. 



12. Bacillus polymyxa. 



2. Acetylmethylcarbinol not produced. Crystalline de.xtrins produced from starch. 



13. Bacillus macerans. 



B. Gas not produced from carbohydrates. 



1. Saprophytic. Grow on ordinary media. 



a. Starch hydrolyzed. 



b. Indole and acetylmethylcarbinol produced. 



14. Bacillus alvei. 

 \bb. Indole and acetylmethylcarbinol not produced. 



c. Grows at 65° C. 



15. Bacillus stear other mophilus . 

 cc. Does not grow at 65° C. 



16. Bacillus circulans. 

 aa. Starch not hydrolyzed. 



b. pH of glucose broth cultures is less than 8.0. Grow in glucose broth 

 under anaerobic conditions. 

 c. Indole produced. Acid from glucose and mannitol with ammonium 

 salts as source of nitrogen. 



17. Bacillus later os-por us. 



cc. Indole not produced. Does not grow on carbohydrates with am- 

 monium salts as source of nitrogen. 



18. Bacillus pulvifaciens . 



bbV^H of glucose broth cultures is 8.0 or higher. Does not grow in glucose 

 /Broth under anaerobic conditions. 



19. Bacillus brevis. 



2. Parasitic. Do not grow on ordinary media. 



a. Cause of American foulbrood of honey bees. 



20. Bacillus larvae. 

 aa. Cause of the milky disease of Japanese beetles. 



Type A. 21. Bacillus popilliae. 

 Type B. 22. Bacillus lentimorbus . 

 III. Sporangia definitely swollen. Spores spherical or nearly so. Gram-variable. 



A. Grow on ordinary media at pH 6.0. Urea or alkaline conditions not necessary for 

 growth. 



1. Starch hydrolyzed. Grows in 10 per cent NaCl broth. 



2.3. Bacillus pantothenticus. 

 -2. Starch not hydrolyzed. Does not grow in 10 per cent NaCl broth. 



24. Bacillus sphaericus. 



B. Does not grow on ordinary media at pH 6.0. Urea or alkaline conditions necessary 

 for growth. 



25. Bacillus pasteurii. 



